Method for preparing bioactive filler through immobilization of anaerobic ammonia oxidizing bacterium
An anammox bacteria and anammox technology, applied in the field of water treatment, can solve the problems of long doubling time, low cell yield, long residence time, etc., and achieve short time consumption, good sedimentation performance, and recovery of biological activity. quick effect
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Embodiment 1
[0036] (1) Selection and treatment of activated carbon
[0037] Select granular activated carbon with a particle size greater than 1.0mm for mechanical crushing, and retain particles with a particle size of 0.3-0.5mm through sieving, soak in anammox bacteria liquid culture medium for 3 days, and put it in a constant temperature oven at 60°C for drying Dry, ensure that the surface of the granular activated carbon is not wet, take out 240ml of the dried activated carbon and cool it to normal temperature for later use.
[0038] (2) Treatment of anammox bacteria solution
[0039] Concentrate the existing anammox bacteria solution through a centrifuge (12000r / min, 4°C) and divide it into two 20ml bacterial suspensions;
[0040] (3) Acquisition of bacterial embedding solution
[0041] Weigh 2.7g of polyvinyl alcohol, add 10ml of ultrapure water, heat to dissolve for 15min, take it out and stir it evenly, then heat it for 5min, take it out, stir it evenly and cool to room temperatu...
Embodiment 2
[0050] (1) Selection and treatment of activated carbon
[0051] The selection and treatment of activated carbon are the same as in Example (1), and 1000 ml of the dried activated carbon is taken out and cooled to room temperature for later use.
[0052] (2) Treatment of anammox bacteria solution
[0053] The treatment of the anammox bacteria solution was the same as in Example (1), and two 100ml bacterial suspensions were prepared.
[0054] (3) Acquisition of bacterial embedding solution
[0055] Weigh 11.7g of polyvinyl alcohol, add 25ml of ultrapure water and heat to dissolve for 15min, take it out and stir evenly, then heat for 5min, take it out, stir evenly and cool to room temperature, mix 25ml of polyvinyl alcohol solution with 100ml of the prepared bacterial suspension After mixing, the mass concentration of anammox bacteria was 2%, and 5.2g of calcium carbonate was added, and an appropriate amount of ultrapure water was added, and 130ml of bacterial embedding solutio...
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