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Method for screening lactobacillus plantarum ST-III salt-tolerance gene

The technology of Lactobacillus plantarum and salt tolerance gene is applied in the field of screening Lactobacillus plantarum ST-III salt tolerance gene, which can solve the problem that bacteria are not completely involved, and achieve the effect of strong salt tolerance and improved salt tolerance.

Active Publication Date: 2013-04-03
JIANGNAN UNIV
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Problems solved by technology

However, these patents or patent applications do not fully relate to the screening of bacteria, especially the salt tolerance genes of probiotic lactic acid bacteria

Method used

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  • Method for screening lactobacillus plantarum ST-III salt-tolerance gene
  • Method for screening lactobacillus plantarum ST-III salt-tolerance gene
  • Method for screening lactobacillus plantarum ST-III salt-tolerance gene

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Embodiment Construction

[0031] In view of the current lack of molecular information on the salt-tolerance mechanism of Lactobacillus plantarum, the salt-tolerant gene of Lactobacillus plantarum ST-Ⅲ was discovered for the first time using RNA-seq technology, and the culture of Lactobacillus plantarum ST-Ⅲ without NaCl and 6% NaCl was obtained. Transcripts of gene expression during culture in medium. The present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0032] The screening method of Lactobacillus plantarum ST-Ⅲ salt tolerance gene of the present invention preferably comprises the following steps in sequence:

[0033] 1. Handling of experimental materials

[0034] (1) Streak Lactobacillus plantarum ST-Ⅲ on a chemically defined medium (CDM) solid plate, culture it upside down at 37°C until a single colony grows, pick a single colony and inoculate it into liquid CDM medium, and culture it statically at 37°C 12h;

[0035] (2) The ab...

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Abstract

The invention relates to a method for screening a lactobacillus plantarum ST-III salt-tolerance gene. The method adopts lactobacillus plantarum ST-III as a material, a ribonucleic acid (RNA)-sequence (seq) technology is applied to carry out transcriptome sequencing analysis, the lactobacillus plantarum ST-III salt-tolerance gene is screened quickly and comprehensively, and the method has an important guiding significance for lactobacillus plantarum ST-III salt-tolerance mechanisms, probiotic performance and product development.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a method for screening salt tolerance genes of Lactobacillus plantarum ST-III. Background technique [0002] Lactic acid bacteria (Lactic Acid Bacteria, LAB) are one of the earliest bacteria used by humans. These bacteria have long been used to produce animal (milk, meat, fish, etc.) and plant (vegetables, wine, etc.) fermented food. Lactic acid bacteria will encounter high salt concentration during fermentation, such as fermented cheese, sausage, fish, soy sauce, vegetables and food preservation. The hyperosmotic environment caused by high salt concentration will lead to structural and physiological damage of cells. Lactobacillus plantarum ST-Ⅲ (Lactobacillus plantarum ST-Ⅲ) is a lactic acid bacterium isolated from pickles. It has strong adhesion, has the probiotic properties of removing cholesterol, and has strong penetration resistance. It can e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/02
Inventor 陈卫赵山山张秋香刘小鸣陈臣张灏
Owner JIANGNAN UNIV
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