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Lysinibacillusfusiformis and method for degrading microcystis aeruginosa by using lysinibacillusfusiformis

A technology of Bacillus lysinus and Microcystis aeruginosa, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of increasing the amount of bacteria, increasing the cost, etc. Strong, low cost, high degradation rate effect

Active Publication Date: 2013-01-23
ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Journal of Fudan University (Natural Science Edition)" 2010, Volume 49, Issue 1, Page 94-98 reported that Wang Xiangrong isolated an alginolytic bacterium from yellow algae liquid, which was identified as Bacillus brevis), the bacteria showed a high removal rate of Microcystis aeruginosa only on the first day, and the removal rate was 71.6% under the condition of bacteria-algae ratio of 1:1, and the removal rate was almost 0 after 2 days, reaching 90 A removal rate of more than % can only increase the amount of bacteria, which increases the cost in actual engineering applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In this example, 100 mL of fresh Microcystis aeruginosa liquid was treated, and the concentration of chlorophyll a was 568.92 mg / m 3 , pH value is 7.0. The steps of the specific example are as follows: First, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (28 h), and then use a bacteria-to-algae ratio of 1:2, Inoculate 50 mL of the logarithmic phase bacterial liquid into the algae liquid, use sterile water as a blank control, and take a sample to determine the initial chlorophyll a concentration of 379.28 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 15.4 mg / m at 96 h 3 , the removal rate was as high as 95.94%, and the algae-dissolving rate remained at a relativel...

Embodiment 2

[0042] This example differs from Example 1 in that the treated Microcystis aeruginosa liquid has a different chlorophyll-a concentration and a different bacteria-to-algae ratio. In this example, 100 mL of fresh Microcystis aeruginosa liquid was treated, and the concentration of chlorophyll a was 505.82 mg / m 3 , pH value is 7.0. The steps of the specific example are as follows: First, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130r / min to the logarithmic phase (28 h), and then, according to the bacteria-to-algae ratio of 1:5, the 20 mL of the logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 421.52 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae li...

Embodiment 3

[0044] This example differs from Example 1 and Example 2 in that the concentration of chlorophyll a in the treated Microcystis aeruginosa liquid is different, and the ratio of bacteria and algae to bacteria is different. In this example, 100 mL of fresh Microcystis aeruginosa liquid was treated, and the concentration of chlorophyll a was 478.85 mg / m 3 , pH value is 7.0. The steps of the specific example are as follows: First, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (28 h), and then use a bacteria-to-algae ratio of 1:10, Inoculate 10 mL of the logarithmic phase bacterial liquid into the algae liquid, use sterile water as a blank control, and take a sample to determine the initial chlorophyll a concentration of 435.32 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorop...

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Abstract

The invention discloses lysinibacillusfusiformis and a method for degrading microcystis aeruginosa by using lysinibacillusfusiformis, belongs to the technical field of environment friendliness and water treatment, provides algicidal bacteria TL which is suggested to be classified and named ysinibacillusfusiformis with the collection number CGMCC NO.6108, and also provides a method for degrading microcystis aeruginosa by using lysinibacillusfusiformis. The characteristics of the separated algae-lysing bacteria lysinibacillusfusiformis are exerted by a mode of indirectly dissolving algae by secreting extracellular non-protease algae-killing substances and a mode of directly dissolving algae by contacting algae cells, and the lysinibacillusfusiformis has high degradation rate of microcystis aeruginosa, and has wide application prospect in the aspect of preventing cyanobacterial bloom in which microcystis aeruginosa is used as preponderant algae by a microbiological method.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water treatment, and in particular relates to a strain of lysine bacillus screened from a yellowish Microcystis aeruginosa liquid and a method for efficiently removing Microcystis aeruginosa by using the same. Background technique [0002] With the advancement of modern science and technology, the rapid development of industrial and agricultural automation, and the continuous improvement of residents' living standards, a large amount of nitrogen and phosphorus-rich production and domestic wastewater are continuously discharged into nearby rivers or lakes, which increases the nutrient load of water bodies and leads to eutrophication. The degree of globalization is increasing day by day, and cyanobacteria blooms occur frequently. The surface of the water body is covered by algae and cannot exchange air with the outside world, resulting in a decrease in reoxygenation rate, scarce dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 张文艺李秋艳陈雪珍李仁霞戴如娟郑泽鑫
Owner ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
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