Pest control method

A technology for pests and Spodoptera frugiperda, applied in the field of pest control

Active Publication Date: 2013-03-27
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It has been proved that plants transgenic for Cry1Ab gene can resist the attack of Lepidoptera pests such as corn borer, cotton bollworm, and rice stem borer. Reports on Plant Hazards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0078] The first embodiment, the acquisition and synthesis of Cry1Ab gene

[0079] 1. Obtain the Cry1Ab nucleotide sequence

[0080] The amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Ab-01 encoding the amino acid sequence (818 amino acids) corresponding to the Cry1Ab-01 insecticidal protein Nucleotide sequence (2457 nucleotides), as shown in SEQ ID NO:3 in the sequence listing.

[0081] The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Cry1Ab-02 encoding the amino acid sequence (615 amino acids) corresponding to the Cry1Ab-02 insecticidal protein Nucleotide sequence (1848 nucleotides), as shown in SEQ ID NO:4 in the sequence listing.

[0082] 2. Synthesis of the above Cry1Ab nucleotide sequence

[0083] The Cry1Ab-01 nucleotide sequence (as shown in SEQ ID NO: 3 in the sequence listing) and the Cry1Ab-02 nucleo...

no. 2 example

[0084] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0085] 1. Construction of a recombinant cloning vector containing the Cry1Ab gene

[0086] The synthesized Cry1Ab-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Ab-01 is the Cry1Ab-01 nucleotide sequence (SEQ ID NO: 3); MCS is the multiple cloning site).

[0087] Then, the recombinant cloning vector DBN01-T was transformed into Escherichi...

no. 3 example

[0096] The third embodiment, the acquisition and verification of corn plants transferred to Cry1Ab gene

[0097] 1. Obtaining corn plants transferred to the Cry1Ab gene

[0098] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the The T-DNA in recombinant expression vectors DBN100124 and DBN100106 (including the promoter sequence of maize Ubiquitin gene, Cry1Ab-01 nucleotide sequence, Cry1Ab-02 nucleotide sequence, PMI gene and Nos terminator sequence) were transferred to maize chromosome In the group, corn plants transformed with the Cry1Ab-01 nucleotide sequence and corn plants transformed with the Cry1Ab-02 nucleotide sequence were obtained; meanwhile, wild-type corn plants were used as controls.

[0099] For Agrobacterium-mediated transformation of maize, briefly, immature immature embryos are isol...

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PUM

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Abstract

The invention relates to a method for controlling athetis lepigone pest, which comprises a step of enabling contact between athetis lepigone pest and Cry1A protein. According to the invention, Cry1A protein capable of killing athetis lepigone is generated in a plant body to control the athetis lepigone pest. Compared with the agricultural control method and chemical control method of the prior art, the method provided by the invention protects the full child-bearing period and full plant to control the invasion of the athetis lepigone pest, avoids pollution and residue, realizes a stable and thorough effect, and is simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1A protein expressed in plants to control the damage of plants by Spodoptera spp. Background technique [0002] Athetis lepigone belongs to Lepidoptera Noctuidae and is an omnivorous pest. At present, it is found that it mainly harms corn crops. It is mainly distributed in the summer corn planting areas of Huanghuaihai in my country, and it is mainly distributed in Japan, North Korea, Russia, Europe and other places. The two-spot moth mainly harms the corn roots at the soil surface of the aerial roots of corn, and bites off the corn stems or superficial roots of the corn. A large area of ​​blank land appears, seriously endangering plots and even destroying species. [0003] Corn is the main food crop in China. On July 9, 2011, CCTV News Network reported for the first time the occurrence of Noctuid moth in China. From the autumn of 2011 to May 31, 2012, the ...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01P7/04A01N63/50
CPCA01N63/02C07K14/325C12N15/8286A01N37/46A01H5/00Y02A40/146A01N63/50A01N63/23
Inventor 康越景庞洁张爱红程鹏杨旭牛丽红贾志伟安罗旭田康乐姜自芹
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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