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Kit for detecting mitochondria DNA A4263G related to hypertension and application

A DNAA4263G, mitochondrial technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as trouble, and achieve the effects of reducing pain, low cost, and simple detection process

Active Publication Date: 2013-04-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This blood collection method has brought some pain and injury to many subjects, especially infants and young children, and there are certain risks and troubles when transferring samples across regions

Method used

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  • Kit for detecting mitochondria DNA A4263G related to hypertension and application
  • Kit for detecting mitochondria DNA A4263G related to hypertension and application
  • Kit for detecting mitochondria DNA A4263G related to hypertension and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Detection of essential hypertension family members carrying mitochondrial gene A4263G mutation, see figure 1 .

[0039] 1. Test samples

[0040] A pedigree with essential hypertension carrying A4263G mutation was selected. See his pedigree figure 2 . This family showed typical maternal inheritance, and the only clinical symptom was elevated blood pressure in all patients, but the degree of elevated blood pressure of each affected member in the family varied. The total number of members in this family is 104, of which 27 are members of the maternal line, and 8 suffer from hypertension.

[0041] 2. Extraction of Genomic DNA

[0042] Samples from 5 subjects were obtained respectively (including a drop of venous blood filter paper from capillaries of III-14, II-4 and III-13; hair follicles on III-2 and III-18), and the blood filter paper Use clean scissors to cut into pieces of paper about 1cm2 in size and put them into a 1.5ml EP tube (Eppendorf tube), a...

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Abstract

The invention provides a kit for detecting mitochondria DNA A4263G related to hypertension. The kit consists of a primer sequence, a reagent for extracting a sample genome DNA, a polymer chain reaction (PCR) amplified reaction reagent, a positive sample contrast, a negative sample contrast and an operating manual. According to the kit, the genome DNA is extracted from a small amount of samples (such as a venous blood drop from blood capillary, hair with hair follicle, mouth mucosa blade scraping or spit) by a proteinase K digestion lysis method; segmental nested PCR amplification is performed by the design of primers; and the mitochondria mutation sites related to the hypertension are detected by specific restriction enzyme styI enzyme cutting identification. The kit implements non-invasive detection, is more fast, economic, simple and convenient than the separate detection for the mitochondria DNA A4263G mutation, has low requirement on the equipment and environment, and is favorable for popularization and application and suitable for the extensive screening and preventive inspection for the related population of hypertension.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a kit for detecting the mitochondrial DNA A4263G mutation associated with essential hypertension, and also relates to a detection method for the mitochondrial gene mutation associated with essential hypertension, in particular to the detection of mitochondria tRNA Ile The method for the A4263G mutation, and the application of the above method or the above kit in detecting the mitochondrial DNA A4263G mutation related to essential hypertension. Background technique [0002] Cardiovascular disease is the leading cause of death worldwide, including a series of diseases involving the circulatory system (mainly the heart and blood vessels) such as coronary heart disease, hypertension, heart attack, stroke, cardiomyopathy and heart failure. According to the statistics of the World Health Organization (WHO), about 17.3 million people died of cardiovascular diseases in 2008, accounti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 管敏鑫蒋萍萍冀延春肖云刘浩
Owner ZHEJIANG UNIV
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