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Cell culture vessel and cell culture device

A cell culture and container technology, applied in the field of cell culture containers and cell culture devices, can solve the problems of multi-labor and cost, biological pollution, difficult double-layer culture, etc., and achieve the effect of safe recovery and inhibition of invasion.

Inactive Publication Date: 2013-03-20
HITACHI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technical problems here are: since the cell culture is performed by the hands of technicians, it takes a lot of labor and cost to prepare cells for a patient; and there is a risk of biological contamination caused by manual handling
However, the closed-system cell culture devices developed so far have a device configuration corresponding to a cell culture container with a single culture layer, so it is difficult to perform double-layer culture.

Method used

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  • Cell culture vessel and cell culture device
  • Cell culture vessel and cell culture device
  • Cell culture vessel and cell culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051]

[0052] figure 1 It is a figure which shows an example of the structure of the cell culture container in 1st Example.

[0053] figure 1 (a) is a plan view, (b) is its A-A sectional view, and (c) is its B-B sectional view. The cell culture container 1 is a square and flat container made of plastics such as polycarbonate, polystyrene, polypropylene, etc. having both plasticity and rigidity. The frame body 2 is formed by injection molding or the like, and round portions 3 , 4 and 5 are formed therein. Furthermore, a gas permeable membrane 6 is welded to the circular portion 3 , a substance permeable membrane 7 is welded to the circular portion 4 , and a gas permeable membrane 8 is welded to the circular portion 5 . For welding the gas permeable membranes 6, 8 and the substance permeable membrane 7 to the circular parts 3, 5, 4, thermal welding or ultrasonic welding without using an adhesive that affects cell survival is preferable. By the above-mentioned layers and ...

specific example 1）

[0077]

[0078] First, made by injection molding figure 1 Frame 2 shown (material polycarbonate). Figure 12 The appearance of the frame body 2 actually produced is shown. By ultrasonic welding, the figure 1 The gas permeable film is welded on the round parts 3 and 5 of the figure 1 The material permeable membrane is welded on the circular part 4 (ultrasonic welding machine uses 2000Xdt40 manufactured by Branson Company: 0.8). deposited on figure 1The membranes of the circles 4 and 5 were hydrophilized by a plasma device (PC-300 manufactured by Samco was used for the plasma device). Figure 13 It shows the appearance of the state where the culture solution is kept in the container 1 after each film is welded.

[0079]

[0080] Next, a method for culturing corneal epithelial cells using the prepared cell culture container will be described. On the day before culturing corneal epithelial cells, as feeder cells, NIH-3T3 cells were treated with Mitomycin C (10 μg / ml) at ...

specific example 2）

[0097]

[0098] A temperature-responsive culture surface is produced by electron beam polymerization of N-isopropylacrylamide, which is a temperature-responsive high molecular weight monomer, on the cell culture surface of a closed system culture container. After confirming that the adhesion and detachment of corneal epithelial cells were normally carried out on the culture surface, corneal epithelial tissues were produced in the same manner as in Example 1.

[0099]

[0100] On the 16th day of culture, replace with fresh culture medium at room temperature, and let stand at room temperature (about 25°C) for 30 minutes. Then, using a ring-cut hydrophilic PVDF membrane (manufactured by Millipore) as a supporting membrane, the cell sheet was peeled off from the culture surface and recovered.

[0101]

[0102] It carried out similarly to specific example 1.

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Abstract

Disclosed is a cell culture vessel that can prevent liquid leakage from the cell culture space and entry of particles containing microorganisms from outside the cell culture space, and that can prepare regenerated tissue in a manner that is safe and results in peace of mind. The cell culture vessel is mounted to a cell culture device, holds / cultures cells, and is characterized by being provided with: a culture solution holding section that holds a culture solution, a protruding structure section that is for supplying and discharging the aforementioned culture solution; and a culture solution duct that passes from the aforementioned protruding structure section to the aforementioned culture solution holding section.

Description

technical field [0001] The present invention relates to a cell culture container and a cell culture device using the cell culture container. Background technique [0002] In regenerative medicine for treating diseases using one's own cells or cells of others, cells collected from a living body are usually cultured to increase the number of cells or formed into tissues in an appropriate shape for treatment. Cultivation of cells used for treatment needs to be carried out in accordance with GMP (Good Manufacturing Practice: Good Manufacturing Practice) in a clean room for cell culture called a cell processing center (CPC). The technical problems here are: since cell culture is performed by the hands of technicians, it takes a lot of labor and cost to prepare cells for one patient; and there is a risk of biological contamination caused by manual handling. [0003] As a means to solve these technical problems, a device for automating the cell culture process in a closed system h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M29/10C12M37/00C12M25/04C12M23/10
Inventor 中岛亮太守谷腾小林丰茂松冈志津野崎贵之森圭祐
Owner HITACHI LTD
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