Fradimycin B as well as preparation method and application thereof

A technology of Vermycin and Streptomyces freundii, which is applied in the field of medicine, can solve the problems that the clinical effect does not meet the expected effect, and the drug resistance of cancer cells affects the curative effect, etc., and achieves good application prospects and significant antibacterial activity.

Inactive Publication Date: 2013-03-27
ZHEJIANG UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although molecular targeted drugs have opened up a new way for the treatment of cancer, their clinical effect has not reached the expected effect, and the increasing resistance of cancer cells to anticancer drugs has also seriously affected the efficacy of existing drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fradimycin B as well as preparation method and application thereof
  • Fradimycin B as well as preparation method and application thereof
  • Fradimycin B as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Vermycin B producing bacteria Streptomyces fradiae Culture isolation of PTZ0025

[0041] Take 5 g of marine sediment and place it in a 45 ml sterilized centrifuge tube and let it dry naturally in the air for 10 days. Dried samples were diluted to 0.01g with culture medium (5.0g glucose, 1.5g glycerol, 1.5g malt extract, 2.5g yeast extract, 0.5g casamino acids and 0.1g calcium carbonate per 100ml) / ml. Take 200 μl of the sample dilution and evenly disperse it on a bacterial agar (Bacto-agar) petri dish and incubate it at room temperature (20-28°C) for 10 days, then carefully select different colonies with sterilized inoculation needles and transfer them to another Bacterial agar plates were cultured at room temperature for 7 days. A single PTZ0025 colony that grew well was transferred to a Gossler synthetic agar slant medium and stored in a 4°C refrigerator for later use.

[0042] 2. Vermycin B producing bacteria Streptomyces fradiae Identification of strai...

Embodiment 2

[0080] Example 2 Bioactivity assay of Vermycin B

[0081] 1. Antibacterial activity of Vermycin B

[0082] The antibacterial effect of Vermycin B on Staphylococcus aureus in MH medium (Mueller-Hinton Broth) was determined. Vermycin B was made into 2 mg / ml dimethyl sulfoxide (DMSO) stock solution, and a certain amount of stock solution was diluted twice with DMSO to prepare a series of test sample solutions. In a 96-well micro-petri dish, mix 10 ml of each sample solution to be tested with 90 ml of MH medium containing logarithmic growth cells (bacterial cell density OD600 mm = 0.001) and mix under 37°C After hatching and cultivating for 18 hours, observe the test sample liquid of each concentration to Staphylococcus aureus ( Staphyloccocus aureus ) growth inhibition, to determine the minimum inhibitory concentration (MIC) of Vermycin B. The experimental results showed that the minimum inhibitory concentration (MIC) of Vermycin B against Staphylococcus aureus was 2.0 mg / ml...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a bioactive substance namely fradimycin B. The active monomeric compound namely the fradimycin B is prepared by the following steps of: separating streptomyces fradiae PTZ0025 which can generate the fradimycin B from ocean sediments, culturing the streptomyces fradiae PTZ0025, preparing a fermentation bacterial solution, centrifuging the fermentation bacterial solution at a high-speed, extracting, separating and purifying the fermentation bacterial solution, and performing structural identification. The fradimycin B has an obvious antimicrobial activity on staphylococcus aureus, can be applied to the preparation of medicaments for treating infectious diseases which are caused by the staphylococcus aureus, can obviously inhibit the growth of intestinal cancer cells HCT-15 and SW620 and glioma cells C6 and induce apoptosis and necrosis of the cancer cells, and can be applied to the preparation of medicines for treating malignant tumors, wherein the chemical structure of the fradimycin B is as shown in the specification.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a new biologically active substance, fradimycin B (Fradimycin B), a preparation method thereof, and an application in the preparation of antibacterial and antitumor drugs. Background technique [0002] Infectious diseases still seriously endanger human health and life, and more than 17 million people die of infectious diseases every year in the world. Staphylococcus aureus ( Staphyloccocus aureus ) is an important pathogen of humans, causing many serious infections. Moreover, with the widespread use of antibiotics, the emergence of drug-resistant pathogenic bacteria, especially super-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), has become a Nosocomial and community infections are more difficult and important issues. my country is one of the countries that use the most antibiotics in the world, and the harm of dr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D407/12C12P17/16A61P31/04A61P35/00C12R1/54
Inventor 张治针连晓媛辛文秀叶雪威虞斯然
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap