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Preparation method of redox probe for marking h-IGg (Human-Immunoglobulin G) impedance immunosensor

An immunosensor, label-free technology, applied in the direction of material resistance, material electrochemical variables, material inspection products, etc., can solve problems such as probes that cannot be used as electrochemical immunosensors, achieve low detection limit, good reproducibility, good stability

Inactive Publication Date: 2013-02-06
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common Redox Probe K 4 Fe(CN) 6 Often unavailable as probes for electrochemical immunosensors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] (1) Synthesis of β-mercaptoethylamine 3,5-dibromosalicylaldehyde Schiff base:

[0018] Under the condition of stirring, slowly dropwise add 10 mL of absolute ethanol solution containing 6 mmol β-mercaptoethylamine to 10 mL of absolute ethanol solution dissolved with 6 mmol 3,5-dibromosalicylaldehyde, and heat in a water bath at 60°C for 4 hours to obtain Light yellow precipitate, recrystallized with ethanol, and vacuum dried to obtain β-mercaptoethylamine 3,5-dibromosalicylaldehyde Schiff base powder.

[0019] (2) Synthesis of β-mercaptoethylamine 3,5-dibromosalicylaldehyde Schiff base nickel complex:

[0020] Make a mixed solvent of absolute ethanol and N,N'-dimethylformamide (DMF) at a volume ratio of 2:1, and dissolve 1 mmol of the β- Slowly add 8 mL of N,N'-dimethylformamide solution containing 2 mmol of nickel acetate dropwise into the above mixed solvent containing 3,5-dibromosalicylaldehyde Schiff base condensed with mercaptoethylamine, and heat in a water bath ...

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Abstract

The invention discloses a preparation method of a redox probe for marking an h-IGg (Human-Immunoglobulin G) impedance immunosensor. The preparation method comprises the following steps of: dropping an absolute ethyl alcohol solution containing beta-mercaptoethylamine into an absolute ethyl alcohol solution in which 3,5-dibromosalicylaldehyde is dissolved, performing water-bath heating, recrystallizing and drying in vacuum so as to obtain beta-mercaptoethylamine shrinkage 3,5-dibromosalicylaldehyde Schiff base; preparing the absolute ethyl alcohol and N,N-dimethylformamide into a mixture solvent in a volume ratio of 2:1, dropwise adding an N,N-dimethylformamide solution containing nickel acetate into the mixture solvent of the beta-mercaptoethylamine shrinkage 3,5-dibromosalicylaldehyde Schiff base, performing water-bath heating, recrystallizing and drying in vacuum so as to obtain a beta-mercaptoethylamine shrinkage 3,5-dibromosalicylaldehyde Schiff base complex; dissolving the beta-mercaptoethylamine shrinkage 3,5-dibromosalicylaldehyde Schiff base complex into the absolute ethyl alcohol, and further adding into nanogold sol. The redox probe is simple to prepare and has the advantages of low detection limit, good stability, good reproducibility and the like when being used for marking the h-IGg impedance immunosensor to detect the h-IGg content.

Description

technical field [0001] The invention relates to a preparation method of a redox probe used for an unlabeled h-IgG impedance type immunosensor. Background technique [0002] Immunoglobulin G (h-IgG) is the antibody with the highest content in human serum, accounting for 70-80% of serum immunoglobulins, and is the only immunoglobulin that can pass through the placenta. It has antibacterial, antiviral, and antitoxin properties. Plays an important role in the body's immune defense. At the same time, IgG is still an important substance to maintain human health. Many health foods rich in IgG, such as bovine colostrum tablets, have appeared on the market. In clinical testing, the change of IgG content is also an important indicator for judging lesions. Therefore, the establishment of a new type of rapid immunoglobulin G (h-IgG) detection method has a positive effect on food safety and bioanalysis detection. Label-free electrochemical immunosensors greatly simplify the preparatio...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/06G01N33/68
Inventor 刘峥袁帅赖丽燕
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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