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Culture medium for germinating boletus spore

A technology for spore germination and culture medium, which can be used in applications, fertilizer mixtures, gardening, etc. It can solve the problems of different spore germination, and achieve the effect of neat colony shape, simple formula and strong operability.

Active Publication Date: 2013-05-22
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The spores of edible fungi are important reproductive materials, but the germination of the spores varies according to the category to which they belong. There are few reports on the spores of Boletus punctatus

Method used

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  • Culture medium for germinating boletus spore
  • Culture medium for germinating boletus spore
  • Culture medium for germinating boletus spore

Examples

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preparation example Construction

[0048] The preparation of PDA medium is within the skill of those skilled in the art. For example, put 50g of blood red rivet mushroom dry product into 1000ml of water, boil for about 30 minutes (for example, 25 minutes to 35 minutes), filter to obtain the blood red rivet mushroom boiling liquid, use this boiling liquid 1000ml, put in small potato pieces (soy bean grains) Size small pieces, such as 3mm to 5mmX3mm to 5mmX3mm to 5mm small pieces, such as 4mmX4mmX4mm small pieces) 200g, boil for about 5 minutes (for example, 4 minutes to 6 minutes), filter and discard the potato residue, add 20g of agar and 20g of glucose , supplemented with water to 1000ml, boiled to obtain blood red rivet mushroom boiling liquid+PDA medium (ie the medium of the present invention, for example, compiled as M2 medium in the examples).

[0049] In some preferred embodiments, the method further includes a sterilization treatment step of sterilizing the culture obtained from the cooking step of the r...

Embodiment

[0058] The present invention will be further described below through examples, but these examples are for illustration purposes only and should not be construed as limiting the scope of the present invention.

[0059] The preparations used in the present invention were all purchased from Beijing Chemical Reagent Company.

[0060] Collection of Spores of Boletus punctatus

[0061] Take a 250ml wide-mouth glass bottle and several bottle caps one day before the collection, lay a piece of round filter paper on the bottom of the bottle, and seal the cap; prepare several sheets of 20cm X20cm long and wide kraft paper or newspaper, several rubber bands, and sterilized blades 5- 10 pcs, scissors and tweezers. The above-mentioned items were autoclaved at 121°C for 30 minutes, and placed in a plastic normal temperature box sterilized with alcohol surface for later use. Also prepare 1-2 incubators for surface alcohol disinfection. All utensils are commercially available products, wi...

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Abstract

The invention provides a culture medium for germinating boletus spores, and the culture medium comprises a dried chroogomphus rutilus product, a PDA (Potato Dextrose Agar) culture medium and water. The invention also provides a method for preparing the culture medium and a method for germinating the boletus spores by adopting the culture medium. The culture medium provided by the invention has the advantages of simple formula, easiness for preparation, more spore germination, regular colonial morphology, thick and dense hypha, and the like.

Description

technical field [0001] The invention relates to a method for cultivating mycorrhizal edible fungus spores, in particular to a medium for germinating boletus spores and a method for cultivating boletus mycelia using the medium. Background technique [0002] Boletus belongs to the family Boletaceae (Boletaceae) in the Basidiomycotina Agaricaceae. Except for a few varieties that are poisonous or bitter and cannot be eaten, most of the varieties are edible. There are mainly white, yellow and black boletus. White boletus is delicious and nutritious. The fungus has a large body, thick flesh, strong stalk, sweet and delicious taste, and rich nutrition. It is a world-renowned edible fungus. People of all ethnic groups in Yunnan Province like to collect fresh mushrooms for cooking. Western European countries also have the habit of eating white boletus widely. In addition to fresh cooking, most of them are sliced ​​and dried, processed into various small packages, used to prepare s...

Claims

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Application Information

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IPC IPC(8): C05G1/00A01G1/04
Inventor 陈青君牛玉蓉张国庆程继鸿王明花杨瑞
Owner BEIJING UNIV OF AGRI
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