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In-vitro embryo cultivation method for measuring vitality of cynomorium seeds

A viability and embryo culture technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of not completely eliminating seed dormancy, declining seed viability, low seed germination rate, etc. , fast and safe rejection, good repeatability

Active Publication Date: 2013-07-24
甘肃凯源生物技术开发中心有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Cynomorium seeds are expensive, the grains are small, and have special dormancy characteristics, there are appendages on the surface of the seeds and the hard rate is high, and the seed embryo is embedded in the dense and hard endosperm. In the process of treating positive seeds, promoting their germination, and measuring their viability, the dormancy of the seeds cannot be completely broken, but problems such as low seed germination rate, decreased seed viability, and poor repeatability appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The first isolated embryo culture method for measuring the viability of Cynomorium cynomorium seeds comprises the following steps:

[0051] a) take wild fully mature Cynomorium cynomorium seeds 500g;

[0052] b) Cynomorium seeds are put into the cleaning machine, cleaned at a wind speed of 12.6m / s, then coarsely screened with a 1.62mm sieve, and then finely screened with a 0.60mm sieve to obtain clean seeds;

[0053] c) Removal of appendages: Soak in 1% NaOH solution for 3 minutes under stirring to obtain seeds without appendages, and the removal rate reaches 100%;

[0054] d) Hard fruit removal: Randomly select 400 seeds without appendages obtained in step c), conduct 4 repeated experiments of 100 seeds each time, and treat Cynomorium cynomorium seeds with 1mol / L NaOH solution for 5 hours;

[0055] e) Remove the seed coat and take out the seed kernel: squeeze the peel with tweezers, take out the seed kernel, and obtain the peeled seed kernel;

[0056] f) Culture of i...

Embodiment 2

[0060] The second method for culturing in vitro embryos used for the determination of Cynomorium seed viability comprises the following steps:

[0061] a) take wild fully mature Cynomorium cynomorium seeds 500g;

[0062] b) Clarity treatment: put Cynomorium cynomorium seeds into the cleaning machine, clean them with a wind speed of 10m / s, then use a 1.5 sieve to carry out coarse screening to the seeds, and then use a 0.40mm sieve to carry out fine screening to the seeds to obtain clean seed;

[0063] c) Removal of appendages: scald the seeds with hot water at 90°C, cool to room temperature and ensure that the seeds are soaked in water for 24 hours, then rub with nylon gauze, the removal rate is 96.7%;

[0064] d) Hard fruit removal: Randomly select 400 seeds without appendages obtained in step c), and conduct 4 repeated experiments of 100 seeds each time, and treat Cynomorium cynomorium seeds with 1.5mol / L NaOH solution for 4 hours;

[0065] e) Remove the seed coat and take ...

Embodiment 3

[0070] The third method for in vitro embryo culture for Cynomorium seed viability assay comprises the following steps:

[0071] a) take wild fully mature Cynomorium cynomorium seeds 500g;

[0072] b) Clarity treatment: Put Cynomorium cynomorium seeds into the cleaning machine, clean them with a wind speed of 15m / s, then use a 1.8 sieve to carry out coarse screening to the seeds, then use a 0.8mm sieve to carry out fine screening to the seeds to obtain clean seed;

[0073] c) Removal of appendages: scald the seeds with hot water at 100°C, cool to room temperature and ensure that the seeds are soaked in water for 24 hours, then rub with nylon gauze, the removal rate is 94%;

[0074] d) Hard fruit removal: Randomly select 400 seeds without appendages obtained in step c), conduct 4 repeated experiments of 100 seeds each time, and treat Cynomorium cynomorium seeds with 2.0mol / L NaOH solution for 3 hours;

[0075] e) Remove the seed coat and take out the seed kernel: squeeze the p...

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Abstract

The invention discloses an in-vitro embryo cultivation method for measuring the vitality of cynomorium seeds, and belongs to the field of quality identification and cultivation for noble traditional Chinese medicine seeds in desert regions. The method comprises the following steps of: moving seeds, removing appurtenants from the surfaces of the seeds, breaking hard solids, shelling the seeds, taking cores, cultivating in-vitro embryos and measuring the vitality. By the method, cleanness treatment, appurtenant removal, hard solid breaking and vitality measurement are quickly and effectively performed on the cynomorium seeds; heat injury and mechanical injury as well as death of the cynomorium seeds, which are caused by other treatment, are reduced; the working danger in an operation process is reduced; the working efficiency is high; the repetitiveness is high; and the method is suitable for measuring the vitality of the cynomorium seeds and pre-treating larger-size seeds before planting. The removal rate of the appurtenants on the surfaces of the cynomorium seeds is 94-100 percent; the vitality of the cynomorium seeds is 95-97 percent; and after the appurtenants are removed from the cynomorium seeds, the sprouting time is 10-day ahead.

Description

technical field [0001] The invention belongs to the field of quality identification and cultivation of precious Chinese medicinal material seeds in desert areas, and in particular relates to an in vitro embryo culture method for measuring the viability of Cynomorium cynomorium seeds. technical background [0002] Cynomorium songaricum is a perennial succulent parasitic herbaceous seed plant of Cynomorium (Cynomorium) in Cynomoriaceae (Cyno-moriaceae), and it is mostly parasitic on the roots of Nitraria (Zygophyllaceae) plants. Similar to the distribution system of Nitraria japonica, Cynomorium cynomorium mostly grows in the arid desert zone and the edge of the lake basin in the desert zone and the salinized sandy land in the alluvial and alluvial fan margin zone. It is listed as the top grade of precious Chinese medicinal materials, known as " "Desert ginseng". Modern research shows that Cynomorium also plays an important role in anti-tumor, regulating human immunity, delay...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王进罗光宏张勇祖廷勋颜霞
Owner 甘肃凯源生物技术开发中心有限责任公司
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