Chitosan nano particle, biological pesticide preparation for controlling red fire ants and preparation method thereof
A technology of chitosan nanoparticles and biopesticides, which is applied in the fields of botany equipment and methods, biocides, animal repellants, etc., and can solve the physical and chemical indicators of unrealized standardization level, nanoparticle size distribution, and drug loading rate Instability, cumbersome process and other problems, to achieve the effect of industrialized large-scale operation, high pest control effect, and breakthrough operation scale
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Embodiment 1
[0048] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation using the double-stranded ribonucleic acid sequence of the e-chain gene of the red fire ant ATP synthase as the active ingredient.
[0049] (1) Design and synthesis of double-stranded ribonucleic acid technical components for ATP synthase e-chain gene of RIFA GenBank: GU471223.1
[0050] Si-ATP_001: TCATC CGCGA GCAAG AT
[0051] Sense strand (5'-3'): 5'UCAUC CGCGA GCAAG AU dTdT 3' SEQ ID NO. 1
[0052] Antisense strand (3'-5'): 3'dTdT AGUAG GCGCU CGUUC UA 5'SEQ ID NO.2
[0053] Si-ATP_002: GTACGTGACG CCAAG CT
[0054] Sense strand (5'-3'): 5' GUACG UGACG CCAAG CU dTdT 3' SEQ ID NO.3
[0055] Antisense strand (3'-5'): 3'dTdT CAUGC ACUGC GGUUC GA 5'SEQ ID NO.4
[0056] Si-ATP_003: GTGCT GTTTC AACAT CA
[0057] Sense strand (5'-3'): 5' GUGCU GUUUC AACAU CA dTdT 3' SEQ ID NO. 5
[0058] Antisense strand (3'-5'): 3'dTdT CACGA CAAAG UUGUA GU 5'SEQ I...
Embodiment 2
[0079] This example particularly provides preparation and effect research of chitosan nanoparticle biological preparation with double-stranded ribonucleic acid sequence of red fire ant glutathione transferase gene as an active ingredient.
[0080] (1) Design and synthesis of double-stranded ribonucleic acid technical components for RIFA glutathione transferase gene GenBank: AY255670.1
[0081] Si-GSTS1_001: TAGAC TGCAA GAACA TG
[0082] Sense strand (5'-3'): 5'UAGAC UGCAA GAACA UG dTdT 3'SEQ ID NO.7
[0083] Antisense strand (3'-5'): 3'dTdT AUCUG ACGUU CUUGU AC 5'SEQ ID NO.8
[0084] Si-GSTS1_002: CGGTG GTTAC TTTGT CG
[0085] Sense strand (5'-3'): 5'CGGGUGGUUAC UUUGUCG dTdT 3'SEQ ID NO.9
[0086] Antisense strand (3'-5'): 3'dTdT GCCAC CAAUG AAACA GC 5'SEQ ID NO. 10
[0087] Si-GSTS1_003: ATATT ATTAT AAGCG AT
[0088] Sense strand (5'-3'): 5'AUAUU AUUAU AAGCG AU dTdT 3' SEQ ID NO. 11
[0089] Antisense strand (3'-5'): 3'dTdT UAUAA UAAUA UUCGC UA 5'SEQ ID NO.12
[0090] ...
Embodiment 3
[0110] This example especially provides preparation and effect research of chitosan nanoparticle biological preparation with double-stranded ribonucleic acid sequence of RIFA arginine kinase gene as an active ingredient.
[0111] (1) Design and synthesis of double-stranded ribonucleic acid drug for RIFA arginine kinase gene
[0112] GenBank: EU817514.1
[0113] Si-AK_001: TGGTA TCTAC GCGCC CGAC
[0114] Sense strand (5'-3'): 5'UGGUA UCUAC GCGCC CGAC dTdT 3'SEQ ID NO. 13
[0115] Antisense strand (3'-5'): 3'dTdT ACCAU AGAUG CGCGG GCUG 5'SEQ ID NO.14
[0116] Si-AK_002: CCGCT CACTG GCATG AGCA
[0117] Sense strand (5'-3'): 5' CCGCU CACUG GCAUG AGCA dTdT 3' SEQ ID NO. 15
[0118] Antisense strand (3'-5'): 3'dTdT GGCGA GUGAC CGUAC UCGU 5'SEQ ID NO. 16
[0119] Si-AK_003: GCATC GCTGA GCTCA TCAA
[0120] Sense strand (5'-3'): 5' GCAUC GCUGA GCUCA UCAA dTdT 3' SEQ ID NO. 17
[0121] Antisense strand (3'-5'): 3'dTdT CGUAG CGACU CGAGU AGUU 5'SEQ ID NO.18
[0122] The 3' end of ...
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