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Method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor

A technology of xylose mother liquor and arabinose, which is applied in the field of simulated moving bed chromatography, can solve the problems of reducing product yield, resource and energy consumption, complex pretreatment, etc., achieves mature process conditions, realizes zero discharge, and has a wide range of applications Effect

Inactive Publication Date: 2013-02-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The mother liquor is a dark viscous liquid produced after two crystallizations of a high-concentration xylose and arabinose solution. Since the proportion of miscellaneous sugars such as galactose and glucose in the mother liquor increases after crystallization, the traditional crystallization process cannot continue Extract xylose and arabinose
Chinese patent 200510040433.0 discloses a method of removing glucose in xylose hydrolyzate or xylose mother liquor by Saccharomyces cerevisiae, and then using simulated moving bed technology to extract xylose or xylitol in the solution. This method cannot extract xylose with high added value. Arabinose and galactose can only be treated as miscellaneous sugar liquid at a price of 10%, which greatly reduces the production efficiency of enterprises
Chinese patent 200710014175.8 discloses a method for purifying xylose mother liquor by using a complex sequential simulated moving bed device to obtain a relatively high-purity xylose solution, but high value-added arabinose and galactose have not been effectively separated, significantly reducing the Utilization value of xylose mother liquor
Chinese patent 200910014319.9 discloses a method of introducing a remover into the xylose mother liquor to remove the glucose therein. Although this method obtains high value-added arabinose, the pretreatment is complicated and fails to effectively separate the xylose and semi-glucose in the mother liquor. lactose
Chinese patent CN102344467A discloses a method for producing xylose and arabinose by using the xylose mother liquor. This method not only separates the xylose and arabinose solution with low purity, but also fails to extract the galactose in the xylose mother liquor, which is concentrated and crystallized Miscellaneous sugars such as galactose greatly reduce the crystallization yield of xylose and arabinose during the process, resulting in a huge loss of resources and energy
[0003] Since the existing simulated moving bed method cannot effectively extract galactose in xylose fermentation broth or xylose mother liquor, the purity of the separated xylose and arabinose products is generally low, which greatly reduces the product yield in the subsequent crystallization process. rate, accompanied by a large amount of crystallization mother liquor

Method used

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  • Method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor
  • Method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0017] After the hydrolyzed liquid fermented, filter to remove impurities such as protein and yeast, ion exchange removes the salinity in the fermented liquid, obtain the xylose fermentation concentrated liquid (wherein xylose~60%; ~12%, arabinose ~21%), this solution enters the simulated moving bed separation, the separation conditions are as follows: the separation temperature is controlled at 70°C, the feed flow rate of the fermentation broth is 5cm / min, and the flow velocity of the eluting water is 20cm / min min, the system reaches equilibrium after 24 hours of feed operation, and the output of the three components is as follows:

[0018] 1) Xylose part: the refractive concentration is 27% (the volume is twice that of the feed solution); the purity of xylose in the solution is 91%, the purity of galactose is 3%, and the purity of arabinose is 1%;

[0019] 2) Galactose part: the refractive concentration is 7% (1.5 times the volume of the feed solution); the purity of xylose ...

Embodiment 2

[0023] After mixing the crystallization mother liquor of xylose, arabinose and galactose, the refractive concentration is 68% (wherein xylose ~ 62%; galactose ~ 10%, arabinose ~ 20%) into the simulated moving bed separation, the separation conditions are as follows: separation temperature The control is 75°C, the feeding flow rate of the crystallization mother liquor is 3cm / min, the flow rate of the eluting water is 12cm / min, and the system reaches equilibrium after 24 hours of feeding operation, and the discharge conditions of the three components are as follows:

[0024] 1) Xylose part: refractive concentration 32% (volume is 2 times of feed solution); xylose purity in solution is 93%, galactose purity is 2%, arabinose purity is 1%;

[0025] 2) Galactose part: the refractive concentration is 5% (1.5 times the volume of the feed solution); the purity of xylose in the solution is 24%, the purity of galactose is 65%, and the purity of arabinose is 5%;

[0026] 3) Arabinose part...

Embodiment 3

[0029] Fermentation concentrate and crystallization mother liquor are mixed according to the volume ratio of 3:1, and the refractive concentration of the mixed liquor is ~60% (including xylose ~60%, galactose ~11%, arabinose ~22%) into the simulated moving bed , the separation conditions are as follows: the separation temperature is controlled at 70 ° C, the feed flow rate of the crystallization mother liquor is 4 cm / min, the flow rate of the eluting water is 16 cm / min, the system reaches equilibrium after 24 hours of feed operation, and the discharge conditions of the three components as follows:

[0030] 1) Xylose part: the refractive concentration is 30% (twice the volume of the feed solution); the purity of xylose in the solution is 90%, the purity of galactose is 3%, and the purity of arabinose is 2%;

[0031] 2) Galactose part: the refractive concentration is 6% (1.5 times the volume of the feed solution); the purity of xylose in the solution is 22%, the purity of galact...

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Abstract

The invention provides a method for extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor. According to the method, a simulated moving bed chromatographic separation device of sugar alcohol separating resin special for filling is adopted, water serves as an eluent, the separation temperature is 30-90DEG C, the flow rate of feed liquid is controlled at 2-50cm / min, continuous separation and purification of the xylose, the arabinose and the galactose in the xylose fermentation broth or the xylose mother liquor is achieved, and high-purity xylose, arabinose and galactose products are obtained through concentration and crystallization. The method has the advantages of being wide in application range, good in separation effect, high in product purity, environment-friendly and the like.

Description

technical field [0001] The invention relates to a method for separating and extracting xylose, arabinose and galactose from xylose fermentation broth or xylose mother liquor, and belongs to the technical field of simulated moving bed chromatographic separation. Background technique [0002] Xylose fermentation liquid refers to the thin thick liquid obtained after the agricultural by-products such as corncobs, straw and bagasse are hydrolyzed with dilute acid and then fermented by microorganisms. The fermentation liquid contains a large amount of xylose and a small amount of arabinose and galactose. The mother liquor is a dark viscous liquid produced after two crystallizations of a high-concentration xylose and arabinose solution. Since the proportion of miscellaneous sugars such as galactose and glucose in the mother liquor increases after crystallization, the traditional crystallization process cannot continue Extract xylose and arabinose. Chinese patent 200510040433.0 dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/02C07H1/06
Inventor 张军伟刘志维王弦
Owner JIANGNAN UNIV
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