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Method manufacturing micro-chamber plate for analysis and micro-chamber plate with samples, micro-chamber plate for analysis and apparatus set manufacturing micro-chamber plate with samples

A manufacturing method and technology of a manufacturing device, which are applied in the field of micro-chamber plates, can solve the problems of surface tension and centrifugal force obstruction, residual small bubble shape, and inability to obtain buoyancy, so as to reduce manufacturing costs, improve accuracy, and shorten analysis requirements. effect of time

Inactive Publication Date: 2013-03-27
BIONEER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0031] However, in the eighth method, the sample for injection is formed to be directly applied to the porous membrane, so 1) when using a vacuum-based sample injection method, when vacuum is applied, in order to prevent the sample from boiling Centrifugal force is applied, at this time, the discharge of gas passing through the pores of the porous membrane will be hindered by the surface tension of the sample and centrifugal force, 2) The gas in the cavity is compressed due to centrifugal force, and the volume becomes smaller, so it cannot be injected during injection. The buoyancy of the degree of detachment through the membrane is obtained, so that it remains in the form of small air bubbles, and under the measurement conditions of the atmospheric pressure state, it expands again, thus hindering the measurement

Method used

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  • Method manufacturing micro-chamber plate for analysis and micro-chamber plate with samples, micro-chamber plate for analysis and apparatus set manufacturing micro-chamber plate with samples
  • Method manufacturing micro-chamber plate for analysis and micro-chamber plate with samples, micro-chamber plate for analysis and apparatus set manufacturing micro-chamber plate with samples
  • Method manufacturing micro-chamber plate for analysis and micro-chamber plate with samples, micro-chamber plate for analysis and apparatus set manufacturing micro-chamber plate with samples

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Experimental program
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Effect test

Embodiment 1

[0090] Embodiment 1 relates to the method for manufacturing a microcavity plate for analysis provided by the present invention.

[0091] refer to figure 1 , Embodiment 1 includes the manufacturing step S10 of the microcavity plate for sample injection, the step S20 of arranging the microcavity plate for sample injection, the step S30 of arranging the cover body, the manufacturing step S40 of the microcavity plate containing the sample, and the manufacturing step S50 of the microcavity plate for analysis.

[0092] 1. Manufacturing step S10 of microcavity plate for sample injection

[0093] refer to Figure 2 to Figure 4 , in the microchamber plate manufacturing step S10 for sample injection, the microchamber plate 100 for sample injection containing the specific component 140 for analyzing nucleic acid including primers or probes is manufactured. The microchamber plate 100 for sample injection includes a microchamber body 110 , a body sealing portion 120 , and a separation me...

Embodiment 2

[0140] The second embodiment relates to the manufacturing method of the sample-containing microcavity plate provided by the present invention.

[0141] Embodiment 2 includes the manufacturing step S10 of the microcavity plate for sample injection, the step S20 of arranging the microcavity plate for sample injection, the step S30 of arranging the cover body, and the manufacturing step S40 of the microcavity plate with samples as described in Embodiment 1.

[0142] The description of this follows the description of the first embodiment.

Embodiment 3

[0144] The third embodiment relates to the analysis microcavity plate provided by the present invention. Analytical microcavity plates (not shown) except for the microcavity plate 100A inside the sample (refer to Figure 17 ) and the separation membrane 130 part, others are the same as above, so refer to Figure 17 Be explained.

[0145] refer to Figure 17 as well as Figure 16 , the third embodiment has a source (origin) microcavity main body 110-1 that penetrates up and down to form a unit number of source (origin) cavity holes 110-1H.

[0146] refer to Figure 17 and Figure 7 , the surface of the source microcavity body 110-1 and the unit number of source cavity holes 110-1H (refer to Figure 6 ) forms a polymer coating layer 110-2 on the inner surface. According to the polymer coating layer 110-2, the source cavity hole 110-1H corresponding to the unit number is formed (refer to Figure 6 ) of the unit number of cavities 112 .

[0147] refer to Figure 17 and ...

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Abstract

PURPOSE: A micro chamber plate is provided to prevent measuring error of an optical measuring unit due to contamination and to reduce time for analysis. CONSTITUTION: A method for manufacturing a sample-containing micro chamber plate comprises: a step of placing a micro chamber plate for sample injection for placing the micro chamber plate into a micro chamber plate receptor(S20); a step of placing a micro chamber plate receptor cover to cover the upper opening of the micro chamber plate receptor(S30); a step of inputting the micro chamber plate receptor into a centrifuge and applying centrifugal force; and a step of injecting a sample solution to the micro chamber plate(S40).

Description

technical field [0001] The present invention relates to a microchamber plate, in particular to a chamber plate for analysis that allows multiple reaction solutions to react without cross-contamination, thereby enabling real-time measurement and analysis of fluorescence values. A primer or probe that selectively reacts with each nucleic acid in a biological sample solution of nucleic acid. [0002] Furthermore, the present invention relates to a method of manufacturing the analysis chamber plate. [0003] Furthermore, the present invention relates to a method of manufacturing a sample-containing micro-cavity plate used in the production of the analysis chamber plate. [0004] Also, the present invention relates to a kit of devices for manufacturing said sample-containing microcavity plate. Background technique [0005] The so-called microcavity refers to a container for micro-reactions below several microliters. The microcavity can be formed by silicon wafers, glass, metal,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12M1/34G01N33/48G01N1/16
CPCB01L2300/0681B01L2300/048B01L2400/049B01L2200/16B01L2400/0409B01L3/50851B01L3/00B01L2300/044B01L2200/12B01L3/50255C12M23/12G01N21/6454B81B1/00B01L2300/0829Y10T29/51Y10T137/0402G01N1/16B01L3/502
Inventor 朴翰浯宋邱永裴贞娥
Owner BIONEER
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