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Method for producing Gamma-aminobutyric acid through centrifugal mother liquid of glutamic acid

A technology of aminobutyric acid and centrifugal mother liquor, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem that the filter membrane suction filtration precision is not high, it is not suitable for continuous operation and industrial production, Complex separation and purification steps, etc., to achieve the effect of easy cleaning and maintenance, realizing resource utilization, and reducing the discharge of bacterial residues

Active Publication Date: 2013-03-20
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The separation and purification steps of this process are complicated, and the precision of filter paper filtration and 0.45μm filter membrane suction filtration is not high, so it is not suitable for continuous operation and industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The lactic acid bacteria liquid was inoculated in the culture medium MRS and cultured for 24 hours (1 volume of lactobacillus liquid: 200 volumes of culture medium) to obtain the seed culture liquid. Then the activated lactic acid bacteria seed culture solution is expanded and cultivated and then enters the ceramic membrane for separation and recovery. The ceramic membrane has a pore size of 500nm, an operating pressure of 0.1MPa, and a membrane surface flow rate of 0.5m / s. After concentration, it will be separated and recovered through the ceramic membrane. The strain is added to the monosodium glutamate isoelectric crystallization mother liquor, and fermented to obtain a glutamic acid fermentation broth, and the fermentation temperature is 20°C.

[0035] The fermented liquid is passed through the ceramic membrane to remove large particles of impurities and bacteria. The ceramic membrane has a pore size of 50nm, an operating pressure of 0.3 MPa, and a membrane surface f...

Embodiment 2

[0041] The lactobacillus activation method is the same as in Example 1. Then the activated lactic acid bacteria seed culture solution is expanded and cultivated and then enters the ceramic membrane for separation and recovery. The ceramic membrane has a pore size of 500nm, an operating pressure of 0.1MPa, and a membrane surface flow rate of 0.5m / s. After concentration, it will be separated and recovered through the ceramic membrane. The strain is added to the monosodium glutamate isoelectric crystallization mother liquor, and fermented to obtain a glutamic acid fermentation liquid, and the fermentation temperature is 20°C.

[0042] The fermented liquid is passed through a ceramic membrane to remove large particles of impurities and bacteria. The pore size of the ceramic membrane is 20 nm, the operating pressure is 0.1 MPa, and the membrane surface flow rate is 1 m / s. After the concentration is 10 times, water dialysis is started, and the dialyzed water volume is the ceramic mem...

Embodiment 3

[0047] The lactobacillus activation method is the same as in Example 1. Then the activated lactic acid bacteria seed culture solution is expanded and cultivated and then enters the ceramic membrane for separation and recovery. The ceramic membrane has a pore size of 500nm, an operating pressure of 0.1MPa, and a membrane surface flow rate of 0.5m / s. After concentration, it will be separated and recovered through the ceramic membrane. The strain is added to the monosodium glutamate isoelectric crystallization mother liquor, and fermented to obtain a glutamic acid fermentation liquid, and the fermentation temperature is 20°C.

[0048] The fermented liquid is passed through a ceramic membrane to remove large particles of impurities and bacteria. The ceramic membrane has a pore size of 500nm, an operating pressure of 0.5 MPa, and a membrane surface flow rate of 6 m / s. After concentration of 30 times, water dialysis is started, and the amount of dialyzed water is the ceramic membrane...

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Abstract

The invention relates to a production method of Gamma-aminobutyric acid. The method comprises the following steps: (1) fermentation of monosodium glutamate isoelectric mother liquor: inoculating lactic acid bacteria strain to the monosodium glutamate mother liquor which is subjected to isoelectric crystallization, and fermenting to obtain the fermentation liquor of Gamma-aminobutyric acid; (2) removal of impurities: processing the fermentation liquor of the Gamma-aminobutyric acid through a ceramic membrane filter so as to remove larger particulate impurities and thallus, and concentrating, then adding dialysis water for dialyzing, so as to obtain the clear liquid of the ceramic membrane; (3) decolorizing and removing of micromolecular impurities: filtering the clear liquid of the ceramic membrane obtained in step (2) through a nanofiltration membrane, and then carrying out dialysis to obtain the clear liquid of the nanofiltration membrane; and (4) concentrating: concentrating the clear liquid subjected to the nanofiltration and dialyzing and obtained in step (3) through a reverse osmosis membrance, thus obtaining the concentrate of the reverse osmosis membrance, and finally drying to obtain the Gamma-aminobutyric acid. According to the method, the supernate obtained after the isoelectric extraction of glutamic acid in the monosodium glutamate production technology is recycled to produce the Gamma-aminobutyric acid; and the method is easy for industrial continuity, low in energy consumption, and high in purity of the Gamma-aminobutyric acid.

Description

technical field [0001] The invention relates to a production method of gamma-aminobutyric acid, in particular to a method for purifying gamma-aminobutyric acid in monosodium glutamate mother liquor fermentation liquid based on membrane filtration separation technology, belonging to the field of amino acid production. [0002] Background technique [0003] Monosodium glutamate, also known as MSG L-glutamic acid monosodium salt, contains a molecule of crystal water and has a strong umami taste. It is the most used condiment in the world. The supernatant after the isoelectric extraction of glutamic acid in the MSG production process is called the isoelectric mother liquor. The pH of the isoelectric mother liquor is about 3.0. %~4%, some even as high as 7%~9%. If such a high content of glutamic acid waste liquid is discharged directly, it will cause serious waste of resources and environmental pollution. Therefore, it is necessary to carry out comprehensive treatment of this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07C229/08C07C227/40C12R1/225
Inventor 彭文博赵艳秦泗光陈磊熊福军
Owner JIANGSU JIUWU HITECH
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