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Process of extracting abamectin components B1 and B2 step by step by utilizing crystallization method

A technology of abamectin and crystallization method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve the effects of reducing waste generation, less equipment modification, and improving enterprise economic benefits

Active Publication Date: 2013-04-10
内蒙新威远生物化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the fermentation process of this product, the content of B1 (B1a and a small amount of B1b) component and B2 (B2a and a small amount of B2b) component is basically the same, but the existing extraction process only extracts and purifies the B1 component, but does not extract and purify the B2 component. The components are separated and purified, and the research shows that the B2 component has good effects as a pesticide and a veterinary drug

Method used

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  • Process of extracting abamectin components B1 and B2 step by step by utilizing crystallization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The abamectin fermentation broth is filtered to collect mycelium, dried to a moisture content of 40%, and 1,000 kilograms of the above-mentioned mycelia are extracted for 6 hours with 8,000 liters of ethanol; Concentrate the leaching solution to abamectin content of 1.5%, add 3 kg of activated carbon, decolorize at 40-70 degrees Celsius for 30 minutes, then filter to remove the activated carbon; continue to evaporate the filtrate to remove solvent, add 300 liters of toluene, heat up to 70 degrees Dissolve, then cool down to 10°C at 3-5°C / h to crystallize to obtain a mixture of Abamectin B1 component and B2 component ①201 kg; evaporate all the solvent from the crystallization mother liquor, add 100 liters of methanol to melt at 65°C and cool down Crystallize at 10°C to obtain 10.4 kg of Abamectin B1 component (3), wherein the mass ratio of B1a component to B1b component is 22.

[0022] The mixture of B1 component and B2 component obtained from the first crystallization ①...

Embodiment 2

[0026] Collect the mycelium by filtering the abamectin fermentation liquid, dry it to a moisture content of 10%, take 1000 kg of the above-mentioned mycelium and use 1m 3 Ethanol leaching for 6 hours; after leaching, filter the mycelium to obtain the leaching solution; concentrate the leaching solution to abamectin content of 0.5%, add 5 kg of activated carbon, decolorize at 40-70 degrees Celsius for 30 minutes, and then filter Remove the activated carbon; continue to evaporate the filtrate to remove the solvent, add 600 liters of toluene, heat up to 70°C to fully dissolve, then cool down to 10°C to crystallize, and obtain a mixture of abamectin B1 and B2 components ① 211 kg; the crystallization mother liquor is evaporated and removed Add 110 liters of methanol to dissolve all the solvents at 65° C. and then cool down to 10° C. to crystallize to obtain 10.1 grams of Abamectin B1 component ③ 10.1 grams, wherein the mass ratio of B1a component to B1b component is 21.

[0027] Th...

Embodiment 3

[0031] The abamectin fermentation broth is filtered to collect mycelium, dried to a moisture content of 70%, and 1000 kg of the above-mentioned mycelium is used in a 1.5m 3 Ethanol leaching for 6 hours; after leaching, filter the mycelium to obtain the leaching solution; concentrate the leaching solution to abamectin content of 2.5%, add 8 kg of activated carbon, decolorize at 40-70 degrees Celsius for 30 minutes, and then filter Remove the activated carbon; continue to evaporate the filtrate to remove the solvent, add 900 liters of toluene, heat up to 70°C to fully dissolve, then cool down to 10°C to crystallize, and obtain a mixture of abamectin B1 and B2 components ①198 kg; the crystallized mother liquor is evaporated and removed All the solvents were dissolved in 120 liters of methanol at 65° C. and then cooled to 10° C. to crystallize to obtain 10.9 grams of Abamectin B1 component ③ 10.9 grams, wherein the mass ratio of B1a component to B1b component was 22.

[0032] The ...

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Abstract

The invention relates to a process of extracting abamectin components B1 and B2 step by step by utilizing a crystallization method, comprising the steps of leaching abamectin mycelia by using ethanol (or methanol), subjecting the leachate to preliminary concentration, adding active carbon to decolorize the leachate, converting the solvent into toluene after the decolorization is completed, carrying out cooling crystallization to obtain a mixture of the abamectin components B1 and B2, converting the solvent of the crystallization mother liquor into methanol, carrying out cooling crystallization on the mixture of the abamectin components B1 and B2, obtained in the first crystallization, to obtain crystals i.e. the abamectin component B1, converting the solvent of the mother liquor into toluene and obtaining the components B2 through crystallization. According to the invention, on the premise of not increasing the fermentation cost, the components B1 and B2 are extracted from the mycelia step by step, so that the economic benefit of enterprises is increased, and the resource waste and the waste production are reduced at the same time.

Description

technical field [0001] The invention relates to a process for extracting abamectin B1 component and B2 component step by step by using a crystallization method. Background technique [0002] Abamectin is a fermentation metabolite of Streptomyces griseus avermanii, which belongs to antibiotic drugs. B2a, B2b and other 8 components (see formula 1 for the structure of each component), among the eight components, the biological activity of B component (B1a, B1b, B2a, B2b) is better than that of A component (A1a, A1b , A2a, A2b). During the fermentation process of this product, the content of B1 (B1a and a small amount of B1b) component and B2 (B2a and a small amount of B2b) component is basically the same, but the existing extraction process only extracts and purifies the B1 component, but does not extract and purify the B2 component. The components are separated and purified, and studies have shown that the B2 component has good effects as both a pesticide and a veterinary dr...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/06
Inventor 张庆高鹤永杨军强王少伟吕烨次素英
Owner 内蒙新威远生物化工有限公司
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