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Mixed liquid fermentation process of three plants of bacillus licheniformis

A technology of Bacillus licheniformis and liquid fermentation, which is applied in the direction of bacteria, mutant preparation, and treatment of microorganisms with electricity/wave energy. Production cost, the effect of optimizing the fermentation process

Inactive Publication Date: 2013-06-12
SHANXI KINGSHINE FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this fermentation method, the production cost is high, and affected by the fermentation process, the performance of the product produced in batches is not stable

Method used

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  • Mixed liquid fermentation process of three plants of bacillus licheniformis
  • Mixed liquid fermentation process of three plants of bacillus licheniformis
  • Mixed liquid fermentation process of three plants of bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 strain screening

[0026] 1. Culture on solid slope

[0027] Three strains of Bacillus licheniformis L1, L2, and L3 were inoculated on the slant medium under aseptic conditions for cultivation, among which: the cultivation condition of Bacillus licheniformis L1 was 37°C for 24 hours, and the cultivation condition of Bacillus licheniformis L2 was 30°C. ℃, 24h, the culture condition of Bacillus licheniformis L3 is 32℃, 24h;

[0028] In terms of weight percentage, the components of the slant medium used are: 0.5% glucose, 0.5% sucrose, 1.0% yeast powder, 0.5% beef extract, 0.5% tryptone, 0.1% diammonium hydrogen phosphate, 0.5% sodium chloride, Manganese sulfate 0.005%, ammonium sulfate 0.5%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.1%, agar 1.5%, the balance is water, and the pH is 7.2.

[0029] 2. Physical and chemical factor mutagenesis

[0030] Take the above-mentioned solid slant culture, and prepare bacterial suspension with 0.9% physiolo...

Embodiment 2-4

[0055] Embodiment 2-4 mixed fermentation experiment

Embodiment 2

[0057] Under aseptic conditions, take the single colony slant (mother slant) of L1-13, L2-18, and L3-17 out of the refrigerator at 4 °C, wipe the outer wall with 75% alcohol cotton ball, and bring it into the sterile room. On the ultra-clean workbench, use a sterile inoculation shovel to scrape 0.1cm from the mother slope 2 Quickly insert the bacteria lawn into the sterile slant medium, and spread it evenly in an "S" line from top to bottom. After bandaging, culture at 30°C for 48 hours to obtain second-generation seed slopes of L1-13, L2-18, and L3-17, numbered L1-13F2, L2-18F2, and L3-17F2, and store them in a refrigerator at 4°C after the cultivation . In terms of percentage by weight, the components of the slant medium used are: 0.2% glucose, 0.5% sucrose, 0.5% yeast powder, 0.9% beef extract, 0.8% tryptone, 0.1% diammonium hydrogen phosphate, 0.5% sodium chloride, Manganese sulfate 0.005%, ammonium sulfate 0.5%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.1...

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Abstract

The invention discloses a mixed liquid fermentation process of three plants of bacillus licheniformis. According to the mixed liquid fermentation process, the three plants of the bacillus licheniformis, namely L1, L2 and L3 with different physiological functions, which are originally cultured under different conditions, are subjected to solid culture and shaking flask seed preparation by performing artificial mutation breeding and optimizing process formula and culture conditions, then shaking flask seed liquid of the three plants of the bacillus licheniformis is merged into a flask, first-stage seed liquid preparation and second-stage seed liquid preparation are performed, and fermentation culture is finally performed in a same fermentation tank. By adopting the mixed fermentation process disclosed by the invention, the bacteriostatic ability is improved by 10-15%, the sporation rate is improved by 16-23% and the cost is saved by 200%.

Description

technical field [0001] The invention relates to a three-strain bacillus licheniformis mixed liquid fermentation process, which belongs to the field of microbial fermentation. Background technique [0002] Bacillus licheniformis, as a kind of Bacillus genus, can promote the body to produce antibacterial active substances, kill pathogenic bacteria, produce antibacterial active substances, and has a unique mechanism of biological oxygen capture, which can inhibit the growth and reproduction of pathogenic bacteria. Widely used in medicine, pesticide, food, feed processing, environmental pollution and other industries. Bacillus licheniformis is a new micro-ecological preparation developed and utilized in my country since the 1990s, and its growth and decline directly affect the efficacy and yield of the preparation. At present, the production method of Bacillus licheniformis can be divided into solid fermentation and liquid fermentation according to the fermentation method. But...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12N13/00C12R1/10
Inventor 张晓红刘姝静朱慧平张中亲
Owner SHANXI KINGSHINE FERTILIZER
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