Method for preparing iridoid aglycone

Abstract

The invention relates to a method for preparing iridoid aglycone. The method comprises that: iridoid glycoside is subjected to enzyme hydrolysis while continuous liquid-liquid extraction is performed, and an ether organic solvent or an alkyl halide organic solvent is adopted to carry out extraction separation on the hydrolysate iridoid aglycone, such that the high yield iridoid aglycone is obtained.

Description

technical field The invention relates to the field of biotechnology, and relates to a method for preparing aglycone from iridoid glycosides with high yield. Background technique Iridoid compounds widely exist in the plant kingdom, and are most common in Rubiaceae, Wintergreen, and Crystal Orchidaceae. Most of the iridoid components in plants exist in the form of glycosides (Iridoid glycosides), such as Rubiaceae gardenia contains geniposide (gardenoside, C 17 h 24 o 11 ), geniposide (C 17 h 24 o 10 ), genipin-1-β-D-gentiobioside (genipin-gentiobioside), geniposidic acid (geniposidic acid, C 16 h 22 o 10 ), asperuloside (C 18 h 22 o 11 ), Paederoside (paederoside, C 18 h 22 o 11 S), aucubin in plantain (aucubin, C 15 h 22 o 9 )Wait. Most of the iridoid glycosides are white crystals or powder, which have been proved to be easily hydrolyzed, especially in the presence of β-D-glucosidase, and are easily hydrolyzed into the corresponding aglycone cycloalkene et...

AI Technical Summary

Problems solved by technology

But there are following problems in this method: 1, productive rate is lower; 2, still can contain impurities such as the dimer of 2-10% geniposide and genipin in the product of genipin

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