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Microalgal wall-breaking method

A technology of microalgae and algae oil, which is applied to the dissolution of microorganisms, fat production, fat oil/fat production, etc., which can solve the problems of large external operating conditions, long processing time, and difficulty in achieving scale-up, and achieve easy realization Effects of scale-up, saving cost and energy consumption, and eliminating drying steps

Inactive Publication Date: 2013-06-19
ENN SCI & TECH DEV
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AI Technical Summary

Problems solved by technology

This method has the following disadvantages: 1) the concentration of algae liquid is low (about 0.1g / L in terms of dry weight); It is destroyed, so it needs to be carried out under ice bath, which has great requirements on external operating conditions; 3) It has high requirements on the characteristics of algae itself, and is only suitable for microalgae with thinner cell walls; 4) For microalgae with slightly thicker cell walls, the treatment It takes a long time, so the energy consumption is very high during industrial scale-up, and it is difficult to achieve large-scale scale-up
[0006] At present, in the field of microalgae processing, there is no relevant report on the use of ozone to break the wall of microalgae to extract microalgae oil

Method used

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Embodiment 1

[0026] For the application in the oil extraction of Scenedesmus obliquus, Scenedesmus obliquus was purchased from FACHB-12, Institute of Hydrobiology, Chinese Academy of Sciences. For detailed information such as the cultivation of this algae species, refer to "Ecological Environment" 2006, 15( 5), "Effects of Reed Allelochemical Components on Growth Characteristics of Scenedesmus obliquus", 925-929.

[0027] Put 4L of the oil-enriched Algae concentrated solution that has been cultivated until the fatty acid content is 51.6%, and the cell concentration is 65g / L in a sealed extraction tank, put the probe of the ultrasonic processor into the extraction tank, and feed ozone with a concentration of 1500g. 3 / m 3 , use 400w ultrasonic power, ultrasonic frequency is 20-25kHz, intermittent ratio 3 / 5 (s / s) treatment, the total treatment time is 15min, centrifuge to collect algae, separate the supernatant liquid and release the supernatant water-oil mixture to separate liquid, The upp...

Embodiment 2

[0030] The application on Nannochloropsis oculata (nannochloropsis oculata), which was purchased from C1502 of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences. For details such as the cultivation of the algae, see "China Marine Medicine", 2003, 91 (1), "Large-scale cultivation of EPA-rich marine microalgae Nannococcus ophthalmica", 5-10.

[0031] Put 4L of Nannochloropsis eyepoint concentrate solution cultivated to a fatty acid content of 50.3% and a cell concentration of 67g / L into a sealed extraction tank, put the probe of an ultrasonic processor into the extraction tank, and inject ozone to increase the concentration. 1720gO 3 / m 3 , use 400w ultrasonic power, ultrasonic frequency is 20-25kHz, intermittent ratio 3 / 5 (s / s) treatment, total ultrasonic time is 15min, centrifuge to collect algae, separate the supernatant liquid and release the supernatant water-oil mixture to separate liquid, The upper oil sample was taken, dehydrated, a...

Embodiment 3

[0040] Schizochytrium sp. was purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee, and its number is CCTCC No.M209059. For detailed information on the cultivation method of this algae, please refer to Chinese patent application number: 200910033869.5;

[0041] Put 4L of Schizochytrium concentrated solution cultivated to a fatty acid content of 52% and a cell concentration of 120g / L into a sealed extraction tank, put the ultrasonic processor probe into the extraction tank, and inject ozone with a concentration of 90gO 3 / m 3 , use 400w ultrasonic power, ultrasonic frequency is 20-25kHz, intermittent ratio 3 / 5 (s / s) treatment, total ultrasonic time is 8min, centrifuge to collect algae, release the upper layer of water-oil mixture for static separation, take the upper layer of oil Sample, dehydration, filters to obtain algal hair oil, calculates the oil extraction rate according to the method that embodiment 1 provides,...

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Abstract

The invention provides a microalgal wall-breaking method, which includes: adding microalgae into a sealed container, then introducing ozone into the sealed container, and simultaneously carrying out an ultrasonic treatment on the microalgae. The method has the advantages of high wall breaking efficiency, oil extraction rate up to more than 80%, or even to over 90%, low energy consumption, simple process, and easy realization of large-scale enlargement, thus being suitable for industrialized application.

Description

technical field [0001] The present invention relates to the processing method of microalgae, specifically, the present invention relates to the breaking method of microalgae. Background technique [0002] Microalgae has the characteristics of high photosynthetic efficiency, strong environmental adaptability, short growth cycle, high biological output, and high oil content in some species. Therefore, microalgae is a good material for preparing bioenergy, and has attracted widespread attention from all over the world. There has been an upsurge in research on microalgae as a raw material for biomass energy worldwide. [0003] At present, the high lipid microalgae found in the world have the characteristics of small cells (2-20 μm) and thick cell walls, which increase the difficulty of oil extraction from microalgae. In addition, the cell walls of oily microalgae are generally composed of cellulose or silicon. The structure is firm and has certain oxidation resistance. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C11B1/00
Inventor 杨巧利邵元龙杜彦山
Owner ENN SCI & TECH DEV
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