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Method for preparing porous compound type high permeability absorption hemostasis coating with modified nano-crystalline cellulose

A technology of nanocellulose and hemostatic dressing, applied in the field of biomedical materials or medical composite materials, can solve the problems of unsatisfactory and poor wound healing promotion effect, bacterial cellulose has no antibacterial and hemostatic properties, etc., and achieve excellent water absorption and water retention. Performance, Proliferation Promotion, Excellent Biocompatibility and Cell Affinity Effects

Active Publication Date: 2014-03-26
北京中杰瑞康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacterial cellulose itself has no antibacterial properties and has poor hemostatic properties, so the promotion effect on wound healing is not ideal.

Method used

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  • Method for preparing porous compound type high permeability absorption hemostasis coating with modified nano-crystalline cellulose
  • Method for preparing porous compound type high permeability absorption hemostasis coating with modified nano-crystalline cellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Step 1: Take the bacterial cellulose membrane 10×20 cm prepared by Acetobacter xylinum and wash it with deionized water several times to remove the culture medium and impurities on the surface of the membrane. Then soak the membrane in 0.1mol / L NaOH solution, ultrasonically treat it in a cell pulverizer for 15 minutes, and then put it in a water bath at 90°C for 60 minutes to remove the bacteria and residual medium left in the nanofiber network. Run up and milky white translucent. Then rinse with deionized water several times, and measure the pH value with an acidity meter, which is about 7.0 to 8.0, to obtain a purified bacterial cellulose membrane for removing endotoxin;

[0039] Step 2. Soak the bacterial cellulose membrane pretreated in step 1 in 0.01mol / L NaOH solution, place it under a high-speed disperser and disperse it into fine fibers of 800nm ​​to 400μm, so that the fibers are semi-coagulated in the aqueous solution colloidal state, and then 1000r / min low-sp...

Embodiment 2

[0050] Step 1: Take the bacterial cellulose membrane 10×20 cm prepared by Acetobacter xylinum and wash it with deionized water several times to remove the culture medium and impurities on the surface of the membrane. Then soak the membrane in 0.1mol / L NaOH solution, ultrasonically treat it in a cell pulverizer for 20 minutes, and then bathe in water at 100°C for 60 minutes to remove the bacteria and residual medium left in the nanofiber network. Run up and milky white translucent. Then rinse with deionized water several times, and measure the pH value with an acidity meter, which is about 7.0 to 8.0, to obtain a purified bacterial cellulose membrane for removing endotoxin;

[0051] Step 2. Soak the bacterial cellulose membrane pretreated in step 1 in 0.05mol / L NaOH solution, place it in a high-speed disperser and disperse it into fine fibers of 800nm ​​to 400μm, so that the fibers are semi-coagulated in the aqueous solution Colloidal state, and then 1200r / min low-speed centri...

Embodiment 3

[0062] Step 1: Take the bacterial cellulose membrane 10×20 cm prepared by Acetobacter xylinum and wash it with deionized water several times to remove the culture medium and impurities on the surface of the membrane. Then soak the membrane in 0.1mol / L NaOH solution, ultrasonically treat it in a cell pulverizer for 18 minutes, and then bathe in water at 90°C for 60 minutes to remove the bacteria and residual medium left in the nanofiber network. Run up and milky white translucent. Then rinse with deionized water several times, and measure the pH value with an acidity meter, which is about 7.0 to 8.0, to obtain a purified bacterial cellulose membrane for removing endotoxin;

[0063] Step 2. Soak the pretreated bacterial cellulose membrane in step 1 in 0.02mol / L NaOH solution, and place it under a high-speed disperser to disperse into fine fibers of 800nm ​​to 400μm, so that the fibers are semi-coagulated in the aqueous solution. Colloidal state, and then 900r / min low-speed cent...

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Abstract

The invention discloses a method for preparing porous compound type high permeability absorption hemostasis coating with modified nano-crystalline cellulose, and belongs to the field of biological medical materials. The method comprises the following steps of: pre-processing bacterial cellulose by alkali water at high temperature to remove endotoxin; scattering the obtained bacterial cellulose under a high-speed bacterial pulverizer to be fine fibers; performing alkalization treatment on the fine fibers by alkali liquid with the proper concentration to activate the cellulose; adding an etherifying agent grafted modified bacterial cellulose to ensure that the fiber has a certain hemostasis function; uniformly compounding the modified bacterial cellulose with sodium alga acid to form gel network by ion crosslinking; and freezing and drying the gel network to obtain the hemostasis compound material with good performance. The porous compound material prepared by the method is a good medicine carrier, which can carry the medicines capable of resisting bacteria, stopping the pain and coagulating blood, so that the material has good medicine sustained release effect under different PH values. The porous compound material is high in biocompatibility and cell affinity, can promote proliferation and differentiation of stem cells, and facilitates rapid recovery of damaged tissues.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials or medical composite materials, and provides a preparation method of a porous composite high-permeability hemostatic dressing based on modified nanofibers. technical background [0002] Hemorrhage in the human body is very common in various emergency accidents, and there will also be a lot of hemorrhage during surgery. Hemostasis is the most critical link in saving lives. Whether bleeding wounds can be quickly and effectively stopped in a short period of time is a current research hotspot. Therefore, in recent years, medical absorbable hemostatic materials have attracted great attention from the medical and industrial circles of various countries. Many large pharmaceutical companies are trying to develop their own new hemostatic materials, such as the Taehocomd absorbable wound hemostasis developed by Austrian HafsLund Nycomed Pharmaceutical Company. Mounting agent, Gelfix hemostatic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/42A61L15/28
Inventor 郑裕东林清华王红樊卫安家新
Owner 北京中杰瑞康科技有限公司
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