Method for preparing human body or animal accellular tissues
A decellularized tissue and animal tissue technology, applied in biochemical equipment and methods, tissue culture, prostheses, etc., can solve problems such as affecting product quality, tissue structure damage, long processing time, etc., to reduce procurement costs and production costs. , Short degreasing time, good product quality
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Embodiment 1
[0022] A method for preparing human or animal decellularized tissue, comprising the following steps:
[0023] (1) Primary tissue processing: Human or animal tissues (in this example: pleural peritoneum, esophagus mucosa, small intestine mucosa or pericardium tissue) are mechanically removed from the surface fat, and prepared into various clinically required tissues according to clinical purposes. kind of shape;
[0024] (2) Virus inactivation, degreasing and decellularization:
[0025] Proceed through the following steps in turn:
[0026] (1) Perform virus inactivation and degreasing treatment with the first inactivation and degreasing liquid for 30 minutes on the human body or animal tissue after primary processing; the first inactivation and degreasing liquid is composed of 2% peracetic acid, 1 Sodium chloride solution, 2% surfactant and the rest of water; use peracetic acid and sodium chloride solution to inactivate, and add surfactant to degrease at the same time, and ac...
Embodiment 2
[0036] A method for preparing human or animal decellularized tissue, comprising the following steps:
[0037] (1) Primary processing of tissues: removing surface fat from human or animal tissues (skin tissues) mechanically, and preparing them into various shapes required for clinical use;
[0038] (2) Virus inactivation, degreasing and decellularization:
[0039] Proceed through the following steps in turn:
[0040] (1) Use the first inactivation and degreasing solution to inactivate and degrease the virus for 10 minutes on the skin tissue of the human body or animal after initial processing; the first inactivation and degreasing solution is composed of 0.1% peracetic acid, 3% sodium chloride solution, 1% surfactant and the rest of water; use peracetic acid and sodium chloride solution to inactivate, add surfactant to degrease at the same time, and achieve virus inactivation The effect of degreasing has the characteristics of short degreasing time, high efficiency and strong...
Embodiment 3
[0050]A method for preparing human or animal decellularized tissue, comprising the following steps:
[0051] (1) Primary tissue processing: human or animal tissues (in this example, blood vessels, nerves, heart valves, bones, cartilage or tendon tissues) are mechanically removed from the surface fat, and prepared into various clinically required tissues according to clinical purposes. shape;
[0052] (2) Virus inactivation, degreasing and decellularization:
[0053] Proceed through the following steps in turn:
[0054] (1) The first inactivation and degreasing liquid is used for virus inactivation and degreasing for 20 minutes on the human body or animal tissue after primary processing; the first inactivation and degreasing liquid is composed of 1% peracetic acid, 5% sodium chloride solution, 0.5% surfactant and the rest of the water; use peracetic acid and sodium chloride solution to inactivate, add surfactant to degrease, and achieve degreasing while virus inactivation It...
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