Full-spectrum super-resolution measuring method and label-free biomolecule measurement system

Abstract

The invention relates to a full-spectrum super-resolution measuring method and a label-free biomolecule measurement system. The method and the system are characterized in that membrane layers with different thicknesses ranging from 300nm to 4500nm are arranged on a chip and used for encoding, then sample application is carried out on the chip by means of probe protein to form a biological chip, the probe protein of the biological chip is fully combined with a to-be-tested sample, a to-be-tested object in the to-be-tested sample is arranged on the probe protein through specific adsorption, light beams with light intensity of I0 are vertically cast on the biological chip, when the light beams are vertically cast on the biological chip only covered with the membrane layers, different reflection spectrums are produced due to the different thicknesses of the membrane layers, thickness encoding of the biological chip is achieved, by means of an interference extremum method, the thicknesses t of the membrane layers of the biological chip are decoded according to wavelengths of extreme points in a light spectrum, when the light beams are cast on the to-be-tested object combined with the probe protein, thickness changes of the membrane layers in the range delta occur, intensity amplitude differences are obtained through calculation, and the thickness of the to-be-tested object is obtained. The method and the system can be widely used in thickness measurement of biomolecules.

Description

technical field [0001] The invention relates to a biomolecular thickness measurement method and a measurement system, in particular to a full-spectrum super-resolution measurement method and a non-labeled biomolecular measurement system. Background technique [0002] Due to its extremely high sensitivity and accuracy, the interference principle has been widely used in the precise measurement of film thickness. The earliest interferometer was jointly developed by American physicists Michelson and Morley in 1883, named Michelson interferometer. In the 1990s, with the maturity of microfabrication and digital imaging technology, white light interferometers began to appear. Unlike traditional Michelson interferometers which are only used to measure displacement, white light interferometers are mainly used to measure film thickness. The measurement arm and the reference arm of the white light interferometer are on the same side of the beam splitter, so the difference in length i...

AI Technical Summary

Problems solved by technology

At present, some studies have used interferometers to detect the reaction results of organic molecules in real time and without labels, but the harsh working conditions of white light interferometers limit the application of this instrument in the field of point-of-care testing (POCT).

In addition, there are other non-labeled biomolecular detection methods, such as surface plasmon resonance (SPR) method, electrical impedance method, etc., but these methods have encountered problems such as high cost of consumables, complicated instruments, etc., and cannot be used in real-time detection in the short term Domain (POCT) gets applied

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