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Microalgae grease extraction method

A technology of microalgae oil and extraction method, which is applied in the direction of fat oil/fat production and fat production, which can solve the problems of loss of biologically active components, high temperature, and long time, and achieve low cost, low energy consumption, and low energy consumption. Effect

Active Publication Date: 2013-06-26
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious shortcomings in the above methods, such as long time, high temperature, poor crushing effect, or no effective protection of biological active ingredients.
The result is inefficient cell wall disruption and significant loss of bioactive components
[0005] Patent 200810047859 discloses a method for producing docosahexaenoic acid oil by using biological enzyme method to break the wall. In this method, protease and non-protease are added at the same time and participate in enzymolysis reaction at the same time. Since non-protease itself is also protein, Therefore, in actual use, various proteases will also decompose and destroy other biological enzymes, resulting in a significant decrease in the breaking efficiency

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0036] Oil extraction using wet algae, a species of Chlorococcus. Design two experimental groups A and B, each with 3 parallel samples, take 30g of wet algae from each sample, and adjust the final concentration of wet algae to 100g / L according to the water content. Add dilute sulfuric acid to both groups A and B until the pH value is 2, seal them with sealing film, and treat them at 135°C and 0.2 MPa for 1 min; then add 0.1 g of acid cellulase, 0.02 g of acid pectinase, Acid protease 0.05g, 40°C, 120rpm oscillating for 12h; group B added 0.1g of acid cellulase, acid pectinase 0.02g, 40°C, 120rpm oscillating for 6h, then added acidic protease 0.05g, 40°C, Continue to shake and vortex at 120rpm for 6h.

[0037] Put each algae sample in a 500mL conical flask, add n-hexane at a ratio of 1:10 (algae dry weight / solvent, DWg / mL), fully stir and extract in a water bath at 50°C for 20min, centrifuge at 3000rpm for 3min, and transfer the supernatant ; Repeat this step three times. Th...

Embodiment 2

[0042] Oil extraction was carried out with wet algae, a type of Scenedesmus. Design four experimental groups as shown in Table 2, each with 3 parallel samples, take 30 g of wet algae from each sample, and adjust the final concentration of wet algae to 100 g / L according to the water content.

[0043] Table 2

[0044] Experimental group number

Treatment of experimental group

A’

none

B’

pH=2, enzyme treatment

C’

High temperature and high pressure, pH=2

D’

High temperature and high pressure, pH=2; enzyme treatment

[0045] Group A' is the control group without any treatment; groups C' and D' add dilute sulfuric acid until the pH value is 2, seal them with sealing film, and treat them at 135°C and 0.2MPa for 1 min; group B' add dilute sulfuric acid until the pH value is 2. The pH value is 2, add 0.1g of acid cellulase and 0.02g of acid pectinase to the cooled D' group, shake and mix at 40°C and 120rpm for 6h,...

Embodiment 3

[0051] Oil extraction was performed using wet algae, a species of Scenedesmus. Design two experimental groups A and B, each with 3 parallel samples, take 30g of wet algae from each sample, and adjust the final concentration of wet algae to 100g / L according to the water content. Group A is the control group without any treatment; Group B is added with dilute sulfuric acid until the pH value is 1.5, sealed with bottle sealing film, and treated at 150°C and 0.1MPa for 120min; then add 0.1g of acid cellulase and acid pectinase 0.02g, 0.05g acid protease, shake and vortex at 30°C and 30rpm for 48h.

[0052] Put each algae sample in a 500mL conical flask, add n-hexane at a ratio of 1:10 (algae dry weight / solvent, DWg / mL), fully stir and extract in a water bath at 50°C for 20min, centrifuge at 3000rpm for 3min, and transfer the supernatant ; Repeat this step three times. The supernatants were combined, filtered through filter paper, and the solvent was distilled off with a rotary e...

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Abstract

The invention provides a microalgae grease extraction method which comprises the following steps: regulating the pH value of a microalgae solution, heating, pressurizing, and then returning to the normal pressure state; and adding biological enzymes, and mixing, wherein the biological enzymes are added in two steps, non-proteinase is firstly added, and proteinase is secondly added. According to the method provided by the invention, when the biological enzymes are used for treating wet algae, the proteinase is added after the non-proteinase acts for some time, so that the non-proteinase can not be decomposed or destroyed by the proteinase, thereby greatly increasing the wall-breaking efficiency. Compared with the method of simultaneously adding the proteinase and the non-proteinase, the wall-breaking efficiency is obviously increased, thereby obviously increasing the grease extraction rate and yield.

Description

technical field [0001] The invention relates to a processing method of microalgae, in particular, the present invention relates to a method for extracting oil from microalgae. Background technique [0002] Microalgae are a class of individual tiny photoautotrophic and / or heterotrophic single-celled organisms, which have the characteristics of wide distribution, various species, high photosynthetic efficiency, fast growth rate, and strong adaptability. Annual CO fixation by microalgae 2 It accounts for about 40% of the global net photosynthetic output. In addition, it is rich in lipids, hydrocarbons, proteins, soluble polysaccharides, and high-value natural pigments such as astaxanthin and β-carotene. Therefore, it is important in environmental protection, energy and health. Today, when the problem is attracting much attention, microalgae are getting more and more attention. [0003] After the microalgae culture is completed, it is necessary to collect, dry, break and extra...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/02C11B1/10
Inventor 马欣欣刘敏胜石蕾杨巧利杜彦山
Owner ENN SCI & TECH DEV
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