Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of low-molecular-weight heparin

A technology of low molecular weight heparin and molecular weight, which is applied in the field of improving enzymatic extraction of low molecular weight heparin with high thrombin activity, and can solve problems such as side effects

Active Publication Date: 2013-04-24
青岛亚博生物科技有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of mammalian-derived LMWHs has raised concerns about side effects due to the recognition of intergeneric cross-viral and prion infections among different species of mammals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of low-molecular-weight heparin
  • Preparation method of low-molecular-weight heparin
  • Preparation method of low-molecular-weight heparin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027]

[0028] The implementation of the present invention will be described in detail below in conjunction with the accompanying drawings and examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for preparing low-molecular-weight heparin by using non-mammal fish resources. Specific steps comprise: ungraded heparin component is obtained by rough extraction from fish tissues; and the ungraded heparin is degraded by using heparanase, such that low-molecular-weight heparin lyophilized pure product is obtained. The content is calculated with a carbazole colorimetric method; molecular weight and purity are analyzed with a high-performance liquid chromatography-mass spectrometer and a polyacrylamide gel electrophoresis method; and activity evaluation is carried out by determining anti-IIa titer with a sheep plasma method and by determining anti-Xa titer with a chromogenic substrate method. According to the invention, non-mammal fish resources are utilized, such that adverse influences caused by inter-generic cross virus and prion infection existing among different species of mammals are overcome. Also, an enzyme extraction method is improved, such that the high-thrombin-activity low-molecular-weight heparin is finally obtained.

Description

technical field [0001] The invention relates to a preparation method of low-molecular-weight heparin, in particular to a method for extracting high-thrombin-active low-molecular-weight heparin by using non-mammalian fish resources through an improved enzymatic method. [0002] Background technique [0003] Coagulation is a physiological pathway involved in maintaining normal hemostasis in mammals. In the case of blood vessel injury, the coagulation pathway is stimulated to form a blood clot to prevent blood loss. Immediately after the blood vessel injury occurs, platelets begin to aggregate at the injured site to form a physical blockage to Stop blood loss. In addition, injured blood vessels also reduce blood flow through the area through vasoconstriction, and fibrin begins to clump together to form an insoluble network, or clot, that covers the ruptured area. [0004] When the coagulation pathway is in an imbalance that favors excessive clotting, the result is a tendency ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/26
Inventor 曲辉张春革李萍张永勤
Owner 青岛亚博生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com