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Method for obtaining aseptic seedling by using pinus massoniana seed

A technology for aseptic seedlings and masson pine, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of insufficient root growth of seedlings, only 5-20%, recycling and waste, etc. Achieve the effect of saving seedling breeding costs, shortening emergence time, and simplifying experimental steps

Active Publication Date: 2013-07-24
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional aseptic seedling culture mostly uses agar powder (strip) medium, which needs to go through three steps of heating and dissolving agar powder (strip)—sterilization—cooling and coagulation, and the operation is relatively cumbersome; in addition, due to the existence of seed germination rate and pollution and other problems, the seedling rate of sterile seedlings can only reach 5-20%, making 80-95% of the culture medium become waste and difficult to recycle and reuse, resulting in waste; at the same time, the air permeability of agar is poor , seedling growth, especially root growth is not good enough

Method used

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  • Method for obtaining aseptic seedling by using pinus massoniana seed

Examples

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Effect test

Embodiment 1

[0027] Medium preparation

[0028] Cut a length╳width of about 3cm╳2cm sponge, put the sponge in tap water to fully absorb water, and use tweezers to put the sponge that has absorbed enough water into the tissue culture bottle, such as figure 1 As shown, the sponge is folded into a right angle and attached to the bottle wall (wall attachment height 1cm), placed vertically on the bottom of the tissue culture bottle, tightened the bottle cap, and sterilized at 121°C for 20 minutes;

[0029] Seed disinfection

[0030] Rinse with running water for 5 hours, soak in 0.5% potassium permanganate solution for 20 minutes, rinse with running water, sterilize the seeds with 0.1% mercury liter in an ultra-clean workbench for 8 minutes, and rinse with sterile water for 5 times;

[0031] Vaccination

[0032] Inoculate the sterilized seeds of step onto the sponge upper surface at the bottom of the parallel bottle in the sponge culture medium of step on the ultra-clean workbench;

[003...

Embodiment 2

[0038] Medium preparation

[0039] Cut the sponge with a length╳width of about 4.5cm╳2cm, put the sponge in tap water to fully absorb water, use tweezers to put the sponge that has absorbed enough water into the tissue culture bottle, and fold the sponge into a right angle to stick to the bottle wall (the height of the wall is 1.5cm ) placed vertically on the bottom of the tissue culture bottle, tightened the cap, and sterilized at 121°C for 20 minutes;

[0040] Seed disinfection

[0041] Rinse with running water for 6 hours, soak in 0.3% potassium permanganate solution for 30 minutes, rinse with running water, sterilize the seeds with 0.1% mercury liter in an ultra-clean workbench for 10 minutes, and rinse with sterile water 4 times;

[0042] Vaccination

[0043] Inoculate the sterilized seeds of step onto the sponge upper surface at the bottom of the parallel bottle in the sponge culture medium of step on the ultra-clean workbench;

[0044] Cultivation

[0045] Plac...

Embodiment 3

[0049] Medium preparation

[0050] Cut the sponge with a length╳width of 2.8cm╳1.5cm, put the sponge in tap water to fully absorb water, use tweezers to put the sponge that has absorbed enough water into the tissue culture bottle, and fold the sponge into a right angle to stick to the bottle wall (the wall height is 0.8 cm) placed vertically on the bottom of the tissue culture bottle, tightened the bottle cap, and sterilized at 121°C for 20 minutes;

[0051] Seed disinfection

[0052] Rinse with running water for 5 hours, soak in 0.5% potassium permanganate solution for 15 minutes, rinse with running water, sterilize the seeds with 0.1% mercury liter in an ultra-clean workbench for 9 minutes, and rinse with sterile water 5 times;

[0053] Vaccination

[0054] Inoculate the sterilized seeds of step onto the sponge upper surface at the bottom of the parallel bottle in the sponge culture medium of step on the ultra-clean workbench;

[0055] Cultivation

[0056] Place the...

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Abstract

The invention discloses a method for obtaining an aseptic seedling by using a pinus massoniana seed. By virtue of good water absorption and holding performance of sponge, the sterilized pinus massoniana seed is placed in the sponge to culture for 10-20 days to obtain the aseptic seedling which grows strongly. When the method disclosed by the invention is applied to culturing the pinus massoniana aseptic seedling, the time for the emerging of seedling is obviously reduced, and nursery stock growth, especially root growth is superior to that in agar culture; simultaneously, experimental procedure is simplified and culture efficiency is improved; in addition, as the sponge can be repeatedly used without generating wastes, seedling propagation cost is saved and environment is protected; and the aseptic seedling propagation method disclosed by the invention is economical, efficient, simple and convenient to operate, suitable for researche and production of biotechnologies such as the isolated culture of the pinus massoniana and transgenosis, and has important significance on the improved variety propagation of the pinus massoniana and biotechnology researches.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of pine pine, and in particular relates to a method for obtaining aseptic seedlings by using pine pine seeds. Background technique [0002] Pinus massoniana (Pinus massoniana) is an important dual-purpose tree species for resin and wood in southern my country. It has a wide range of uses and high economic value. In addition to the two major uses of wood and pine resin, the whole tree of Masson pine can be used, and the pollen, needles and bark are rich in antioxidant substances, which can be used as excellent anti-aging and anti-oxidation health products; pine nuts contain 30% oil, Paint, soap and lubricating oil can be made; crude oil can be extracted from cones; pine tar can be extracted from pine roots, and the precious Chinese medicinal material - Poria cocos can be cultivated. [0003] After more than 30 years of genetic improvement research, Guangxi has built a multi-level improved s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谭健晖陈虎杨章旗
Owner GUANGXI FORESTRY RES INST
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