Method for determining significant correlation between physicochemical property of rhizosphere soil of S-amino-acid-rich transgenic soybeans and diversity of microbial communities
A technology of microbial community and rhizosphere soil, applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc.
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[0010] Table 1 Soybean varieties used in the test
[0011]
[0012] As shown in Table 1, different lines of S-amino acid-rich transgenic soybean were used in the experiment, and their non-transgenic recipient varieties were used as controls. Three replicates were set up for each sample, and they were planted in the Mid-Autumn Festival in the field net room in 2009. Taking soybean flowering rhizosphere soil as the test material, the pH value, carbon-nitrogen ratio and sulfur content of each rhizosphere soil sample were measured respectively, and the obtained microbial community PLFA, PCR-DGGE and Biolog (GN2, GP2, FF and ECO plate ) data are applied to the measure of diversity index. The index formula for measuring soil microbial community diversity is shown in Table 2.
[0013] Table 2 Formula of soil microbial community diversity index
[0014]
[0015] All soil samples were subjected to 3 repeated independent experimental treatments. All data were processed by Exce...
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