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Method for preparing tobacco extracts through fermentation by utilizing immobilized enzyme preparations to be combined with acetic bacteria

A technology of tobacco extracts and immobilized enzymes, which is applied in the fields of tobacco, tobacco treatment, application, etc., can solve the problems such as the fermentation process that is not mentioned, and achieve the effects of strong acid production ability, improved aroma quality, and expanded application range

Active Publication Date: 2013-01-30
YUNNAN REASCEND TOBACCO TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many patents on acetic acid bacteria, but they are all concentrated in the fields of vinegar, wine and other brewing; patent 201110130665.0 proposes a preparation method of fermented Luo Han Guo extract for cigarettes, which mentions that the extract of Luo Han Guo fermented by acetic acid bacteria can be used as cigarettes Additive use; but these patents and articles do not mention the process of directly using acetic acid bacteria in tobacco extracts for fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Immobilized enzyme preparations are used to process tobacco extract raw materials: make enzyme liquid with 25% amylase, 0.2% enzyme stabilizer, 0.01% preservative and the rest of the water according to the mass ratio; cut the air glass fiber filter material into 3cm Put the circular paper piece into the pH7.5 phosphate buffer solution for 0.5h, take it out, put it into the enzyme solution and soak it for 6h at 20°C to make an immobilized enzyme preparation; add 200g of Zimbabwean tobacco leaf fragments to 5 times of water to sterilize Add 1g of immobilized enzyme preparation, mix well and stack at 35°C for 60 hours to obtain the processed product;

[0023] ②Acetic acid bacteria fermentation: Cultivate the acetic acid bacteria seed solution for use; add the obtained treated product to 20 times of water and then insert the acetic acid bacteria seed solution. The mass ratio of the acetic acid bacteria seed solution to the treated product is 10%, and culture at 25°C for 12...

Embodiment 2

[0026] Immobilized enzyme preparations to treat tobacco extract raw materials: use 30% protease, 5% enzyme stabilizer and the rest of the water to make enzyme liquid according to the mass ratio; cut the filter paper into 0.3cm circular paper pieces and put them in pH7.0 After soaking in phosphate buffer solution for 6 hours, take it out, put it into the enzyme solution and soak for 0.5 hours at 35°C to make immobilized enzyme preparation; add 200g of Turkish oriental tobacco powder to 2 times of water to sterilize, then insert 0.1g of immobilized enzyme preparation , after mixing, stacking at 45°C for 120 hours to obtain the processed product;

[0027] ②Acetic acid bacteria fermentation: Cultivate the acetic acid bacteria seed solution for use; add the obtained treated product to 10 times of water and then insert the acetic acid bacteria seed solution. The mass ratio of the acetic acid bacteria seed solution to the treated product is 0.5%, and culture at 38°C for 24 hours to ...

Embodiment 3

[0030] Immobilized enzyme preparations to treat tobacco extract raw materials: use 10% ligninase, 2% preservatives and the rest of water to make enzyme liquid according to the mass ratio; cut asbestos paper into 1.5cm circular paper pieces and put them in pH6. 5 Soak in phosphate buffer solution for 3 hours, take it out, put it in the enzyme solution and soak for 4 hours at 30°C to make an immobilized enzyme preparation; add 200g of crushed Brazilian tobacco stems to 3 times of water to sterilize, then insert 6.5g of the immobilized enzyme preparation, After mixing, it was stacked at 37°C for 24 hours to obtain the processed product.

[0031] ②Acetic acid bacteria fermentation: Cultivate the acetic acid bacteria seed solution for use; add the obtained treated product to 5 times of water and then insert the acetic acid bacteria seed solution. The mass ratio of the acetic acid bacteria seed solution to the treated product is 5%, and culture at 30°C for 60 hours to obtain fermen...

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Abstract

The invention provides a method for preparing tobacco extracts through fermentation by utilizing immobilized enzyme preparations to be combined with acetic bacteria. According to the method, the immobilized enzyme preparations are utilized for processing the tobacco extract raw materials to obtain processing products, then, the acetic bacteria are utilized for processing products through fermentation to obtain fermentation products, then, the fermentation products are subjected to concentration treatment, and the tobacco extracts are obtained. The method has the advantages that the contents of starch, protein, cellulose, lignin and pectin substance in the tobacco extracts can be fast and effectively reduced, in addition, the acid degree of the tobacco extracts can be properly improved, the fragrance and the smoking comfort of cigarette can be effectively improved, and the use range of the tobacco extracts can be improved.

Description

technical field [0001] The invention relates to the technical field of preparation methods of tobacco spices. Background technique [0002] In tobacco products, special attention is paid to the aroma and taste of tobacco, and some tobacco extracts are often added as a means to improve the quality of smoking and improve the style of the product. However, since the raw materials of tobacco extracts are usually some low-grade tobacco leaves or tobacco waste, the extracts have problems such as strong green miscellaneous gas, high irritation, and low tobacco aroma in the application of cigarettes, which restricts the extraction of tobacco. The use of substances in cigarette products. [0003] Starch, protein, cellulose, lignin and pectin are some macromolecular substances that can cause adverse suction effects. At present, the domestic and foreign methods for reducing these substances in cigarette raw materials are mainly through the application of microorganisms and enzyme pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
Inventor 毕薇李仙刘煜宇刘丽芬
Owner YUNNAN REASCEND TOBACCO TECH GRP
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