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Cartilage tissue engineering fiber scaffold material and preparation method thereof

A technology of engineering fibers and scaffolding materials, applied in medical science, prosthesis, etc., can solve problems such as unsatisfactory performance and high cost, achieve good filamentation, overcome difficult molding, and reduce costs

Inactive Publication Date: 2013-04-03
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a cartilage tissue engineering fiber scaffold material made of konjac glucomannan, sodium hyaluronate and nano-hydroxyapatite and its preparation method, which solves the problem of existing cartilage tissue engineering scaffold materials. Unsatisfactory performance, high cost and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: The preparation method of this cartilage tissue engineering fiber scaffold material, specific content is as follows:

[0027] (1) Add 3g of sodium hyaluronate into the lactic acid solution and stir to fully dissolve it to obtain a sodium hyaluronate solution, wherein the lactic acid solution is prepared by mixing 1ml of lactic acid and 400ml of distilled water, and the mixing ratio of lactic acid and sodium hyaluronate is 1ml lactic acid mixed with 3g sodium hyaluronate;

[0028] (2) Add 0.03g of nano-hydroxyapatite to the sodium hyaluronate solution, mix well, add 3g of konjac glucomannan, and stir thoroughly to obtain a mixed glue solution, in which konjac glucomannan, sodium hyaluronate and The mass ratio of nano-hydroxyapatite is 1:1:0.01;

[0029] (3) Microwave heat the mixed glue solution for 5 minutes (microwave power is 900w), stir and cool to room temperature, filter through 200-mesh stainless steel mesh, and degas the filtrate under negative pre...

Embodiment 2

[0031] Embodiment 2: the preparation method of this cartilage tissue engineering fibrous scaffold material, specific content is as follows:

[0032] (1) Add 0.09g of sodium hyaluronate into the lactic acid solution and stir to fully dissolve it to obtain a sodium hyaluronate solution. The lactic acid solution is prepared by mixing 1.5ml of lactic acid and 400ml of distilled water. The mixture of lactic acid and sodium hyaluronate The ratio is 1ml lactic acid mixed with 0.06g sodium hyaluronate;

[0033] (2) Add 4.5g of nano-hydroxyapatite to the sodium hyaluronate solution, mix well, add 9g of konjac glucomannan, and stir thoroughly to obtain a mixed glue, in which konjac glucomannan, sodium hyaluronate and nano The mass ratio of hydroxyapatite is 1:0.01:0.5;

[0034] (3) Microwave heat the mixed glue solution for 15 minutes (microwave power is 500w), stir and cool to room temperature, filter through a 200-mesh stainless steel mesh, and degas the filtrate under a negative pre...

Embodiment 3

[0036] Embodiment 3: The preparation method of this cartilage tissue engineering fiber scaffold material, the specific content is as follows:

[0037] (1) Add 3g of sodium hyaluronate into the lactic acid solution and stir to fully dissolve it to obtain a sodium hyaluronate solution. The lactic acid solution is prepared by mixing 2ml of lactic acid and 400ml of distilled water. The mixing ratio of lactic acid and sodium hyaluronate is 1ml lactic acid mixed with 1.5g sodium hyaluronate;

[0038] (2) Add 0.6g of nano-hydroxyapatite to the sodium hyaluronate solution, mix well, add 6g of konjac glucomannan, and stir thoroughly to obtain a mixed glue, in which konjac glucomannan, sodium hyaluronate and nano The mass ratio of hydroxyapatite is 1:0.5:0.1;

[0039] (3) Microwave heat the mixed glue solution for 10 minutes (microwave power is 600w), stir and cool to room temperature, filter through a 200-mesh stainless steel mesh, and degas the filtrate under a negative pressure of 4...

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Abstract

The invention discloses a cartilage tissue engineering fiber scaffold material and a preparation method thereof. The method comprises the following steps: preparing mixed sol by mixing high-biocompatibility degradable biological materials such as konjac glucomannan, sodium hyaluronate and nano-hydroxyapatite which serve as basic materials; preparing modified sol by a lactic acid solution modifying and microwave process; performing wet spinning; and preparing a degradable fiber scaffold for regeneration and repair of a konjac glucomannan / sodium hyaluronate / nano-hydroxyapatite composite cartilage tissue by a programmable two-dimensional mobile platform. The scaffold has the advantages of simple preparation process, adjustable degradation rate, low cost, excellent tissue compatibility and the like. A preliminary cell test and an animal test indicate that the scaffold can be commonly used in vivo (such as cartilage defect lesion parts), and is a multifunctional composite material which can promote repair of cartilage defect, is antibacterial and can adjust the in-vivo degradation rate.

Description

technical field [0001] The invention relates to a cartilage tissue engineering fiber support material and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Once the articular cartilage is damaged, it is difficult to repair, because the fiber content in the articular cartilage is small, and the main chemical component of the matrix is ​​proteoglycan, which is different from vascularized tissues, because there is no blood supply, and the chondrocytes are buried in thickened tissue. In the thick extracellular matrix, it cannot move to the damaged site to participate in the repair, and it also lacks the ideal environment necessary for cell differentiation and cell migration, which makes it difficult for even a small cartilage defect to repair naturally. Therefore, until now, the repair of articular cartilage defects has been a clinical problem. At present, the traditional clinical treatment methods mainly include microfr...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/20
Inventor 陈庆华李音冷崇燕颜廷亭
Owner KUNMING UNIV OF SCI & TECH
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