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Bivalent vaccine for porcine reproductive and respiratory syndrome and classical swine fever prevention or treatment, and preparation method thereof

A technology for porcine blue-ear disease and dual vaccine, which is applied to medical preparations containing active ingredients, antiviral agents, pharmaceutical formulations, etc., can solve the problems of large immunization workload and high cost, and achieves high titer content and preparation method. The effect of simple, economical immunization procedures

Active Publication Date: 2014-08-27
PU LIKE BIO ENG
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] At present, there are only monovalent vaccines for porcine blue ear disease and swine fever, which need to be injected separately. Not only is the immunization workload heavy and the cost is high, but also multiple immunizations cause greater stress on the pig body, requiring interval immunizations

Method used

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  • Bivalent vaccine for porcine reproductive and respiratory syndrome and classical swine fever prevention or treatment, and preparation method thereof
  • Bivalent vaccine for porcine reproductive and respiratory syndrome and classical swine fever prevention or treatment, and preparation method thereof
  • Bivalent vaccine for porcine reproductive and respiratory syndrome and classical swine fever prevention or treatment, and preparation method thereof

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Embodiment 1

[0023] Embodiment 1, the preparation of porcine blue-ear disease and swine fever dual live vaccine

[0024] 1. Preparation of poisonous seeds for production

[0025] Preparation of porcine blue-ear virus species: get well-grown Marc-145 cells (purchased from China Veterinary Drug Administration), digest with trypsin, and inoculate in cell bottles, and simultaneously use virus species with 1% v / v fetal MEM (manufactured by Invitrogen) maintenance solution of bovine serum (manufactured by PAA) was diluted 10 times, inoculated with virus according to 5% volume inoculum, cultured at 37°C, and observed for cytopathic changes (CPE) every day, when more than 70% of the cells showed CPE Harvest the virus at the same time, freeze and thaw 3 times, harvest the virus, the virus titer is 10 per 1ml virus content 9.5 TCID 50 .

[0026] The preparation of classical swine fever virus kind: use the α-MEM (Invitrogen company production) cell maintenance fluid that contains 1%v / v fetal calf ...

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Abstract

The present invention provides a bivalent vaccine for porcine reproductive and respiratory syndrome (PRRS) and classical swine fever (CSF) prevention or treatment. The bivalent vaccine comprises the following components: PRRS antigen with a content of at least 10<5>TCID50 / unit, and CSF antigen with a content of at least 7500 rabbit infection dose / unit. The bivalent vaccine preparation method comprises: adopting passage cells to culture PRRS viruses and CSF viruses to obtain PRRS antigens and CSF antigens, mixing the two antigen components according to a certain ratio, and auxiliarily adopting a freeze drying protection agent to prepare the freeze drying vaccine. According to the present invention, the two antigens achieve the appropriate ratio, such that the bivalent vaccine achieves or exceeds efficacy of the original single vaccine; the bivalent vaccine has characteristics of simple preparation method, high titer content and convenient and rapid immunization; compared with fractionated immunization, vaccines for PRRS and CSF prevention and treatment through immunization at least 2 times or 3 times, and immunization methods thereof in the prior art, the bivalent vaccine and the immunization method thereof have characteristics of reduced immunization cost, saved immunization program, economy and reliability.

Description

technical field [0001] The invention relates to a dual vaccine for preventing or treating pig blue-ear disease and swine fever and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Pig blue ear disease, also known as porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, referred to as PRRS), is a kind of viral infectious disease that endangers the pig industry. It is characterized by reproductive disorders such as fetuses, respiratory diseases and high mortality in pigs of all ages. In the summer and autumn of 2006, pig "high fever" epidemics occurred in some provinces in southern my country. The Ministry of Agriculture actively organized scientific research personnel to jointly tackle the problem, and identified the pig PRRS variant virus as the main pathogen, which was named "highly pathogenic pig PRRS". Until today, the epidemic situation is still severe. Like other viral dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/14A61K39/12A61K39/187
Inventor 张许科孙进忠
Owner PU LIKE BIO ENG
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