Preparation method of multifunctional eccentric mesoporous silica core-shell nanoparticles
A technology of mesoporous silica and nanoparticles, which can be applied to preparations for in vivo tests, medical preparations with inactive ingredients, and pharmaceutical formulas, etc. The effect of stability, high drug loading and convenient operation
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Embodiment 1
[0035] Preparation of 80 nm fluorescence-labeled eccentric mesoporous silica-wrapped PAA and Fe3O4 core-shell nanoparticles:
[0036] (1) Synthesis of CTAB-protected Fe3O4 nanoparticles: take 500 μL, 10 mg mL -1 Oleic acid-protected 25 nm Fe3O4 nanoparticles dissolved in chloroform were mixed with 10 mL of 0.2 M CTAB and vigorously stirred at 32 °C for 30 min. Then the solution was warmed up to 60 oC to evaporate the organic solvent chloroform, kept stirring for 20 minutes and then cooled to room temperature. The excess CTAB molecules were washed twice with deionized water and centrifuged to collect CTAB-protected Fe3O4 nanoparticles.
[0037] (2) Preparation of eccentric PAA-coated Fe3O4 core-shell nanoparticles: Take the CTAB-protected Fe3O4 nanoparticles prepared in step (1) and add 5 mL of deionized water for ultrasonic dispersion. Add 40 μL PAA aqueous solution (0.2 g mL -1 ) and 60 μL NH 3 ·H 2 O solution (2 M), sonicate for half an hour each time. Then 120 mL of i...
Embodiment 2
[0040] Preparation of 100 nm fluorescence-labeled eccentric mesoporous silica-coated PAA and Fe3O4 core-shell nanoparticles:
[0041] (1) Synthesis of CTAB-protected ferric oxide nanoparticles: the specific operation is the same as step (1) in Example 1.
[0042] (2) Preparation of eccentric PAA-wrapped ferric oxide core-shell nanoparticles: the specific operation is the same as step (2) in Example 1.
[0043](3) Preparation of 100 nm fluorescence-labeled eccentric mesoporous silica-wrapped PAA and Fe3O4 core-shell nanoparticles: Take 15 mL of eccentric PAA-wrapped Fe3O4 core-shell nanoparticles prepared in step (2), Join 2 MNH 3 ·H 2 O solution to adjust the pH of the solution to 8, and then add 5 μL of FITC-silane precursor and 500 μL of 20% isopropanol solution of tetrahexyl orthosilicate (TEOS), wherein the isopropanol solution of TEOS was added every 30 min Add once and add in seven times. Then, the mixed solution was continuously stirred at room temperature for 24 h....
Embodiment 3
[0045] Preparation of 130 nm fluorescence-labeled eccentric mesoporous silica-wrapped PAA and Fe3O4 core-shell nanoparticles:
[0046] (1) Synthesis of CTAB-protected ferric oxide nanoparticles: the specific operation is the same as step (1) in Example 1.
[0047] (2) Preparation of eccentric PAA-wrapped ferric oxide core-shell nanoparticles: the specific operation is the same as step (2) in Example 1.
[0048] (3) Preparation of 130 nm fluorescence-labeled eccentric mesoporous silica-wrapped PAA and Fe3O4 core-shell nanoparticles: Take 15 mL of eccentric PAA-wrapped Fe3O4 core-shell nanoparticles prepared in step (2), Join 2 MNH 3 ·H 2 O solution to adjust the pH of the solution to 8, then add 5 μL of FITC-silane precursor and 750 μL of 20% isopropanol solution of tetrahexyl orthosilicate (TEOS), wherein the isopropanol solution of TEOS was added every 30 min Add once and add ten times. Then, the mixed solution was continuously stirred at room temperature for 24 h. After...
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