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Chitosan nano particle, biological pesticide preparation for controlling red fire ants and preparation method thereof

A technology of chitosan nanoparticles and biopesticides, which is applied in the fields of botany equipment and methods, biocides, animal repellants, etc., and can solve the physical and chemical indicators of unrealized standardization level, nanoparticle size distribution, and drug loading rate Instability, cumbersome process and other problems, to achieve the effect of industrialized large-scale operation, high pest control effect, and breakthrough operation scale

Active Publication Date: 2013-05-22
GUANGZHOU RIBOBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above reports have successfully prepared pesticide chitosan nanoparticle preparations, their research and development are still in the exploratory stage, and the physical and chemical indicators such as the size, particle size distribution, and drug loading rate of the prepared nanoparticles are unstable, and the standardization level has not been achieved. It is cumbersome and difficult to achieve large-scale production, so it has been limited to laboratory research so far, and has not been transformed into a product preparation circulation market
In addition, the active ingredients of the pesticide chitosan preparations successfully reported so far are mostly existing chemical pesticides with small molecular weight and relatively simple technical operation.

Method used

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  • Chitosan nano particle, biological pesticide preparation for controlling red fire ants and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling red fire ants and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling red fire ants and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation using the double-stranded ribonucleic acid sequence of the e-chain gene of the red fire ant ATP synthase as the active ingredient.

[0049] (1) Design and synthesis of double-stranded ribonucleic acid technical components for ATP synthase e-chain gene of RIFA GenBank: GU471223.1

[0050] Si-ATP_001: TCATC CGCGA GCAAG AT

[0051] Sense strand (5'-3'): 5'UCAUC CGCGA GCAAG AU dTdT 3' SEQ ID NO. 1

[0052] Antisense strand (3'-5'): 3'dTdT AGUAG GCGCU CGUUC UA 5'SEQ ID NO.2

[0053] Si-ATP_002: GTACGTGACG CCAAG CT

[0054] Sense strand (5'-3'): 5' GUACG UGACG CCAAG CU dTdT 3' SEQ ID NO.3

[0055] Antisense strand (3'-5'): 3'dTdT CAUGC ACUGC GGUUC GA 5'SEQ ID NO.4

[0056] Si-ATP_003: GTGCT GTTTC AACAT CA

[0057] Sense strand (5'-3'): 5' GUGCU GUUUC AACAU CA dTdT 3' SEQ ID NO. 5

[0058] Antisense strand (3'-5'): 3'dTdT CACGA CAAAG UUGUA GU 5'SEQ I...

Embodiment 2

[0079] This example particularly provides preparation and effect research of chitosan nanoparticle biological preparation with double-stranded ribonucleic acid sequence of red fire ant glutathione transferase gene as an active ingredient.

[0080] (1) Design and synthesis of double-stranded ribonucleic acid technical components for RIFA glutathione transferase gene GenBank: AY255670.1

[0081] Si-GSTS1_001: TAGAC TGCAA GAACA TG

[0082] Sense strand (5'-3'): 5'UAGAC UGCAA GAACA UG dTdT 3'SEQ ID NO.7

[0083] Antisense strand (3'-5'): 3'dTdT AUCUG ACGUU CUUGU AC 5'SEQ ID NO.8

[0084] Si-GSTS1_002: CGGTG GTTAC TTTGT CG

[0085] Sense strand (5'-3'): 5'CGGGUGGUUAC UUUGUCG dTdT 3'SEQ ID NO.9

[0086] Antisense strand (3'-5'): 3'dTdT GCCAC CAAUG AAACA GC 5'SEQ ID NO. 10

[0087] Si-GSTS1_003: ATATT ATTAT AAGCG AT

[0088] Sense strand (5'-3'): 5'AUAUU AUUAU AAGCG AU dTdT 3' SEQ ID NO. 11

[0089] Antisense strand (3'-5'): 3'dTdT UAUAA UAAUA UUCGC UA 5'SEQ ID NO.12

[0090] ...

Embodiment 3

[0110] This example especially provides preparation and effect research of chitosan nanoparticle biological preparation with double-stranded ribonucleic acid sequence of RIFA arginine kinase gene as an active ingredient.

[0111] (1) Design and synthesis of double-stranded ribonucleic acid drug for RIFA arginine kinase gene

[0112] GenBank: EU817514.1

[0113] Si-AK_001: TGGTA TCTAC GCGCC CGAC

[0114] Sense strand (5'-3'): 5'UGGUA UCUAC GCGCC CGAC dTdT 3'SEQ ID NO. 13

[0115] Antisense strand (3'-5'): 3'dTdT ACCAU AGAUG CGCGG GCUG 5'SEQ ID NO.14

[0116] Si-AK_002: CCGCT CACTG GCATG AGCA

[0117] Sense strand (5'-3'): 5' CCGCU CACUG GCAUG AGCA dTdT 3' SEQ ID NO. 15

[0118] Antisense strand (3'-5'): 3'dTdT GGCGA GUGAC CGUAC UCGU 5'SEQ ID NO. 16

[0119] Si-AK_003: GCATC GCTGA GCTCA TCAA

[0120] Sense strand (5'-3'): 5' GCAUC GCUGA GCUCA UCAA dTdT 3' SEQ ID NO. 17

[0121] Antisense strand (3'-5'): 3'dTdT CGUAG CGACU CGAGU AGUU 5'SEQ ID NO.18

[0122] The 3' end of ...

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Abstract

The invention discloses a chitosan nano particle which comprises the following raw materials in percentage by weight: 0.5-5% of chitosan, 1-10% of cosolvent, 0-5% of antifreezing agent, 0.1-1% of surfactant, 0.1-0.5% of emulsifier, 40-60% of buffer solution and the balance of water. The invention also provides a biological pesticide preparation for controlling red fire ants and a preparation method thereof. The biological pesticide preparation mainly comprises the following components in percentage by weight: (A) 0.05-15% of active component: a siRNA (small interfering ribonucleic acid) is designed and synthesized in accordance with a red fire ant high-efficiency killing target gene and is a 15-50nt double-chain RNA; (B) 19.95-55% of solvent; and (C) 30-80% of chitosan nano particle. The chitosan nano particle ensures that the stability and the red fire ant control effect of the biological pesticide preparation are better.

Description

technical field [0001] The invention belongs to the technical field of agricultural pesticides, and in particular relates to a biological pesticide preparation and a preparation method thereof, in particular to a chitosan nanoparticle, a double-stranded RNA-containing biological pesticide preparation for controlling RIFA and a preparation method thereof. technical background [0002] Red fire ants (Solenop sis invicta Buren), belonging to Hymenoptera, Formicidae, subfamily Mymicinae, fire ant genus Solenopsis, also known as alien red fire ants and invasive red fire ants, are omnivorous pests. , with ferocious habits, rapid reproduction and strong competitiveness, it has serious harm to human health, public safety, agricultural and forestry production and ecological environment in the invaded area, so it is listed as one of the 100 most dangerous invasive pests in the world. [0003] The RIFA was originally distributed in the Paraná River Basin of South America. The RIFA inva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N57/16A01P7/04
Inventor 张必良
Owner GUANGZHOU RIBOBIO
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