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Food-grade high-content lutein ester and preparation method thereof

A technology for lutein ester and food grade is applied in the field of separating and preparing food grade high-content lutein ester, which can solve the problems of long process time, low yield and high energy consumption, and achieves simple process steps and low solvent residue. , the effect of short time

Active Publication Date: 2015-07-22
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many types of organic solvents involved in the patent, and the process takes a long time, consumes a lot of energy, and has a low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 20.0g of marigold extract with lutein ester UV content (UV detection content) of 31.2%, put it into a 250mL three-necked flask, add 80mL propanol, stir at 30°C for 4 hours, and filter to obtain filter cake I; filter cake Add 30mL of methanol to I, stir at 40°C for 1 hour and then filter to obtain filter cake II; filter cake II is dried at 30°C for 2.5 hours to obtain 4.7g of lutein ester product, wherein the UV content of lutein ester is 82.1%, The content of all-trans lutein ester determined by HPLC is 92.1%, and the n-hexane dissolved residue in the product is 0.3ppm.

[0036] The lutein ester product prepared in this example is an orange-red powder crystal with good looseness and fluidity.

Embodiment 2

[0038] Weigh 20.0g of marigold extract with lutein ester UV content of 29.5%, put it into a 250mL three-neck flask, add 100mL of recovered propanol once, stir at 40°C for 3 hours, filter to obtain filter cake I; add 40mL of Ethanol, stirred at 40°C for 0.5 hours and then filtered to obtain filter cake II; filter cake II was dried at 50°C for 1 hour to obtain 4.5g of lutein ester product, wherein the UV content of lutein ester was 84.4%, which was determined by HPLC The content of all-trans lutein ester is 92.0%, and the n-hexane dissolved residue in the product is 0.5ppm.

[0039] Described primary recovery propanol is the propanol that extracts marigold extract with the method of embodiment 1, the propanol that obtains through recovery.

[0040] The lutein ester product prepared in this example is an orange-red powder crystal with good looseness and fluidity.

Embodiment 3

[0042] Weigh 20.0g of marigold extract with lutein ester UV content of 29.5%, put it into a 250mL three-neck flask, add 120mL of n-butanol, and filter it after ultrasonic treatment at 35°C for 1 hour to obtain filter cake I; add 30mL of Ethanol, stirred at 30°C for 1 hour and filtered to obtain filter cake II; filter cake II was dried at 40°C for 2 hours to obtain 4.2 g of lutein ester product, wherein the UV content of lutein ester was 87.6%, and the crystal content was determined by HPLC. The content of all-trans lutein ester is 93.0%, and the residue in n-hexane is 0.3ppm.

[0043] The lutein ester product prepared in this example is an orange-red powder crystal with good looseness and fluidity.

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PUM

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Abstract

The invention provides a food-grade high-content lutein ester and a preparation method thereof. The preparation method comprises the following steps: adding C2-4 alcohol solvent into a marigold extractum, stirring and washing for 3-6 hours or carrying out ultrasonic treatment for 1-2 hours at 30-60 DEG C, cooling to 30-50 DEG C, and filtering to obtain a filter cake I; adding methanol or ethanol into the filter cake I to wash the filter cake I 1-3 times, and filtering to obtain a filter cake II; and drying the filter cake II to remove the solvent, thereby obtaining the high-content lutein ester crystal. The lutein ester content in the lutein ester product obtained by the method is at least 80%, wherein the all-trans lutein ester content is at least 90%; and the n-hexane solvent residue is less than 1.0 ppm. The method provided by the invention has the advantages of simple steps, environmental protection, high product content, low solvent residue, short time consumption and low cost, can directly reutilize the recovered solvent, and can easily implement industrial production.

Description

technical field [0001] The invention belongs to the technical field of natural pigment preparation, and in particular relates to a method for separating and preparing food-grade high-content lutein ester from marigold extract. Background technique [0002] Lutein esters widely exist in plants in nature. Lutein esters in edible fruits and vegetables enter the human body through diet and are hydrolyzed into lutein monomers, which are absorbed and utilized by the human body. Studies have shown that lutein has many functions such as coloring, anti-oxidation, eye protection, anti-cardiovascular disease and cancer, and is widely used in food, medicine, eye health products, cosmetics and aquaculture. Studies have found that lutein esters have better bioavailability than lutein monomers, and were approved as new resource foods by the Ministry of Health in 2008 in the No. Residual requirement n-hexane <10ppm), so lutein ester products have been emerging in the market in recent ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 吴文忠薛凌云徐维锋赵林英张显仁
Owner INNOBIO CORP LTD
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