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Preparation method and application for hydrophilic organic polymer liquid phase monolithic chromatographic column

An integral chromatographic column and polymer technology, which is applied in the field of chromatographic research of organic polymer materials, can solve the problems of limited application optimization range, limited polarity of chromatographic column, narrow range of solvent selection, etc., and achieves low cost, less environmental pollution, and preparation The effect of simple process

Active Publication Date: 2013-02-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on monolithic columns with hydrophilic interaction mechanism, these columns have limited polarity and narrow solvent selection range, and most of them can only be applied to the conditions of high organic phase (>60%), which limits the scope of their application optimization

Method used

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  • Preparation method and application for hydrophilic organic polymer liquid phase monolithic chromatographic column
  • Preparation method and application for hydrophilic organic polymer liquid phase monolithic chromatographic column
  • Preparation method and application for hydrophilic organic polymer liquid phase monolithic chromatographic column

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The monomer 3-[N,N-dimethyl-[2-(2-alkenoyloxy)ethylbutylammonium]propane-1-sulfonic acid inner salt 0.0600g, the crosslinking agent N,N-methylene Base bisacrylamide 0.0106g, porogen (including methanol 0.1128g and water 0.0519g) and initiator azobisisobutyronitrile 0.0010g, prepared into a reaction mixture, ultrasonically degassed and poured into a pretreated quartz capillary Then seal both ends of the quartz capillary and put it in a 60°C water bath to react for 12 hours; after the reaction is completed, connect the quartz capillary to a high-pressure pump, wash off unreacted substances with methanol, and obtain SPDA-co-MBA hydrophilic organic polymer Liquid phase monolithic chromatographic column, the scanning electron microscopy results of the organic polymer in the column are as follows: figure 1 shown.

Embodiment 2

[0028] The monomer 3-[N,N-dimethyl-[2-(2-alkenoyloxy)ethylbutylammonium]propane-1-sulfonic acid inner salt 0.0600g, the crosslinking agent N,N-methylene Base bisacrylamide 0.0257g, porogen (methanol 0.1370g and water 0.0630g) and initiator azobisisobutyronitrile 0.0010g, prepared into a reaction mixture, ultrasonically degassed and poured into a pretreated quartz capillary , and then seal both ends of the quartz capillary, and put it in a 60°C water bath to react for 12 hours. After the reaction is completed, connect the quartz capillary to a high-pressure pump, rinse off unreacted substances with methanol, and obtain SPDA-co-MBA hydrophilic organic polymer Liquid chromatography monolithic columns.

Embodiment 3

[0030] The monomer 3-[N,N-dimethyl-[2-(2-alkenoyloxy)ethylbutylammonium]propane-1-sulfonic acid inner salt 0.0600g, the crosslinking agent N,N-methylene Base bisacrylamide 0.0132g, porogen (methanol 0.1170g and water 0.0538g) and initiator azobisisobutyronitrile 0.0010g, prepared into a reaction mixture, ultrasonic degassed and poured into the pretreated quartz capillary , and then seal both ends of the quartz capillary, and put it in a 60°C water bath to react for 12 hours. After the reaction, connect the quartz capillary to a high-pressure pump, and rinse off unreacted substances with acetone to obtain SPDA-co-MBA hydrophilic organic polymer Liquid chromatography monolithic columns.

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Abstract

The invention belongs to the research field of organic polymeric materials, and discloses a preparation method and an application for a hydrophilic organic polymer liquid phase monolithic chromatographic column. The method comprises the following steps of employing monomers of 3-[N,N-dimethyl-[2-(2-enoyloxy)ethyl]butylamin]propane-1-sulfonate as functional monomers, mixing the functional monomers with a cross-linking agent, a pore-forming agent and an initiator, performing a thermocatalytic reaction, and carrying out in-situ polymerization in a quartz capillary to form the polymer liquid phase monolithic chromatographic column. The obtained chromatographic column has larger hydrophilic interaction area than a common hydrophilic chromatographic column. The monolithic chromatographic column is small in usage amount of the organic mobile phase, has little environmental pollution, is simple in preparation process and low in cost, has low column pressure, and is suitable for rapid separation of polar compounds.

Description

technical field [0001] The invention belongs to the field of chromatographic research on organic polymer materials, and in particular relates to a method and application for in-situ preparation of a monolithic column of a hydrophilic organic polymer through thermal polymerization technology. Background technique [0002]As we all know, liquid chromatography and its combined technology have been widely used in the fields of environment, medicine and life sciences, and the research on its core components, chromatographic separation media and analysis functions, is also deepening. In recent years, new monolithic materials for chromatographic stationary phases have become one of the research hotspots due to their advantages of high permeability, low column pressure, fast mass transfer, and simple preparation, and have been successfully used in reversed-phase chromatography, ion-exchange chromatography, Applied in separation modes such as affinity chromatography, it effectively s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/22B01D15/20C07K1/16
Inventor 江正瑾袁广新彭咏波王婷婷洪洁雅肖遥
Owner JINAN UNIVERSITY
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