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Bovine lactoferricin and preparation method thereof

A technology of bovine lactoferrin peptide and fusion protein, which is applied in the field of bovine lactoferrin peptide and its preparation, and can solve problems such as reducing the amount of use

Inactive Publication Date: 2013-03-27
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although my country has not yet legislated to prevent the use of antibiotics as feed additives, it is also gradually reducing the amount of use

Method used

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  • Bovine lactoferricin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] Embodiment genetic engineering produces bovine lactoferrin peptide

[0046] 1. Construction of recombinant expression vector

[0047] 1.1. Synthesis of Bovine Lactoferricin I Gene

[0048] The first 1-121 amino acid sequences of artificially synthesized bovine lactoferrin: (the underlined part is bovine lactoferrin peptide (total 363bp, 121 amino acids)

[0049] GCT CCA AGA AAG AAC GTT AGA TGG TGT ACT ATT TCT CAA CCA GAA

[0050] TGG TTT AAG TGT AGA AGA TGG CAA TGG AGA ATG AAG AAG TTG GGT

[0051] GCT CCA TCT ATT ACT TGT GTT AGA AGA GCT TTC GCT TTG GAA TGT

[0052] ATT AGA GCT ATT GCT GAG AAG AAG GCT GAT GCT GTT ACT TTG GAT

[0053] GGT GGT ATG GTT TT℃GAA GCT GGT AGA GAT CCA TAC AAG TTG AGA

[0054] CCA GTT GCT GCT GAA ATT TAC GGT ACT AAG GAA TCT CCA CAA ACT

[0055] CAT TAC TAC GCT GTT GCT GTT GTT AAG AAG GGT TCT AAC TTT CAA

[0056]TTG GAT CAA TTG CAA GGT AGA AAG TCT TGT CAT ACT GGT TTG GGT

[0057] AGA TT

[0058] Saved in pUC19 / TOP10.

[0059] 1.2 Clo...

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Abstract

The invention relates to bovine lactoferricin and a preparation method thereof. Bovine lactoferricin (Lfcin B) is 25 amino acid residues hydrolyzed from a bovine lactoferrin (BLF) N-terminal (17-41) by pepsin, and has a molecular weight of approximately 3.1kd. The preparation method comprises the steps of: (2) recombinant expression vector construction; (2) fusion protein expression induction; and (3) protein separation and purification. Bovine lactoferricin has the advantages that: Lfcin B has the highest activity among all the Lfcins, and an antibacterial effect 400 times that of bovine lactoferrin, such that Lfcin B can play an important role in gastrointestinal tracts.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bovine lactoferrin peptide and a preparation method thereof. Background technique [0002] Antibiotics have a long history of being used as antimicrobial and growth-promoting additives in animal feed, but with increasing awareness, the widespread use of antibiotics in animal feed has exposed more and more negative effects, the most serious of which are First, it can cause drug residues in livestock and poultry products, which directly affects the health of consumers; second, it seriously affects the micro-ecological balance of the animal digestive tract, resulting in the occurrence of drug resistance of some pathogenic bacteria, making it possible to treat the diseases caused by these pathogenic bacteria Prevention and treatment of infection becomes very difficult. At present, the problem of bacterial drug resistance is widespread, and the number of drug-resistant stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/79C07K1/22C12N15/12C12N15/63C12N1/21C12R1/19
Inventor 夏新界李圣翔
Owner GUANGZHOU GLAM BIOTECH
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