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Eosino-thiobacillus and application thereof to copper pyrites leaching

A technology of Thiobacillus acidophilus and chalcopyrite, applied in the directions of bacteria, process efficiency improvement, microorganism-based methods, etc., can solve the problems of reducing bacterial concentration, reducing leaching efficiency, affecting leaching efficiency, etc., to reduce generation and high efficiency. The effect of using

Active Publication Date: 2013-06-05
JIANGNAN UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Although it has been reported in China in recent years that optimizing the bacterial community structure can further improve the leaching efficiency of chalcopyrite, due to the presence of a large amount of iron ions in the leaching system, a large amount of jarosite will inevitably be produced during the leaching process. The denseness of the precipitate is very good, and it will be tightly wrapped on the mineral surface, which prevents the contact between microorganisms and the mineral surface, and affects the leaching efficiency
Recent studies have pointed out that controlling the lower pH (<1.5) will reduce the formation of jarosite precipitation, but the optimum pH of common leaching bacteria, such as Thiobacillus ferrooxidans, is 1.8-2.5, and too high acid will reduce Bacterial concentration during leaching, thereby reducing leaching efficiency

Method used

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  • Eosino-thiobacillus and application thereof to copper pyrites leaching
  • Eosino-thiobacillus and application thereof to copper pyrites leaching
  • Eosino-thiobacillus and application thereof to copper pyrites leaching

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1 strain screening

[0018] In this example, 100mL of the wet leaching heap of the Zijinshan Copper Mine in Shanghang County, Fujian Province was taken, and the large particles were first filtered with filter paper, and then the filtrate was added to a 250ml Erlenmeyer flask containing 1% chalcopyrite, at 30°C, 170r / h min, carry out enrichment culture for 4 days, repeat 3-4 times, use Starky-Na 2 S 2 o 3 ·5H 2 O medium for plate separation, after 2 weeks, colonies are formed, and single bacteria are picked and dropped into Starky-S 0 The culture medium was cultured, and the plate separation was repeated 4 times to obtain pure bacteria.

Embodiment 2

[0019] Example 2 strain identification

[0020] The entire genome of the pure culture was extracted using the Bacterial Whole Genome Rapid Extraction Kit, and PCR was performed with 16S rDNA universal primers to identify the strain as Thiobacillus acidophilus.

Embodiment 3

[0021] Physiology, biochemistry and resistance research of the bacterial strain of embodiment 3

[0022] The morphology of the above-mentioned ZJJN was observed by transmission electron microscopy as follows: figure 1 . Take 10°C, 20°C, 25°C, 30°C and 45°C for the culture of the strains screened to determine the optimum growth temperature is 30°C; take initial pH 0.5, 0.8, 1.0, 1.5, 2.0 and 2.5 at the optimum The culture is carried out at a temperature, and the optimum growth pH range is determined to be 0.5-1.5. Determine the optimal energy substrate (g / l) S for the strain ZJJN by the following combination 0 10.0g, Na 2 S 2 o 3 ·5H 2 O10.0; FeSO 4 ·7H 2 O10.0; FeCl 2 10.0; Peptone 10.0; Yeast Extract 10.0; Glucose 10.0; Fructose, 10.0;

[0023] Using several antibiotics with a concentration gradient of 10 and 100mg / l, it was found that the sensitivity order of ZJJN to several common antibiotics was tetracycline > streptomycin > erythromycin > chloramphenicol > kanam...

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Abstract

The invention discloses extreme eosino-thiobacillus which is named Acidithiobacillus sp.ZJJN, and the preservation number of the eosino-thiobacillus is CCTCC NO: M2012104. The eosino-thiobacillus is highly sensitive to a plurality of kinds of antibiotics and highly tolerant to a plurality of types of common metal in a bio-leaching process. Moreover, the eosino-thiobacillus is used for assisting ferrous oxide thiobacillus in a chalcopyrite leaching experiment under an extreme potential of Hydrogen (pH), and a recovery rate of copper is found to be greatly improved compared with a contrasting system. A scanning electron microscope is used for observation of the surfaces of leached chalcopyrite, jarosites are found to be greatly reduced on the surfaces of the chalcopyrites in a system which is added with the strain ZJJN system, and leaching efficiency is greatly improved. The extreme eosino-thiobacillus can endure an environment with a quite low pH value, is quite suitable for a chalcopyrite leaching system, and is applied to industrial chalcopyrite leaching hopefully.

Description

technical field [0001] The invention relates to a strain of acidophilic thiobacillus, in particular to a strain of extreme acidophilic thiobacterium and its application. Background technique [0002] Bioleaching is an interdisciplinary subject in the field of biology and metallurgy, also known as biooxidation or biohydrometallurgy, that is, using microorganisms, air and water and other natural substances to leach lean ore, waste ore, tailings and smelting slag, etc., to recycle Certain precious non-ferrous metals and rare metals are a metallurgical method to prevent the loss of mineral resources and maximize the use of mineral deposits; compared with traditional smelting methods, bioleaching usually has less infrastructure investment, low operating costs, and less environmental pollution. Lower-grade minerals can be processed, so it is considered a green metallurgical technology. [0003] Primary sulfide copper ore chalcopyrite is the most stable and difficult copper minera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/234Y02P10/20
Inventor 杨海麟辛瑜冯守帅张玲
Owner JIANGNAN UNIV
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