Reagent concentration responsive microfluidic cell culture chip and preparation method thereof

A microfluidic chip and cell culture technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as no substantive breakthroughs, achieve good application prospects, reduce Dosage, the effect of simplifying the cell implantation process

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, microfluidic chips are used to fabricate microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannels to drive sample microfluidics, and at the same time complete cell implantation and study the effect of different biological reagent concentrations on temperature. The technology of cell growth influence has not yet had a substantial breakthrough in related application fields

Method used

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  • Reagent concentration responsive microfluidic cell culture chip and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip suitable for cell culture that contains six inlets, one main channel for cell culture, and six outlets. Add the cell mixture from the upper solution inlet to control the liquid difference height between the inlet and the outlet, thereby controlling the l...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip suitable for cell culture that contains six inlets, one main channel for cell culture, and six outlets. Add the cell mixture from the upper solution inlet to control the liquid difference height between the inlet and the outlet, thereby controlling the l...

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Abstract

The invention relates to a reagent concentration responsive microfluidic cell culture chip and a preparation method thereof. A microstructure and a microchannel are arranged on the surface of the microfluidic chip; the surface of the microchannel is modified by using bovine serum albumin cells; the cells are implanted in the microchannel by utilizing the laminar flow phenomenon between liquid flow under the drive of gravity generated by liquid difference between a solution inlet and a solution outlet; and cell culture solutions of biological reagents with different concentrations are added into the solution inlet at last. The microfluidic chip can be used for making a research on the influence of reagent concentration change on cell culture so as to lay a foundation for cell biology research, and is mainly applied to the related fields of cell biology, genetics, drug screening and the like. The microfluidic chip is simple to operate, can be used for finishing cell implantation and culture, reducing the usage amount of reagents and samples, simplifying the cell implantation process and making a research on the influence of reagent concentration change on cell culture, and has the characteristics of being portable, economic, quick, efficient and accurate, thereby providing a brand-new cell culture and analysis technology for cell biology research.

Description

technical field [0001] The invention relates to a reagent concentration-responsive microfluidic cell culture chip and a preparation method thereof. The surface of the microfluidic chip has a microstructure and a microchannel, and the surface of the microchannel is modified with bovine serum albumin molecules. Driven by the gravity caused by the liquid difference between them, the laminar flow phenomenon between the liquid flows is used to realize the implantation of cells in the microchannel, and finally, cell culture solutions with different concentrations of biological reagents are added from the solution inlet to realize the change of reagent concentration. The impact on cell culture lays the foundation for the study of cell biology, and is mainly used in related fields such as cell biology, genetics, and drug screening. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 叶嘉明沙俊王晓东聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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