Long-distance transportation method of sepia esculenta adults
A technology for long-distance transportation and golden squid, applied in fish farming, application, climate change adaptation, etc., can solve problems such as no golden squid, achieve long survival time, ensure transportation water quality, and avoid physical damage
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Embodiment 1
[0026] (1) According to the stage of anesthesia and resuscitation behavior of golden squid, then determine the appropriate concentration of golden squid anesthesia operation and anesthesia transportation
[0027] Experimental design 2, 5, 8, 15, 20, 30, 40, 60 and 80mg / L9 concentration gradients, each concentration test 5 adult golden squid, determine the final anesthesia degree of golden squid within 24 hours, and observe the final anesthesia Then transfer to clean seawater for resuscitation, observe the behavioral characteristics during the resuscitation process, and then stage the degree of anesthesia and the resuscitation process.
[0028] Test results: 1) The degree of anesthesia was divided into six periods according to the behavioral characteristics in the final anesthesia state, see Table 1.
[0029] Table 1 Stages of degree of anesthesia and behavioral characteristics
[0030] stage of anesthesia
[0031] 2) According to the recovery characteristics, the re...
Embodiment 2
[0036] In June 2012, we transported 250 adult golden cuttlefish from Qingdao Jinshatan Aquaculture Development Co., Ltd. to Tianjin Xinyongfeng Aquaculture Co., Ltd. Cool the transport water with ice cubes from 24°C to 18°C, add 7mg / L of MS-222, and then fill it with pure oxygen to saturation with nano-air stones; add ice cubes or ice bottles to the golden squid breeding ponds that have stopped eating for 1 day to cool down, and after 2 The temperature difference should not exceed 3 °C each time, and the temperature was lowered to 18 °C; then MS-222 with a final concentration of 30 mg / L was added, and the golden squid entered the fourth stage of anesthesia after 5 minutes, that is, the wrists were spread out, and the sideways The fins stop swinging and the sense of balance is lost. Immediately fish the golden squid gently into the washbasin, weigh it, slowly fish it out by hand and put it into a black plastic bag with 10L of transport water, and put 1 adult golden squid in each...
Embodiment 3
[0038]In July 2012, we transported 150 adult golden squid from Huangdao golden squid experimental base of Yellow Sea Fisheries Research Institute to Xinhaixin Nursery Farm in Xiapu, Fujian. Cool the transport water to 16°C with ice cubes, add 8mg / L MS-222, and then fill it with pure oxygen to saturation with nano air stones; add ice cubes or ice bottles to the golden squid breeding ponds that have stopped eating for 1 day to cool down, and after 3 times gradually Step-by-step cooling treatment, the temperature difference does not exceed 5°C each time, and the temperature is lowered to 16°C; then MS-222 with a final concentration of 60 mg / L is added, and the golden squid enters the fourth stage of anesthesia after 3 minutes, that is, the wrists are spread out, and the side fins stop. Shake and lose the sense of balance. Immediately fish the golden squid gently into the washbasin, weigh it, slowly fish it out by hand and put it into a black plastic bag with 10L of transport water...
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