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Prebiotic peptide biological feed additive and preparation method and application thereof

A biological feed and additive technology, applied in animal feed, animal feed, applications, etc., can solve the problems of not being able to obtain production status, and achieve the effect of increasing feed intake and improving the microenvironment

Active Publication Date: 2013-03-06
大庆仕合源乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, animals are not optimally productive when fed homozygous diets formulated with an ideal amino acid profile or low-protein amino acid balanced diets

Method used

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  • Prebiotic peptide biological feed additive and preparation method and application thereof
  • Prebiotic peptide biological feed additive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 fermentation medium

[0036] 5.0g soybean peptone; 5.0g glucose; 5.0g yeast extract powder; 4.0g potassium dihydrogen phosphate; 1mL 3.08% (w / w) manganese sulfate solution, 1000ml deionized water, pH 6.50. .

Embodiment 2

[0037] The preparation of embodiment 2 seed liquid

[0038] 1. Purchase source of the strains involved: All strains were purchased from the China Industrial Microbiology Culture Collection Center, and the specific numbers of the strains are:

[0039] Bacillus subtilis CICC 10066, Bacillus licheniformis CICC 10037, Bacillus natto CICC10260, Saccharomyces cerevisiae CICC 1562, Candida utilis CICC 1314, Lactobacillus casei CICC 20266, Lactobacillus plantarum CICC 20242, Lactobacillus acidophilus CICC 21000, Streptococcus thermophilus CICC 20364, Lactobacillus bulgaricus CICC 20247, Lactobacillus helveticus CICC 22826.

[0040] 2. Preparation of seed solution

[0041] A: Bacillus subtilis and Bacillus licheniformis seed liquid

[0042] 1) Bacillus culture medium

[0043] 5.0g soybean peptone; 5.0g glucose; 5.0g yeast extract powder; 4.0g potassium dihydrogen phosphate; 1mL 3.08% (w / w) manganese sulfate solution, 1000ml deionized water, pH 6.50. .

[0044] 2) Seed solution prepa...

Embodiment 3

[0061] Example 3 Preparation of Enzymolysis Pig Blood Prebiotic Peptide Biological Feed Additive

[0062] 1) Pre-treatment of pig blood

[0063] Crush the coagulated fresh pig blood, add water at a ratio of 1:10, raise it to 115°C, keep the temperature at a constant temperature for 60 minutes, and keep stirring during the period, then grind it through a colloid mill, adjust the colloid mill to 200 mesh, and use a thick slurry The pump heats the ground pig blood through a plate heat exchanger to 58°C and then pumps it into the enzymolysis tank.

[0064] 2) Enzymolysis

[0065] Add alkaline protease (2000U / g) at a weight ratio of 1:250 between protease and treated pig blood, pH 8.5, constant temperature 50°C, start stirring, aseptically take samples every 4 hours to measure the degree of proteolysis, When the acid-soluble protein content is greater than 70% (w / w), proceed to the next step.

[0066] 3) Warm up to kill enzymes

[0067] Heat the enzymolysis solution to 85°C and...

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Abstract

The invention relates to a prebiotic peptide biological feed additive and a preparation method and application thereof. The prebiotic peptide biological feed additive is prepared by mixing and fermentation of seed solution with one or more of bacillus, yeast and lactic acid bacteria in plant protease hydrolysate. After animal and plant protein is subjected to enzymolysis at constant or changeable temperature, the seed solution with one or more of bacillus, yeast and lactic acid bacteria is added for fermentation according to a certain proportion, and the fermented solution is concentrated and dried to obtain a powdery product. The problems of high content of bitter peptides and poor palatability of enzymolysis processes using acid, alkali and protease can be solved. The immune function of animals can be stimulated by active peptides in the prebiotic peptide biological feed additive, the immunity of the animals can be improved, the intestinal environment of the animals can be improved by probiotics, the resistance of the animals to a variety of diseases can be enhanced, the feed conversion rate can be increased, and the breeding cost can be reduced.

Description

technical field [0001] The invention relates to a prebiotic peptide biological feed additive and its preparation method and application, in particular to a biological feed additive containing active peptides and beneficial bacteria obtained by adding probiotics to the enzymatic hydrolyzate of animal and plant proteins for post-fermentation. It belongs to the field of biological feed. Background technique [0002] Since the 1950s, antibiotics have been used as growth promoters for livestock and poultry in the breeding industry. A large number of studies have shown that while antibiotics eliminate pathogenic bacteria, they also eliminate beneficial microorganisms to the animal body, disrupt the intestinal microecological balance, and cause animals, especially Susceptibility of young livestock and poultry to pathogenic microorganisms. In addition, long-term high-dose feeding of antibiotics can cause antibiotic-resistant bacteria harmful to both humans and animals in animals. A...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 杜鹏李建秋霍贵成
Owner 大庆仕合源乳业有限公司
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