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Plant gene capable of identifying xanthomonas oryzae protein activator and application of plant gene

A single-cell bacteria and elicitor technology, applied in the gene field of biotechnology, can solve problems such as identification differences and different amino acid sequences, and achieve the effect of overcoming the loss of resistance and wide application prospects

Inactive Publication Date: 2013-04-03
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Although these Harpin proteins can be secreted from the type III system, their amino acid sequences are different, revealing that there are differences in the recognition of them by non-host indicator plants

Method used

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  • Plant gene capable of identifying xanthomonas oryzae protein activator and application of plant gene
  • Plant gene capable of identifying xanthomonas oryzae protein activator and application of plant gene
  • Plant gene capable of identifying xanthomonas oryzae protein activator and application of plant gene

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with specific embodiments. Experimental methods without specific conditions in the examples usually follow conventional conditions, such as the conditions described in Sambrook et al. Molecular Cloning: Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989), or as recommended by the manufacturer condition.

[0019] 1. Cloning of HIP1 gene

[0020] In this example, the full length of the HIP1 gene in rice and tobacco was cloned, and the interaction with Hpa1 was verified by the yeast two-hybrid technique. First, with Xanthomonas oryzae vulgaris Hpa1 as bait, the interaction factors were screened from the rice cDNA library inoculated with Xanthomonas oryzae, and 4 clones were obtained. After sequencing, it was determined that these 4 fragments were all derived from the same gene HIP1. In the GenBank database, the HIP1 gene corresponds to Osh69, and the product is β-galactosidase. Using ric...

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Abstract

The invention provides a plant gene capable of identifying a xanthomonas oryzae protein activator. The plant gene comprises a rice HIP1 gene and a tobacco HIP1 gene, wherein the sequence of the rice HIP1 gene is shown as SEQ ID NO. 1 and the sequence of the tobacco HIP1 gene is shown as SEQ ID NO. 3; the rice HIP1 gene codes 754 amino acids of which the sequence is shown as SEQ ID NO. 2; and the tobacco HIP1 gene codes 510 amino acids of which the sequence is shown as SEQ ID NO. 4. After the overexpression of the rice HIP1 gene or the tobacco HIP1 gene and the protein of the rice HIP1 gene or the tobacco HIP1 gene in a plant, the disease resistance of the plant on viral disease and bacteriosis is enhanced, the rice HIP1 gene or the tobacco HIP1 gene can be used as resources for breeding disease resistant genes, the loss of plant resistance caused by the evolution of pathogenic bacteria is avoided and a new variety of durable-resistance transgenic crop is obtained.

Description

Technical field [0001] The present invention relates to a gene in the field of biotechnology, in particular to a plant gene capable of recognizing the protein elicitor of Xanthomonas oryzae. The gene exists in a variety of plants, and the encoded protein can be compatible with Xanthomonas oryzae. The Harpin protein Hpa1 interacts, and overexpression in plants enhances plant disease resistance. Background technique [0002] Phytopathogenic bacteria can infect host plants and cause symptoms near the infection point, which overcomes the immune system of the host plant and successfully obtains the required nutrients from the host plant. In the process of identifying pathogens and host plants, multiple genes and signal transduction mechanisms of both parties participate together. The type III secretion system of plant pathogenic bacteria is a key pathogenic factor that pathogens can infect and establish parasitic relationships. It is a gene cluster formed by nearly 30 genes, which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/56C12N9/38C12N15/84A01H5/00
Inventor 邹华松邹丽芳陈功友崔一平
Owner SHANGHAI JIAO TONG UNIV
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