Nano-fluorescent probe with quantum dots and application of nano-fluorescent probe with quantum dots on tumor targeting detection

Abstract

The invention belongs to the technical field of biomedicine detection, in particular to a method for preparing a nano-fluorescent probe with the quantum dots and an application of the nano-fluorescent probe with the quantum dots on tumor detection. According to the method, aiming at the defects that the traditional preparation method of the quantum dots is complicate to operate and high in cost, a simple and cheap water phase synthetic technique with the quantum dots is provided. The specific synthetic process comprises the synthesis of the water soluble quantum dots, the synthesis of a biocompatible modifier (mPEG) and a tumor cell targeting modifier (cRGD) with the sulfydryl functional groups and a direction reaction between the quantum dots and the two modifiers with the sulfydryl functional groups. According to the method provided by the invention, the preparing raw materials are easy to obtain, the whole preparation process is covalent modification under the conventional condition and the properties of the quantum dots and the modifiers are not changed, and the functions of in vivo long circulation and special cell targeting are simultaneously given for the quantum dots through a one-step reaction, so that the cost is effectively reduced. The modified fluorescent and bright quantum dots obtained by the method are about 5nm in particle size, strong in anti-photo-bleaching property, and have good biocompatibility. The transfection efficiency of the quantum dots on human breast cancer MDA-MB-231 cells can be effectively regulated and controlled by further adjusting the mole ratio of mPEG to cRGD on the surfaces of the quantum dots, so that the nano-fluorescent probe with quantum dots can be applied to the cell fluorescent imaging detection of the tumor targeting.

Description

1. Technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a preparation method of a tumor-targeted quantum dot fluorescent probe and its application in tumor detection. 2. Background technology [0002] Quantum dots (QDs) are a new type of nano-fluorescent material with good fluorescence emission properties and a size similar to organic dye molecules. By adjusting the size of quantum dots, it is possible to emit different colors of quantum dots at the same excitation wavelength, and has The better anti-photobleaching properties have great application prospects in the field of biomedical detection. At present, quantum dots are still in the research stage, and their low biocompatibility and tissue (cell) targeting greatly limit the clinical application of quantum dots. Therefore, efforts are being made to find effective ways to improve the toxicity of quantum dots and increase their abundance in specific tissu...

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved in the present invention is: aiming at the disadvantages of complex operation and high cost of the traditional preparation method of quantum dots, it is hoped to provide a simple quantum dot aqueous phase synthesis technology to realize the surface biocompatibility of quantum dots and target coordination. One-step modification of body molecules, and applied in tumor targeting detection

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