Method for extracting oat starch, protein powder and beta-glucan from oat bran
A technology of oat bran and oat protein, applied in the fields of protein powder, β-glucan, and oat starch extraction, can solve problems such as waste, increased cost, and increased difficulty in wastewater treatment, and achieve full utilization of high added value and reduce The effect of production costs
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Embodiment 1
[0023] The method for extracting oat starch, protein powder and beta-glucan from oat bran comprises the following steps:
[0024] 1. Select high-quality oat bran that meets the requirements, soak it in warm water at 40°C, the ratio of material to liquid (water) is 1:7, and use a colloid mill to refine it.
[0025] 2. Use edible soda ash (sodium carbonate) solution to adjust the slurry to PH=10, stir at room temperature for 150 minutes, and separate the solid and liquid components with a spiral sedimentation centrifuge. The solid component mainly contains starch and gluten impurities, and the liquid component mainly contains Contains protein and beta-glucan.
[0026] 3. The liquid component is adjusted to PH=4.5 with food-grade hydrochloric acid, acetic acid, and citric acid, and stirred at the same time, the protein is precipitated, and the solid and liquid components are separated by a spiral sedimentation centrifuge. The filtrate mainly contains β-glucan, and the solid The ...
Embodiment 2
[0031] Purification of oat starch: Stir the underflow starch milk separated by the main centrifuge in Step 6 of Example 1, heat it to a temperature of 50°C, add neutral protease, the amount of enzyme added is 48AU / kg, the pH value is 7.0, and the time is 60 min , the residual amount of protein in the oat starch can be reduced to about 0.6%, and it is sent to a 12-stage hydrocyclone for countercurrent washing, and the oat starch is separated, dried and pulverized to obtain refined oat starch, and the yield of refined starch is about 1% of coarse starch 95%.
Embodiment 3
[0033] Purification of oat protein: add 5 times the volume of water to the solid component (oat protein) separated in step 3 of Example 1, stir, add α-amylase, the amount of enzyme added is 48-60AU / kg, PH=5- 6. The temperature is 30-40°C and the time is 30 minutes, so that the starch is fully hydrolyzed; the hydrolyzed oat protein solution is adjusted to PH=4.5 with food-grade hydrochloric acid, and then centrifuged to obtain a slurry oat protein concentrate; the oat protein concentrate is obtained by spray drying Refined oat protein powder.
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