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High throughput screening method for anti-influenza virus inhibitor with PAC-PB1N complex as target

A technology of anti-influenza virus, screening method, applied in antiviral agents, medical preparations containing active ingredients, measuring devices, etc.

Inactive Publication Date: 2013-07-03
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the rapid mutation and evolution of influenza viruses, it is difficult for traditional vaccines to be targeted for future influenza epidemics, and the influenza virus itself continues to develop resistance to existing drugs. Therefore, the development of new anti-influenza drugs has become an extremely urgent major issues

Method used

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  • High throughput screening method for anti-influenza virus inhibitor with PAC-PB1N complex as target
  • High throughput screening method for anti-influenza virus inhibitor with PAC-PB1N complex as target
  • High throughput screening method for anti-influenza virus inhibitor with PAC-PB1N complex as target

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0088] The experiment of anti-influenza virus material screening method is as follows:

[0089] 1. Experimental design

[0090]ELISA is the abbreviation of enzyme-linked immunosorbent assay (enzyme linked immunosorbent assay). High test technology. The drug screening system of the present invention uses an experimental method similar to ELISA (see image 3 ). PA C Fragments were immobilized to the bottom of a 96-well ELISA plate by hydrophobic interaction, and unbound PA was blocked with bovine serum albumin (BSA) C The location of the fragment. Afterwards, a PB1-GST fusion protein solution containing an appropriate dilution concentration of the traditional Chinese medicine extract is added for incubation. Then add rabbit GST antibody, goat anti-rabbit GST antibody-horseradish peroxidase-coupled protein, add protein solution and incubate at each step, wash fully with buffer to remove unbound protein. Finally add the substrate TMB-H 2 o 2 Carry out the color reaction, ...

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Abstract

The invention provides a screening method for an anti-influenza virus material, and an application thereof, and more specifically provides a method for screening the anti-influenza virus material from natural product extracts based on an enzyme-linked immunoassay technique. The screening method can screen and obtain effective anti-influenza virus material from a great amount of the natural product extracts efficiently, conveniently and accurately, and can be used for preparing drugs capable of treating and / or preventing the influenza virus. The invention also relates to a high throughput screening method for an anti-influenza virus inhibitor with PAC-PB1N complex as a target.

Description

technical field [0001] The invention relates to a method for screening anti-influenza virus substances and its application, specifically through the system of enzyme-linked immunosorbent adsorption and high-throughput drug screening, the C-terminus of PA subunit of avian influenza virus (PA C Fragment) - PB1 subunit N-terminal (PB1 N Fragment) complex is used as the substrate to screen the anti-influenza virus activity of Chinese medicine extracts, and thus obtain active Chinese medicine extracts such as clove, geranium, xuejianchou or emblica. Background technique [0002] Influenza, referred to as influenza, is a common acute respiratory infectious disease caused by influenza virus. It is more common in winter and spring. It is clinically characterized by severe systemic poisoning symptoms such as high fever, fatigue, headache, and body aches, while other respiratory symptoms are mild. . Influenza viruses belong to the Orthomyxoviridae family and can be divided into thre...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N21/31A61K36/185A61K36/47A61K36/61A61P31/16
Inventor 饶子和杨诚李雪梅陈瑜涛贾艳茹刘影影
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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