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Application of chitosan to yolk fatty deposition and derosination technology

A technology of chitosan and chitosan solution, applied in the preparation method of peptides, from egg immunoglobulin, organic chemistry, etc., can solve the problems of large amount of degreasing reagents, high loss rate of IgY, difficult to remove, etc. Achieve the effects of low environmental conditions, less IgY loss, and less reagent consumption

Inactive Publication Date: 2013-02-13
梁永利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2009, Deng Bihua (Advances in Veterinary Medicine, 2009,30(1):38-40) compared the fat removal effect of poloxamer and polyethylene glycol, and believed that there was no significant difference between the two, and when the concentration was too high, both would destroy the antibody The hydration film of the protein affects the antibody titer, and the cost of poloxamer is higher than that of polyethylene glycol
In 2011, Zhang Lun et al. (China Animal Quarantine, 2011,28(3):49-51) conducted a study on the degreasing conditions of chicken yolk by poloxamer-octanoic acid method, and found that protein recovery and antibody efficacy increased with the concentration of octanoic acid. prices are gradually reduced
However, the above method has the following problems: using a large amount of inorganic or organic reagents, even toxic organic solvents, the residues of toxic and harmful reagents are difficult to remove; protein is also consumed during degreasing, and the loss rate of IgY is high, which may even lead to antibody inactivation; a large amount of Instruments and equipment increase the cost of degreasing
The Chinese patent with the publication number CN101225103A discloses the technology of degreasing yolk polyethylene glycol by one-time precipitation, which is safe, non-toxic, and does not use large-scale equipment, but the degreasing agent used in this method is polyethylene glycol, which utilizes polyethylene glycol The strong water absorption ability breaks the emulsified state of egg yolk, the amount of degreasing reagent used is relatively large, and the loss rate of IgY is relatively high

Method used

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  • Application of chitosan to yolk fatty deposition and derosination technology
  • Application of chitosan to yolk fatty deposition and derosination technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In an environment of 4-50°C, take the yolk out of the egg, break the yolk membrane, measure 100m1 of the yolk stock solution, and place it in a 500m1 beaker. Accurately weigh 0.4g of chitosan and dissolve it in 100m1 of 1% (V / V) acetic acid solution, stir until completely dissolved to obtain a chitosan solution, then pour the chitosan solution into the egg yolk stock solution and mix well, let stand Precipitation for 24h. The precipitated mixed solution is divided into two layers, the bottom precipitate is egg yolk lipid component, and the supernatant is egg yolk water-soluble component, that is, a mixed solution containing immunoglobulin IgY.

Embodiment 2

[0026] In an environment of 4-50°C, take the yolk out of the egg, break the yolk membrane, measure 100m1 of the yolk stock solution, put it in a 500m1 beaker, add 100m1 distilled water to dilute, mix well, and make the yolk dilution. Accurately weigh 0.6g of chitosan and dissolve it in 100m1 of 1% (V / V) acetic acid solution, and stir until completely dissolved. Pour the chitosan solution into the egg yolk dilution and stir well, then let the solution stand for 3 hours. The precipitated mixed solution is divided into two layers, the bottom precipitate is the egg yolk lipid component, and the supernatant is the egg yolk water-soluble component, that is, the mixed solution of immunoglobulin IgY and other proteins.

Embodiment 3

[0028] In an environment of 4~50°C, after taking the yolk out of the egg, break the yolk membrane, measure 100m1 of the yolk stock solution, add 0.8g chitosan, 300m1 of 1% (V / V) acetic acid solution, stir well, and then put The mixture was allowed to settle overnight. The precipitated mixed solution is divided into two layers, the bottom precipitate is the egg yolk lipid component, and the supernatant is the egg yolk water-soluble component, that is, the mixed solution of immunoglobulin IgY and other proteins.

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Abstract

The invention discloses an application of chitosan to the yolk fatty deposition and derosination technology. The application method comprises the following steps: mechanically stirring the mixed solution of chitosan or chitosan solution and yolk stock solution or yolk diluted solution to separate the fatty components in the yolk from the water-soluble components, and standing for deposition and / or carrying out centrifugal separation to agglutinate and precipitate the fatty components in the yolk. A new application of the known compound chitosan is excavated in the invention, and a new application field is exploited. The effect of the method is reliable, and the method is simple and practical, can be used for removing all the fat efficiently and safely, causes no toxin and less influence to the protein component of the yolk and low IgY loss, has a low requirement for environmental conditions, does not need the large-scale equipment and instruments during application, uses less reagent, has low cost, is suitable for preparing the yolk antibody and the purified immunoglobulin in the library and is also suitable for the large-scale bath production of enterprises.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to the application of chitosan in egg yolk fat degreasing technology. Background technique [0002] Avian yolk antibody is a protein produced by poultry B lymphocytes stimulated by antigenic substances and deposited in yolk. The main component is immunoglobulin IgY (Immunoglobulin of yolk), which is transferred from poultry serum immunoglobulin. Equivalent to mammalian IgG (Immunoglobulin G), it has a typical immunoglobulin spatial conformation in structure, but does not contain a hinge region. Compared with mammalian immunoglobulin IgG, avian yolk antibodies have many advantages: First, due to the phylogenetic distance between birds and mammals, avian immunoglobulin IgY will not activate the complement system of mammals, and will not interact with mammals. Binding to Fc receptors on the surface of animal immunoglobulin IgG cells is more suitable for the prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K1/30
Inventor 梁永利周东顺吕素军金东庆王可洲
Owner 梁永利
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