High-flux biomaterial-screening microfluidic chip

A technology of microfluidic chips and biological materials, applied in biomass post-treatment, biomass pre-treatment, biochemical cleaning devices, etc., can solve the problems of difficult-to-response cell changes, low efficiency, poor controllability, etc., and achieve improved detection Accuracy and detection efficiency, and the effect of reducing detection costs

Inactive Publication Date: 2013-01-16
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

This method has problems such as low efficiency and poor controllability, and indirect detection results are difficult to reflect real cell changes

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  • High-flux biomaterial-screening microfluidic chip
  • High-flux biomaterial-screening microfluidic chip

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with the accompanying drawings.

[0015] see Figure 1 to Figure 2 : A high-throughput biomaterial screening microfluidic chip is shown. Firstly, different biomaterials 11 and 12 to be screened are coated on the chip substrate 1 in a manner of precise spraying by a manipulator. The stem cells used for evaluating biological materials are added to the chip through the cell sampling element 3 of the microfluidic detection device 2; the cells enter the plasmid transfection element 5 through the channel 4, and the fluorescent biological probes for evaluating the induction effect of stem cells are transfected into the stem cells Inside, the cells are arranged in a single linear flow through the serpentine channel 6, and enter the cell capture element 9 to capture a single cell; the unidirectional drive microvalve 7 is used to add the cell growth nutrient solution through the biochemical reagent and growth fa...

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Abstract

The invention provides a high-flux biomaterial screening microfluidic chip which is characterized in that a microfluidic detection device is attached to a substrate, wherein the microfluidic detection device is provided with a cell sample introduction element from which more than 2 channels are led out; each channel is communicated with a plasmid transfection element, and the outlet of the plasmid transfection element is communicated with a serpentine tube; the outlet of each serpentine tube is divided into more than 2 channels; each channel is communicated with a cell capture element; and each cell capture element is communicated with a biochemical reagent and growth factor loading element. The invention can simultaneously detect cell integral shape and intracellular labeled protein activity variation under different biomaterial actions on one chip, and monitor the action effect of the biomaterial on the actual cells, thereby greatly lowering the detection cost, enhancing the detection accuracy and detection efficiency, and achieving the goal of biomaterial screening.

Description

technical field [0001] The invention relates to a biological chip, in particular to a high-throughput biological material screening microfluidic chip. technical background [0002] Biomaterials with tissue-inducing functions have become the focus of research in the field of contemporary biomaterials, the core of which is to induce the directional differentiation of stem cells in vivo through the optimization design of materials themselves. To screen the most suitable biological materials, the traditional method is mainly through the co-cultivation of materials and cells, using cell morphology changes, proliferation rate, and biochemical means to analyze a large number of digested cell lysates, or to observe the fixed cells by immunofluorescence technology. Some functional proteins are used to indirectly test and evaluate the influence of materials on cell behavior. This method has problems such as low efficiency and poor controllability, and the indirect detection results a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34
Inventor 廖晓玲刘波徐文峰
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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