Cdca5 peptides and vaccines including the same
An immunology, HLA-A2 technology, applied in the direction of recombinant DNA technology, medical preparations containing active ingredients, peptides, etc., can solve problems such as growth arrest of lung cancer cell lines
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[0415] Materials and methods
[0416] cell line
[0417] HLA-A*0201 positive B-lymphoblastoid cell line T2 and African green monkey kidney cell line COS7 were purchased from ATCC.
[0418] Candidate selection for peptides derived from CDCA5
[0419] Using binding prediction software "NetMHC 3.0" (www.cbs.dtu.dk / services / NetMHC / ) (Buus et al. (Tissue Antigens., 62:378-84, 2003), Nielsen et al. (Protein Sci., 12:1007-17, 2003, Bioinformatics, 20(9):1388-97, 2004)) predicts 9- and 10-mer peptides derived from CDCA5 that bind the HLA-A*0201 molecule. These peptides were synthesized by BioSynthesis (Lewisville, Texas) according to standard solid phase synthesis and purified by reverse phase high performance liquid chromatography (HPLC). The purity (>90%) and identity of the peptides were determined by analytical HPLC and mass spectrometry, respectively. Peptides were dissolved in dimethyl sulfoxide at 20 mg / ml and stored at -80°C.
[0420] In vitro CTL induction
[04...
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