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Neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit

A lipocalin and chemiluminescence detection technology, applied in the field of immunoassay medicine, can solve the problems of ELISA method, such as many affected factors, poor detection limit, poor accuracy, etc., and achieve good specificity, simple operation, reliability and accuracy. high degree of effect

Inactive Publication Date: 2013-03-13
天津市协和医药科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit developed by the US company Boshi uses double-antibody sandwich immunofluorescence chromatography, but its detection limit is lower than that of the ELISA method, and the ELISA method is also affected by many factors and has poor accuracy.

Method used

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  • Neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit
  • Neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0043] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0044] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0045] ③Magnetic particles coated with NGAL monoclonal antibody;

[0046] ④ White opaque microwell plate

[0047] ⑤ washing liquid;

[0048] ⑥ Chemiluminescent substrates A and B;

[0049] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0050] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0051] ②W...

Embodiment 2

[0073] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0074] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0075] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0076] ③Magnetic particles coated with NGAL monoclonal antibody;

[0077] ④ White opaque microwell plate;

[0078] ⑤ washing liquid;

[0079] ⑥ Chemiluminescent substrates A and B;

[0080] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0081] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0082] ②...

Embodiment 3

[0103] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0104] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0105] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0106] ③Magnetic particles coated with NGAL monoclonal antibody;

[0107] ④ White opaque microwell plate;

[0108] ⑤ washing liquid;

[0109] ⑥ Chemiluminescent substrates A and B;

[0110] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0111] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0112] ②...

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Abstract

The invention discloses a neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit. The kit comprises the components of a standard substance of NGAL, an NGAL monoclonal antibody labeled by horseradish peroxidase, magnetic particles coated by the NGAL monoclonal antibody, a white non-transparent microwell plate, washing liquid, and chemiluminescence substrates A and B. The kit provided by the invention can be used for detecting the content of the NGAL in blood serum of a patient, and has important guiding significance to the detections on premature kidney diseases and injures. Compared with the conventional ELISA (Enzyme Linked Immunosorbent Assay) method, the NGAL chemiluminescence detection kit provided by the invention has the characteristics of being high in sensitivity and good in specificity and overcoming the large influence of immunoturbidimetry by blood fat concentration, and has the advantages of high stability, reliability and accuracy, security and environment friendliness, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention belongs to the technical field of immunoanalysis medicine, and in particular relates to a neutrophil gelatinase-associated lipocalin chemiluminescent detection kit and a preparation method. Background technique [0002] Neutrophil gelatinase associated lipocalin (NGAL), also known as human lipocalin 2 (lipocalin2, Ln2) or siderocalin (siderocalin), is a member of the human lipocalin family member. Under physiological conditions, neutrophils, renal tubular epithelial cells, alveolar macrophages, and bronchial epithelial mucus cells secrete a small amount of NGAL, while NGAL in tissue cells such as brain and peripheral nerves, colon, uterus and ovary, placenta, and thyroid expression was negative. The concentration range of NGAL in healthy volunteers is approximately, urine: 0.7-9.6 ng / mL; plasma: 34-106 ng / mL. According to the 90% positive predictive value of patients with acute kidney injury (AKI), the positive judgment value of NGAL i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/76
Inventor 曹青潘学继王立凯单存海洪宇霞
Owner 天津市协和医药科技集团有限公司
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