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Method and detection kit for detecting cervical fluid heme

A detection box and hemoglobin technology, which is applied in the detection of hemoglobin in cervical exudate, and in the field of special detection box for detection of hemoglobin in cervical exudate, can solve the problems of complicated operation, long cycle and high cost, and achieve low cost, high sensitivity, Effect of reducing labor burden

Active Publication Date: 2013-02-13
青岛宏利源高新技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of high cost, long period, and complicated operation in the prior art method for detecting hemoglobin in cervical exudate, the present invention provides a method for detecting hemoglobin in cervical exudate, which has high sensitivity, low cost, and rapid detection of hemoglobin in cervical exudate; at the same time, the invention also provides a special detection box for detecting hemoglobin in cervical exudate

Method used

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  • Method and detection kit for detecting cervical fluid heme
  • Method and detection kit for detecting cervical fluid heme
  • Method and detection kit for detecting cervical fluid heme

Examples

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Effect test

Embodiment 1

[0023] A method for detecting hemoglobin in cervical exudate, the method is as follows: slide open the sliding cover 1 of the detection box, take out the finger cot 6 from the box body 8, put on latex gloves, and then put the finger cot 6 on the first joint of the index finger On the cervix, wipe the cervix with a little pressure to obtain exudate, wherein the amount of exudate is 0.5-1 ml; take the sample cup 7 in the sample cup placement area 3, and put it into tris-hydrochloric acid with a pH value of 7.4 3 ml of buffer solution, put the finger cot 6 with seepage into the sample cup 7, and mix well; take out two droppers from the two strip grooves 2, and the two droppers are pre-filled with reagent A and reagent B, sequentially add 1ml of sample solution, 0.5ml of reagent A, and 0.5ml of reagent B into the test pool 4, mix well, observe the color change within 2 minutes, compare and record the test results with the color guide 5; the preparation of the reagent A The me...

Embodiment 2

[0030] Combine below Figure 1 to Figure 4 The special detection box of the present invention is further introduced.

[0031] The detection box for detecting cervical exudate hemoglobin provided by the present invention includes a box body 8 and a slide cover 1 slidingly connected with the box body 8, the slide cover 1 can slide along the direction of the upper surface of the box body 8, and the slide cover 1 is provided with There are two bar-shaped grooves 2 protruding outward, a dropper is housed in the bar-shaped groove 2, the bar-shaped groove 2 is sealed from the bottom, the inside of the box body 8 is hollow, and one side of the box body 8 is provided with a color comparison plate 5, The inner bottom of the box body 8 is also provided with a test pool 4 and a sample cup placement area 3 for placing the sample cup 7. Finger cots 6 are placed on the 4 sides of the test pool. The test pool 4 extends inward from the bottom of the box body 8, and the middle part is downw...

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Abstract

The invention provides a method for detecting cervical fluid heme. The method comprises the following steps of: wiping at the cervical part with little pressure to obtain the fluid; processing the fluid with a tri-hydroxymethyl aminomethane-hydrochloric acid buffer solution with a pH value of 7.4; and then reacting with a color producing reagent, comparing with a shade guide and recording the detection result. The method provided by the invention is simple and practical and low in cost, and can quickly screen the sample without heme, the sample with heme and the sample possibly with heme in the cervical fluid, thereby providing preparation for further screening. The invention also relates to a detection kit for detecting the cervical fluid heme.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting hemoglobin in cervical exudate, and at the same time, the invention also relates to a special detection box for detecting hemoglobin in cervical exudate. Background technique [0002] The hemoglobin in cervical exudate is often caused by the release of hemoglobin from tissue cell oxygen receptors and FixL transmembrane protein, hemoglobin precipitation from tumor cells, or exudation from microcirculation. Generally, it is necessary to use advanced instruments and analytical chemical methods for detection, which is costly, long-term, and complicated to operate. Therefore, it is an urgent need to invent a simple and low-cost detection method to quickly determine whether hemoglobin is contained in cervical exudate. solved problem. Contents of the invention [0003] In order to solve the problems of high cost, long cycle and complicated operation in the prior art meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N33/72
Inventor 温鑫崔子伦杜晓丽
Owner 青岛宏利源高新技术有限公司
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