Breeding method for inducing parthenogenesis of corn by propyzamide
A technology of propargin and parthenogenesis, applied in the breeding field of corn inbred lines, can solve the problems of difficult operation, high cost, low doubling rate and the like, and achieve the effects of easy operation, simple method and simple procedure
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Embodiment 1
[0026] (1) Preparation of inducer and selection of processing materials
[0027] Choose 50% propargyl acetamide wettable powder on the market, and dilute with water to an inducer with a concentration of 40 μmol / L.
[0028] The processing object is the corn hybrid "Liaodan 565".
[0029] (2) Induction treatment
[0030] Strictly bag the ears before spinning. When most of the filaments on the ears are spit out, cut the filaments short, leaving about 2.5 cm in length, and then spray the filaments with a small sprayer and put them back in the paper bag. Repeated spraying twice a day at 6:00 and 8:00, for 2 consecutive days. Treat 30 spikes.
[0031] (3) Harvest
[0032] Harvest the ears at maturity, carefully peel off the bracts and remove the seeds.
[0033] (4) Identification and reproduction
[0034] In the next season, the seeds will be sown with a single seed, and individual plants that do not meet the breeding goals will be eliminated, bagged and selfed, and 100 ears will be obtained;...
Embodiment 2
[0036] (1) Preparation of inducer and selection of processing materials
[0037] Use the commercially available propargyl propargyl and dilute with water to an inducer with a concentration of 60 μmol / L.
[0038] The processing object is the corn hybrid "Zhengdan 958".
[0039] (2) Induction treatment
[0040] Strictly bag the ears before spinning. When most of the filaments on the ears are spit out, cut the filaments to a length of about 2.0 cm, and then spray the filaments with a small sprayer, and finally put them back into the paper bag. Spraying was repeated 3 times at 6:00, 8:00, and 17:00, respectively, for 2 consecutive days. Process 40 spikes.
[0041] (3) Harvest
[0042] Harvest the ears after maturity, carefully peel off the bracts, remove the seeds, and obtain 180 seeds.
[0043] (4) Identification and reproduction
[0044] In the next season, the seeds will be sown with a single seed, and individual plants with good phenotypes will be selected, bagged and selfed, and 150 ea...
Embodiment 3
[0047] (1) Preparation of inducer and selection of processing materials
[0048] Use the commercially available propargyl dimethochlor and dilute with water to an inducer with a concentration of 80 μmol / L.
[0049] The processing object is "Jundan 20".
[0050] (2) Induction treatment
[0051] It is planted under the condition of space isolation of 1000 meters, and strictly emasculated before dispersing powder. When most of the filaments on the ears are spit out, and the filament length is about 2.0 cm, spray the filaments with a sprayer. Spraying was repeated 3 times at 6:00, 8:00, and 16:00 for 2 consecutive days. Process 50 spikes.
[0052] (3) Harvest
[0053] Harvest the ears after maturity, carefully peel off the bracts, remove the seeds, and harvest 200 seeds.
[0054] (4) Identification and reproduction
[0055] In the next season, the seeds will be sown with a single seed, 150 excellent individual plants will be selected according to the phenotype, bagged and selfed, and 100 ea...
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