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Cultivating method of laying hen with low egg yolk cholesterol content

A breeding method, cholesterol technology, applied in the field of poultry genetics and breeding, can solve the problems of laborious, expensive, slow breeding progress, etc., to achieve the effect of reducing feeding costs, reducing the amount of feeding, and improving the accuracy of selection

Active Publication Date: 2014-11-05
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with conventional breeding technology, due to the long period of time, the breeding progress is slow, laborious and expensive; the use of feed nutrition regulation, although the effect is obvious, the problem of additives or drug residues and the impact on production performance and environmental sanitation cannot be ignored.

Method used

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  • Cultivating method of laying hen with low egg yolk cholesterol content
  • Cultivating method of laying hen with low egg yolk cholesterol content
  • Cultivating method of laying hen with low egg yolk cholesterol content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening of selected chicken samples

[0026] 1. Extraction of sample chicken DNA

[0027] In the breeding base group, 500 sample chickens were selected to be reared in single cages, and the DNA of the sample chickens was extracted. The steps are as follows:

[0028] 1) DNA extraction: Add 500 μL TE buffer, 12 μL SDS lysate (25%), and 10 μL proteinase K (10 mg / L) to 80 μL whole blood, shake and mix for 30 minutes, digest in a water bath at 57 °C overnight; add Tris saturated phenol 602μL, shake well for 10min, centrifuge at 10,000r / min for 10min; take the supernatant, add 500μL of Tris-saturated phenol, shake for 10min, and centrifuge at 10,000r / min for 10min; take the supernatant, add an equal volume of phenol / chloroform / iso Pentanol (25:24:1), shake slowly for 10min, centrifuge at 1,2000r / min for 10min; take the supernatant, add an equal volume of chloroform / isoamyl alcohol (24:1), shake slowly for 10min, 10000r / min / min and centrifuge for 10min; take the supernata...

Embodiment 2

[0061] The sample chickens of selected genotypes in Example 1 were selected for pure line selection by the closed group breeding method, and 50 families were established, with a male-to-female ratio of 1:10. Individual cages were raised to 40 weeks of age, and the production rate of each hen was recorded. Egg number, average egg weight and egg yolk cholesterol content at the age of 40 weeks, calculated the mean value of egg number, egg weight and egg yolk cholesterol content of all hens in 50 families and hens in each family, as shown in Table 5. Taking egg yolk cholesterol content as the main trait, taking egg weight and egg production into consideration, selecting the top 15 families whose egg yolk cholesterol content is lower than the group average, and egg weight and egg production higher than the group average, to breed the next generation, see Table 6 .

[0062] Table 550 families of all hens and the average value of the three traits of egg number, egg weight and egg yol...

Embodiment 3

[0069] Extract the DNA of the 2-week-old offspring of the family chickens selected in Example 2, and carry out PCR-RFLP analysis with a pair of primers in Example 1. According to the VLDLR gene molecular marker g.10428T>C of the candidate individual, select the TT type to carry out early Molecular marker-assisted selection.

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Abstract

The invention discloses a cultivating method of a laying hen with low egg yolk cholesterol content. The cultivating method of the laying hen with the low egg yolk cholesterol content comprises the following steps: 1, using deoxyribonucleic acid (DNA) sequence containing very low density lipoprotein receptor (VLDLR) gene of the laying hen as a template, designing a primer, and using polymerase chain reaction(PCR) to restriction fragment length polymorphism (RELP) method to conduct sample hen individual genotyping; 2, using a gene effect analysis model to determine select and remain genotype; 3, establishing a family of cock and hen individuals conforming to the select and remain genotype; 4, extracting DNA of offspring of hens of a select and remain genotype family, using the primer in the step 2 to conduct PCR-RFLP analysis, and selecting and remaining hens of the select and remain genotype in the step 2; 5, repeating the step 3 and the step 4 subculture and seed selection at least three generations, and obtaining laying hens with the low egg yolk cholesterol content. The cultivating method of the laying hen with the low egg yolk cholesterol content adopts a VLDLR gene molecular marker assisted select breeding method, egg yolk cholesterol contents of the cultivated laying hens can decrease more than 20 percentages compared with the original level.

Description

technical field [0001] The invention relates to a method for cultivating laying hens with low egg yolk cholesterol content, and belongs to the technical field of poultry genetics and breeding. Background technique [0002] Cholesterol is an important part of various membrane phase structures of animal cells and nerve myelin sheath. It is the precursor of bile acids, steroid hormones and vitamin D3, and has important physiological functions. However, if the body takes in a large amount of cholesterol, its content in the blood will increase significantly, and this is one of the important factors that cause cardiovascular diseases such as coronary arteriosclerosis and cerebral thrombosis. The American Heart Association recommended in the early 1970s that cholesterol intake should be less than 300 mg per day. Dietary cholesterol comes from animal foods, and eggs are one of the higher cholesterol content in animal foods. Studies have shown that cholesterol in eggs is an importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 田亚东康相涛刘向波魏杨韩瑞丽孙桂荣李国喜
Owner HENAN AGRICULTURAL UNIVERSITY
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