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Supercritical extraction pollen extractive as well as preparation and application thereof

A supercritical and extractive technology, which is applied in the direction of drug combinations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of unrevealed chemical ingredients, large loss of volatile substances, and destruction of active ingredients, etc., and achieve good reliability. Effects of regulation and selectivity, easy refining, and good flow properties

Active Publication Date: 2013-04-17
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The mechanism of action written in its specification is to inhibit 5α-reductase to convert testosterone into dihydrotestosterone, but the chemical components that work have not been revealed
In addition, many of the above-mentioned patent documents use organic solvents such as ethanol and acetone for extraction, which are not only flammable and explosive during the production process, but also cause harm to the environment, and high temperature is required to evaporate the solvent in the extraction solution, and terpenes, etc. are volatilized during the concentration process. The material loss is large, the active ingredient is destroyed, and the organic solvent extraction will inevitably cause toxic residues on the final product, which will affect its final application

Method used

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  • Supercritical extraction pollen extractive as well as preparation and application thereof
  • Supercritical extraction pollen extractive as well as preparation and application thereof
  • Supercritical extraction pollen extractive as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Poppy poppy pollen (collected from Wuwei, Gansu Province) → Weigh 3 g → Add a 30 ml extraction kettle (a little absorbent cotton is placed in the bottom of the extraction kettle in advance to prevent the outlet pipeline from being blocked), tighten the lid of the extraction kettle → supercritical CO 2 The extractor (SFE-prime, American ASI company) extracted for 2 h, the extraction temperature was 40 °C, and the extraction pressure was 300 bar→after the extraction, the supercritical extract was collected in a small glass bottle, and its 5α-reductase inhibitory activity was tested in vitro. Experiment, measured its IC 50 =138 μg / mL.

Embodiment 2

[0043] Schisandra pollen (collected from Linhe, Inner Mongolia) → Weigh 4 g → Add a volume of 30 ml to the extraction kettle (a little absorbent cotton is placed in the bottom of the extraction kettle in advance to prevent the outlet pipeline from being blocked), tighten the lid of the extraction kettle → supercritical CO 2 The extractor (SFE-prime, American ASI Company) extracted for 3 h, the extraction temperature was 53 °C, and the extraction pressure was 350 bar → after the extraction, the supercritical extract was collected in a small glass bottle, and its 5α-reductase inhibitory activity was tested in vitro. Experiment, measured its IC 50 =92 μg / mL,

Embodiment 3

[0045] Corn pollen (collected from Changzhi, Shanxi) → Weigh 5g → Add a 30 ml extraction kettle (a little absorbent cotton is placed in the bottom of the extraction kettle in advance to prevent the outlet pipeline from being blocked), tighten the lid of the extraction kettle → supercritical CO 2 The extractor (SFE-prime, American ASI Company) extracted for 1.5 h, the extraction temperature was 45 °C, and the extraction pressure was 250 bar → after the extraction, the supercritical extract was collected in a small glass bottle, and its 5α-reductase inhibitory activity was tested in vitro. Experiment, measured its IC 50 =138 μg / mL,

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Abstract

The invention relates to a supercritical extraction pollen extractive as well as preparation and application thereof, wherein the extractive has high safety and 5 alpha-reductase inhibitory activity. The supercritical extraction pollen extractive is prepared by the following method: a pollen raw material is weighed, and is added in an extraction kettle; a lid of the extraction kettle is tightened; then the pollen raw material is extracted for 1-3 hours by a supercritical CO2 extraction instrument at the temperature of 30-60 DEG C under the pressure of 200-500 bar; and the supercritical extraction pollen extractive is collected after the extraction is finished; the pollen raw material is selected from papaver rhoeas, schisandra chinensis or corn. The method is adopted to obtain three supercritical extraction pollen extractives with better 5 alpha-reductase inhibitory activity; the source of raw materials is safe; the supercritical extraction process adopted by the method dose not relate to poisonous and harmful organic solvent; and prepared products have lower poison and side effects, have high safety, and bring convenience to develop subsequent health care foods.

Description

(1) Technical field [0001] The invention relates to a highly safe pollen supercritical extraction extract with 5α-reductase inhibitory activity and its preparation and application. (2) Background technology [0002] Benign prostatic hyperplasia (BPH) refers to the benign adenomatous hyperplasia of cells near the urethra. The proliferated prostate squeezes the urethra, causing a series of symptoms of urination disorders, such as frequent urination, urgency at night, weak urine flow, and urinary incontinence. Equal voiding disorder seriously affects the quality of life of patients, and has become one of the main diseases affecting the health and quality of life of the elderly worldwide. In the United States, 70% of men over the age of 50 suffer from BPH. Prostate cancer ranks first in the incidence of male cancer in the United States and second in its death rate. With the aging of the social population, the incidence of BPH in my country is also increasing year by year. At pr...

Claims

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Application Information

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IPC IPC(8): A61K36/57A61K36/66A61K36/899A61P13/08A61P35/00
Inventor 杨开孙培龙蔡敏高粱何荣军
Owner ZHEJIANG UNIV OF TECH
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