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Paddy Hd-q gene and application thereof in cultivation of paddy with wide applicability

A gene and rice technology, applied in the field of plant breeding, can solve the problems affecting rice yield and the complex way of rice yield

Active Publication Date: 2013-11-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the way of affecting rice yield is complex, and different traits and different genetic pathways may affect rice yield

Method used

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  • Paddy Hd-q gene and application thereof in cultivation of paddy with wide applicability
  • Paddy Hd-q gene and application thereof in cultivation of paddy with wide applicability
  • Paddy Hd-q gene and application thereof in cultivation of paddy with wide applicability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Cloning and functional verification of embodiment 1Hd-q gene

[0017] In the previous study, we used the japonica rice variety Nipponbare as the recipient parent, and the indica rice varieties Qingluzhan 11 and Longtefu (provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences, see China Crop Germplasm Information Network http: / / icgr .caas.net.cn / ) etc. as donor parents, constructed a series of chromosome segment replacement lines. A replacement line C63 was found in the progeny of Qingluzhan 11, which showed delayed heading phenomenon under long-day sunshine conditions in Yangzhou summer or short-day sunshine conditions in Hainan winter. By crossing C63 with Nipponbare, F1 heterozygous plants were obtained, and the F1 plants showed normal heading. Genetic analysis was carried out on the F2 segregation population derived from F1 plants and the gene was initially located. Some plants were randomly selected from the F2 population, and it was fo...

Embodiment 2

[0021] Embodiment 2 Design and detection of dcaps1 molecular marker

[0022] According to Example 1, compared with the EF7 gene, there is a special single nucleotide variation site (hereinafter referred to as the SNP site) on the second exon of the Hd-q gene, specifically in the Hd-q gene The base at the 395th position of the coding sequence is A, and the corresponding position in EF7 is T. As a result, the Hd-q gene forms a stop codon at this position. According to this SNP, the specific dCAPS marker dcaps1 (upstream primer dcaps1-FSEQ ID NO.2 and downstream primer dcaps1-R SEQ ID NO.3) is used to find and verify this special site ( figure 1 ), the restriction endonuclease HpaI used can recognize the rice of the genotype of T at the SNP site (such as Nipponbare, called TT genotype), but cannot recognize the rice of the genotype of mutated base A (such as Qing Lu accounted for 11, known as the AA genotype).

[0023] The total rice DNA was amplified by PCR with primer dcaps1 ...

Embodiment 3

[0024] Example 3 Molecular marker-assisted breeding of new rice lines containing Hd-q gene

[0025] The present invention provides a way of thinking to use rice plants containing Hd-q genes (such as rice variety Qingluzhan 11) as donor parents, and rice varieties or strains to be improved (such as Nipponbare) as recurrent parents to carry out continuous backcross selection. education. In the low generation, the molecular marker dcaps1 linked with the target gene Hd-q gene and its delayed heading target traits in Example 2 is used for assisted selection, and can be selected in conjunction with the heading stage phenotype in the high generation population, after a certain period of time After one generation, new varieties or lines can be bred that not only retain the excellent traits of the original parent to be improved, but also have good regional adaptability.

[0026] The invention introduces the Hd-q gene into a japonica rice variety Nipponbare that has completed the whole...

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Abstract

The invention discloses a gene Hd-q for controlling the growing period of the base nutrition of the paddy and a method for cultivating the paddy variety which is wide in geographical adaptability by using the gene. The Hd-q gene is a new allele of the paddy EF7 gene, and contains a specific mononucleotide variation point, so that the Hd-q allele-containing paddy variety is insensitive to illumination and wide in applicability. Compared with the coded sequence of the EF7 gene, and the coded sequence of the gene Hd-q in the growth period is characterized in that the basic group at the 395th site has the mutation from T to A to form into a termination codon. According to the T / A single-basic group mutation, a specific dCAPS (derived cleaved amplified polymorphic sequence) is designed to identify the Hd-q gene, and is used for introducing the Hd-q gene into a target variety by means of assistant selection in combination with a conventional hybridization and backcross method to cultivate the paddy new variety which is insensitive to illumination and wide in applicability.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a technology for identifying a gene controlling the basic vegetative growth period of rice and a method for using the gene to cultivate rice varieties with wide regional adaptability. Background technique [0002] In the context of global population increase and environmental degradation, ensuring high and stable yields of major crops such as rice has received more and more attention. It has become one of the most important ways to further improve the high yield and stability of crops by finding those genes involved in yield and growth period traits. [0003] Rice yield traits, such as effective panicle, panicle length, and thousand-grain weight, are generally considered to be controlled by a large number of minor genes. In recent years, through methods such as quantitative trait loci and map-based cloning, several important genes controlling rice yield have be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68A01H5/00
Inventor 刘巧泉赵冬生张昌泉顾铭洪
Owner YANGZHOU UNIV
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