Method for extracting protein from sesame

A protein and sesame technology, which is applied in protein food ingredients, protein composition of vegetable seeds, food science, etc., can solve the problems of using organic solvents, long separation cycle, and complicated process, and achieve mild separation conditions, strong water solubility, Low dosage effect

Active Publication Date: 2013-07-10
SHANDONG FANGJIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional extraction of sesame protein is usually separated by enzymatic method, alkaline method and chromatographic method. These methods have the disadvantages of long separation period, cumbersome process and low yield, and the use of organic solvents affects the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for extracting protein from sesame, its step is as follows:

[0018] 1. Weigh 100 grams of sesame seeds, grind them into fine powder through superfine grinding.

[0019] 2. Preparation of sesame protein extract: add 0.1mol / L of Na laurate containing 2% polyoxyethylene laurate to the sesame fine powder gained in step 1 2 HPO 4 1800ml of the aqueous solution was stirred and leached, and filtered after leaching for 1 hour to obtain the leaching solution of the sesame protein.

[0020] 3. Cloud point phase separation extraction: in two steps:

[0021] According to the solid-to-liquid ratio g / ml is 1:100, add lauric acid polyoxyethylene ether to the sesame protein extraction liquid obtained in step 2, fully mix to form a micellar solution, drop HCl solution in the micellar solution to adjust the pH value was 4.5, heated to a temperature of 80°C, and carried out phase-separation extraction to form a micellar phase and an aqueous phase, and centrifuged after 1 ho...

Embodiment 2

[0025] Except following difference, other is with embodiment 1.

[0026] 1. Weigh 100 grams of sesame seeds, grind them into fine powder through superfine grinding.

[0027] 2, preparation of sesame protein leaching solution: add the sodium laurate polyoxyethylene ether containing 4% in the sesame fine powder of step 1 gained 2 HPO 4 Aqueous solution 2000ml, carry out stirring leaching, filter after leaching for 2 hours, obtain the leaching solution of sesame protein.

[0028] 3. Cloud point phase separation extraction: in two steps:

[0029] Be 5:100 by solid-to-liquid ratio g / ml, add lauric acid polyoxyethylene ether in the sesame protein extraction liquid of step 2 gained, add lauric acid polyoxyethylene ether in the sesame protein extraction liquid of step 2 gained, Mix well to form a micellar solution, add 0.2mol / L HCL solution dropwise to the obtained micellar solution to adjust the pH to 4.6, heat to a temperature of 85°C, conduct phase separation extraction to form ...

Embodiment 3

[0033] Except following difference, other is with embodiment 1.

[0034] 1. Weigh 100 grams of black sesame seeds, grind them into fine powder through superfine grinding.

[0035] 2. Preparation of black sesame protein extract: add NaH containing 2% polyoxyethylene laurate to the black sesame fine powder obtained in step 1 2 PO 4 2000ml of aqueous solution was stirred and leached, filtered after leaching for 2 hours, and the leaching solution of black sesame protein was obtained.

[0036] 3. Cloud point phase separation extraction: in two steps:

[0037] According to the solid-to-liquid ratio g / ml is 3:100, add lauric acid polyoxyethylene ether to the sesamin extract solution obtained in step 2, fully mix to form a micellar solution, drop 0.1mol / ml in the obtained micellar solution Adjust the pH of the L HCL solution to 5.0 and heat it to a temperature of 85°C for phase-separation extraction to form a micelle phase and an aqueous phase, and centrifuge to separate the micell...

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PUM

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Abstract

The invention relates to a protein separation and purification method and particularly relates to a method for extracting protein from sesame. The method comprises the following steps of: performing ultrafine crushing on the sesame to form powder, preparing a sesame protein leaching solution, performing cloud point phase separation extraction, and performing dialysis purification to prepare the sesame protein. According to the method disclosed by the invention, a cloud point extraction method is utilized for extracting the protein, thus the using quantity of an organic solvent is low, the operation is simple, and the large-scale operation is facilitated; the nature of the sesame protein is fully utilized, the water solubility under basic conditions is strong, cloud point extraction is performed twice, and the sesame protein with few impurities and high purity is obtained; and the method further has the advantages of low using quantity of surfactant, low cost, simple system composition, high extraction rate, short preparation cycle and mild separation conditions.

Description

technical field [0001] The invention relates to a protein separation and purification method, in particular to a method for extracting protein from sesame. Background technique [0002] Sesame has high use value because it is rich in oil and protein. But for a long time the use of sesame is limited to the use of oil. Sesame meal, whose main component is protein after oil extraction, is mostly used as feed. The protein content of sesame protein can reach 50%. The amino acid composition of sesame protein is in good agreement with the human protein standard recommended by FAO HO. The essential amino acid content of sesame protein accounts for 30% of the total amino acid. Essential Amino Acids Except for lysine and isoleucine, which are lower than the requirements of FAO HO, other amino acids can meet the needs of the human body. The NPU of sesame protein is 56%. If Lys is added, the NPU value can be increased to 81%. Therefore, sesame protein can be used as an additive in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14
Inventor 杜新磊徐亚娟李海燕张国印
Owner SHANDONG FANGJIAN PHARMA
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